CAJ | 학술논문

目的探讨粒细胞集落刺激因子(G-CSF)对大鼠脂肪基质细胞(ADASc)增殖的影响.方法将16只SD大鼠随机分为两组,即G-CSF组(8只)与对照组(8只).G-CSF组皮下注射G-CSF 20μg·kg-1·d-1,连用5 d;对照组皮下注射等量0.9%氯化钠注射液.每组分别于最后一次注射后6 h取大鼠腹膜后脂肪组织,分离、培养、鉴定ADASc,取两组第5代的ADASc,观测细胞生长的最大增殖倍数和倍增时间,流式细胞仪测定细胞周期分布.结果差异贴壁法培养出的第5代ADASc细胞为高纯度细胞群,表型特征为CD44+CD105-CD31-CD45-.G-CSF组与对照组大鼠ADASc最大增殖倍数、倍增时间、细胞周期Go-1细胞比例差异有统计学意义(P<0.05).G-CSF组大鼠ADASc与对照组比较,具有更大的增殖倍数和更短的倍增时间,处于Go-1周期的细胞数目也相对要少.结论差异贴壁法是培养ADASc一种比较好的方法;G-CSF可促进骨髓ADASc进入细胞增殖周期.
목적 탐토립세포집락자격인자(G-CSF)대대서지방기질세포(ADASc)증식적영향.방법 장16지SD대서수궤분위량조,즉G-CSF조(8지)여대조조(8지).G-CSF조피하주사G-CSF 20μg·kg-1·d-1,련용5 d;대조조피하주사등량0.9%록화납주사액.매조분별우최후일차주사후6 h취대서복막후지방조직,분리、배양、감정ADASc,취량조제5대적ADASc,관측세포생장적최대증식배수화배증시간,류식세포의측정세포주기분포.결과 차이첩벽법배양출적제5대ADASc세포위고순도세포군,표형특정위CD44+CD105-CD31-CD45-.G-CSF조여대조조대서ADASc최대증식배수、배증시간、세포주기Go-1세포비례차이유통계학의의(P<0.05).G-CSF조대서ADASc여대조조비교,구유경대적증식배수화경단적배증시간,처우Go-1주기적세포수목야상대요소.결론 차이첩벽법시배양ADASc일충비교호적방법;G-CSF가촉진골수ADASc진입세포증식주기.
Objective To investigate the effects of granulocyte colony-stimulating factor on proliferation of adipose-derived adult stromal cells(ADASc) in rats. Methods Sixteen SD rats were randomly divided into G-CSF group( n = 8) and control group( n = 8). The rats were subjected to subcutaneous injections of G-CSF at a dose of The ADASc were separated and cultured. Then: ( 1 ) The surface antigens of the ADASc were analyzed by flow cy- tometry. (2)The growth information of the ADASc were observed in vitro. (3)The generation cycle of the ADASc were investigated with the flow cytometry. Results ( 1 ) Flow cytometic detection of ADASe surface marks showed CD44+CD105-CD31-CD45-(2)The doubling generation time, the maximum proliferation multiple and the cell cycle distri- bution of ADASc in G-CSF group had significant difference from that of the control group. Conclusion The discrep- ancy adherence was a practical method to culture the ADASc;G-CSF treatment could promote ADASc re-entering into cell cycle.