CAJ | 학술논문

采用聚合酶链式反应(PCR)和直接测序的方法,测定了雉鸡甘肃亚种9个种群118个样本的线粒体DNA(mtDNA)控制区(D-loop) 1150个碱基长度的基因序列,43个变异位点产生51个单倍型.环境因子与种群遗传变异的相关性研究表明,序列变异与经度(r =0.725,P ＜0.05)、年平均温度(r=0.833,P＜0.05)呈显著正相关,核苷酸多样性与经度(r =0.686,P＜0.05)、年平均温度(r=0.844,P＜0.05)呈显著正相关.片段化与低温是导致雉鸡甘肃亚种遗传多样性的主要因素.序列变异及核苷酸多样性与温度的变异系数呈显著负相关(r序列=-0.679,P＜0.05; r核苷酸=-0.669,P＜0.05).温度越稳定地区雉鸡甘肃亚种的遗传多样性越高.
채용취합매련식반응(PCR)화직접측서적방법,측정료치계감숙아충9개충군118개양본적선립체DNA(mtDNA)공제구(D-loop) 1150개감기장도적기인서렬,43개변이위점산생51개단배형.배경인자여충군유전변이적상관성연구표명,서렬변이여경도(r =0.725,P ＜0.05)、년평균온도(r=0.833,P＜0.05)정현저정상관,핵감산다양성여경도(r =0.686,P＜0.05)、년평균온도(r=0.844,P＜0.05)정현저정상관.편단화여저온시도치치계감숙아충유전다양성적주요인소.서렬변이급핵감산다양성여온도적변이계수정현저부상관(r서렬=-0.679,P＜0.05; r핵감산=-0.669,P＜0.05).온도월은정지구치계감숙아충적유전다양성월고.
We used PCR and direct sequencing methods to investigate the relationship between environmental factors and population genetic variability of Strauch's pheasant ( Phasianus colchicus strauchi).A total of 1150 nucleotides of mitochondrial DNA control region were sequenced from 118 individuals in nine populations across the wide-distributions of the pheasant.Forty-three variable sites defined 51 haplotypes.Sequence variation increased with increasing longitude (r =0.725,P ＜ 0.05 ) and with annual average temperature ( r =0.833,P ＜ 0.05 ).Neucleotide diversity increased with longitude ( r =0.686,P ＜ 0.05 ) and with annual average temperature ( r =0.844,P ＜ 0.05 ).Fragmentation and low temperature were factors that contribute to the decline of genetic diversity.Sequence variation and neucleotide diversity increased with decreasing variation coefficient of temperature ( rsv =- 0.679,P ＜ 0.05 ; rπ =- 0.669,P ＜ 0.05 ).This shows that the more stable the temperature,the higher genetic diversity of Strauch's pheasant occurred.