CAJ | 학술논문

从人的头发样品中筛选出1株产黑色素芽孢杆菌ZH168.对该菌株形态、生理生化特征、16S rDNA基因序列及全细胞蛋白电泳研究结果表明,ZH168菌株为枯草芽孢杆菌Bacillus subtilis.该菌产生的胞外黑色素在可见-紫外光区均有吸收,紫外区光吸收强,随着波长的减小,吸收增强,在210 nm左右有1个吸收高峰;红外光谱与合成的多巴黑色素很相近,具有黑色素吲哚结构的吸收峰.电子自旋共振谱是轻微不对称的典型单线一次微商波谱.综合以上结果确定该菌所产生的色素为黑色素.
종인적두발양품중사선출1주산흑색소아포간균ZH168.대해균주형태、생리생화특정、16S rDNA기인서렬급전세포단백전영연구결과표명,ZH168균주위고초아포간균Bacillus subtilis.해균산생적포외흑색소재가견-자외광구균유흡수,자외구광흡수강,수착파장적감소,흡수증강,재210 nm좌우유1개흡수고봉;홍외광보여합성적다파흑색소흔상근,구유흑색소신타결구적흡수봉.전자자선공진보시경미불대칭적전형단선일차미상파보.종합이상결과학정해균소산생적색소위흑색소.
A Bacillus strain ZH168 isolated from human hair can produce extracellular dark pigment.Base on the colony morphology,physiological and biochemical properties,16S rDNA gene sequence analysis and whole-cell protein electrophoresis,strain ZH168 was identified as Bacillus subtilis.The pigment produced by strain ZH168 has a district absorption in UV light,and as the wavelength decreased,the absorption increased.It has an absorption peak at approximately 210 nm.The pigment exhibited a similar infra-red absorbtion spectrum to synthetic melanin and contained indole structure.The electromagnetic spin resonance spectrum of the pigment was a slightly asymmetric singlet without hyperfine structure.These characteristics suggest that the pigment is menlanin.