CAJ | 학술논문

目的研究纤维母细胞活化蛋白(FAP)和转化生长因子β1(TGFβ1)在胃癌组织中的表达,并分析其与胃癌临床病理特征及淋巴结转移间的关系.方法采用免疫组化方法检测FAP和TGFβ1在42例胃癌和癌旁正常组织中的表达情况.结果 ①正常胃组织中FAP(-);胃癌组织中FAP主要表达于肿瘤相关纤维母细胞胞质中,且弥漫型胃癌表达显著高于肠型胃癌(P<0.05),并与临床病理分期、浸润深度和淋巴结转移呈正相关(P<0.05);②正常胃组织中TGFβ1为(-)或弱(+);胃癌组织中TGFβ1主要表达于瘤细胞胞质,在肿瘤间质纤维母细胞、血管内皮细胞和中性粒细胞偶有表达,且瘤细胞的表达强度与临床病理分期、浸润深度和淋巴结转移呈正相关(P<0.05);③ FAP在肿瘤相关纤维母细胞的表达与TGFβ1在肿瘤细胞的表达呈正相关(P<0.05).结论从FAP表达与TGFβ1表达的正相关性,推测其可能是通过肿瘤-间质相互作用促进肿瘤演进.FAP特异性表达于胃癌间质纤维母细胞,与多项临床病理指标,尤其是淋巴结转移和肿瘤的病理分型有关,可以作为预测胃癌淋巴结转移和预后的免疫病理学指标,有望成为抗胃癌治疗的一个新的靶向分子.
목적 연구섬유모세포활화단백(FAP)화전화생장인자β1(TGFβ1)재위암조직중적표체,병분석기여위암림상병리특정급림파결전이간적관계.방법 채용면역조화방법검측FAP화TGFβ1재42례위암화암방정상조직중적표체정황.결과 ①정상위조직중FAP(-);위암조직중FAP주요표체우종류상관섬유모세포포질중,차미만형위암표체현저고우장형위암(P<0.05),병여림상병리분기、침윤심도화림파결전이정정상관(P<0.05);②정상위조직중TGFβ1위(-)혹약(+);위암조직중TGFβ1주요표체우류세포포질,재종류간질섬유모세포、혈관내피세포화중성립세포우유표체,차류세포적표체강도여림상병리분기、침윤심도화림파결전이정정상관(P<0.05);③ FAP재종류상관섬유모세포적표체여TGFβ1재종류세포적표체정정상관(P<0.05).결론 종FAP표체여TGFβ1표체적정상관성,추측기가능시통과종류-간질상호작용촉진종류연진.FAP특이성표체우위암간질섬유모세포,여다항림상병리지표,우기시림파결전이화종류적병리분형유관,가이작위예측위암림파결전이화예후적면역병이학지표,유망성위항위암치료적일개신적파향분자.
Objective To study the expression of fibroblast activation protein ( FAP) and transforming growth factor β1 (TGFβ1) and their association with clinicopathologic features and lymph node metastasis in gastric carcinoma. Methods The expression of FAP and TGFβ1 was studied by immunohistochemistry. The correlation with various clinicopathologic parameters was analyzed. Results (1) FAP was mainly expressed in tumor associated fibroblasts and the level of its expression in diffuse-type of gastric carcinoma was higher than that in intestinal-type (P < 0.05); the expression was correlated with the TNM tumor stage, the depth of tumor invasion and lymph node metastases. (2) On the other hand, TGFβ1 mainly expressed in tumor cells and the level of its expression was correlated with TNM tumor stage, the depth of tumor invasion and lymph node metastases. (3) The expression of FAP in tumor associated fibroblasts was correlated with the expression of TGFβ1 in tumor cells. Conclusion FAP and TGFβ1 can serve as biomarkers for diagnosis and prediction of gastric carcinoma invasion and lymph node metastasis, as well as potential therapeutic targets in gastric carcinoma.