CAJ | 학술논문

为探讨利用重组肌肉抑制素促进肌肉生长的可能性,本研究原核表达并纯化了肌肉抑制素C-端重组蛋白.将雌性小鼠同雄性小鼠合笼,妊娠后分笼饲养.应用重组蛋白免疫雌性昆明白小鼠.利用间接ELISA法检测抗血清效价.每周测母鼠及仔鼠体质量1次.结果表明:(1)免疫后对照组和试验组母鼠体质最差异不显著(P>0.05).在妊娠后期(免疫后第11周),两组间体质量出现差异(P<0.05),试验组为(56.23±3.37)g,而对照组为(64.11±3.18)g.试验组体质量在第12周达到(79.16±3.72)g.而对照组为(75.23±3.44)g,差异极显著(P<0.01).分娩后,这种差异消失(P>0.05).(2)仔鼠出生体质量在2处理组间差异极显著(P<0.01),试验组仔鼠出生体质量比对照组增加25.8%左右;但在生长阶段仔鼠体质量差异有逐渐缩小的趋势.结论:应用肌肉抑制素C-端重组蛋白免疫母鼠可以显著提高仔鼠出生体质量.
위탐토이용중조기육억제소촉진기육생장적가능성,본연구원핵표체병순화료기육억제소C-단중조단백.장자성소서동웅성소서합롱,임신후분롱사양.응용중조단백면역자성곤명백소서.이용간접ELISA법검측항혈청효개.매주측모서급자서체질량1차.결과표명:(1)면역후대조조화시험조모서체질최차이불현저(P>0.05).재임신후기(면역후제11주),량조간체질량출현차이(P<0.05),시험조위(56.23±3.37)g,이대조조위(64.11±3.18)g.시험조체질량재제12주체도(79.16±3.72)g.이대조조위(75.23±3.44)g,차이겁현저(P<0.01).분면후,저충차이소실(P>0.05).(2)자서출생체질량재2처리조간차이겁현저(P<0.01),시험조자서출생체질량비대조조증가25.8%좌우;단재생장계단자서체질량차이유축점축소적추세.결론:응용기육억제소C-단중조단백면역모서가이현저제고자서출생체질량.
The potential of using recombinant MSTN as vaccine to improve muscle mass was ex-plored in this study. Firstly, the recombinant myostatin C-domain was obtained through prokary-otic expression and purification. Then female Kunming White mice after immunization with re-combinant C-domain were mated with male mice. The indirect ELISA assay was used to detect the titer of antibody against MSTN in mother mice. The body weights of mice were evaluated each week. The results showed: (1) The body weight of mother mice in experiment group didn't change significantly compared with that in control group (P>0. 05). But during the late preg-nancy (the 11th week after immunization), the increase of body weight of experiment group ex-ceeded significantly that in control group (P<0.05). The body weight was (56.23±3.37)g in experiment group,and (54.11±3.18)g in control group. In the 12th week after immunization, the body weight of experiment group was (79.16±3.72)g, and that of control group was (75.23 ±3.44)g. The former was significantly higher than the latter (P<0.01). After the delivery, the difference of body weight in two groups disappeared again (P>0.05) ; (2) The body weight of neonatal mice in experiment group was 25.8% higher than that in control group (P<0.01), but this difference decreased with the development of neonatal mice. These results proved that immu-nization of mother mice with recombinant MSTN C-domain protein could significantly increase the body weight of neonatal mice.