中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
11期
976-978
,共3页
梅凤美%马正良%张睿%顾晓萍%张娟%周小芳%史长喜
梅鳳美%馬正良%張睿%顧曉萍%張娟%週小芳%史長喜
매봉미%마정량%장예%고효평%장연%주소방%사장희
大麻素%激动剂%JWH015%骨癌痛%机械痛敏
大痳素%激動劑%JWH015%骨癌痛%機械痛敏
대마소%격동제%JWH015%골암통%궤계통민
Cannabinoids%Agonist%JWH015%Bone cancer pain%Mechanical hyperalgesia
目的 观察鞘内注射大麻素受体2(CB2)激动剂JWH015对骨癌痛小鼠痛行为的影响.方法 雄性C3H/HeN小鼠32只随机分为4组(n=8):空白组(C),假手术组(s),DMSO(D,二甲基亚砜)组和JWH015(J)组.D组和J组在小鼠右侧股骨远端骨髓腔接种溶于最小必需培养基(α-MEM)的NCTC2472谘骨性纤维肉瘤细胞,建立骨癌痛模型;s组接种不含细胞的α-MEM;c组不予任何处理.在接种肿瘤细胞后的第14天按照分组D组和J组分别鞘内注射DMSO 5μl和溶于20%DMSO的JWH015 1 μg;S组注射DMSO.各组于接种前,接种后的3d、5d、7d、10d、14d以及给药后的1h、6h、24h、48h、72h测定小鼠的机械性缩足阈值(FWMT)和热缩腿潜伏期(FWTL).结果 与术后14d给药前的基础值[PWMT(0.45±0.09)g,PWTL(11.87±1.1)s]相比,鞘内注射JWH015后6h小鼠骨癌痛即缓解[PWMT(1.20±0.21)g,PWTL(15.35±2.42)s](P<0.05);24h缓解作用达到高峰[PWMT(1.85±0.28)g,PWTL(18.80±2.93)s](P<0.05);在72h作用衰退到给药前水平[PWMT(0.48±0.10)g,PWTL(11.83±1.19)s]P>0.05).结论 鞘内注射JWH015能够显著改善骨癌痛小鼠的痛行为.
目的 觀察鞘內註射大痳素受體2(CB2)激動劑JWH015對骨癌痛小鼠痛行為的影響.方法 雄性C3H/HeN小鼠32隻隨機分為4組(n=8):空白組(C),假手術組(s),DMSO(D,二甲基亞砜)組和JWH015(J)組.D組和J組在小鼠右側股骨遠耑骨髓腔接種溶于最小必需培養基(α-MEM)的NCTC2472諮骨性纖維肉瘤細胞,建立骨癌痛模型;s組接種不含細胞的α-MEM;c組不予任何處理.在接種腫瘤細胞後的第14天按照分組D組和J組分彆鞘內註射DMSO 5μl和溶于20%DMSO的JWH015 1 μg;S組註射DMSO.各組于接種前,接種後的3d、5d、7d、10d、14d以及給藥後的1h、6h、24h、48h、72h測定小鼠的機械性縮足閾值(FWMT)和熱縮腿潛伏期(FWTL).結果 與術後14d給藥前的基礎值[PWMT(0.45±0.09)g,PWTL(11.87±1.1)s]相比,鞘內註射JWH015後6h小鼠骨癌痛即緩解[PWMT(1.20±0.21)g,PWTL(15.35±2.42)s](P<0.05);24h緩解作用達到高峰[PWMT(1.85±0.28)g,PWTL(18.80±2.93)s](P<0.05);在72h作用衰退到給藥前水平[PWMT(0.48±0.10)g,PWTL(11.83±1.19)s]P>0.05).結論 鞘內註射JWH015能夠顯著改善骨癌痛小鼠的痛行為.
목적 관찰초내주사대마소수체2(CB2)격동제JWH015대골암통소서통행위적영향.방법 웅성C3H/HeN소서32지수궤분위4조(n=8):공백조(C),가수술조(s),DMSO(D,이갑기아풍)조화JWH015(J)조.D조화J조재소서우측고골원단골수강접충용우최소필수배양기(α-MEM)적NCTC2472자골성섬유육류세포,건립골암통모형;s조접충불함세포적α-MEM;c조불여임하처리.재접충종류세포후적제14천안조분조D조화J조분별초내주사DMSO 5μl화용우20%DMSO적JWH015 1 μg;S조주사DMSO.각조우접충전,접충후적3d、5d、7d、10d、14d이급급약후적1h、6h、24h、48h、72h측정소서적궤계성축족역치(FWMT)화열축퇴잠복기(FWTL).결과 여술후14d급약전적기출치[PWMT(0.45±0.09)g,PWTL(11.87±1.1)s]상비,초내주사JWH015후6h소서골암통즉완해[PWMT(1.20±0.21)g,PWTL(15.35±2.42)s](P<0.05);24h완해작용체도고봉[PWMT(1.85±0.28)g,PWTL(18.80±2.93)s](P<0.05);재72h작용쇠퇴도급약전수평[PWMT(0.48±0.10)g,PWTL(11.83±1.19)s]P>0.05).결론 초내주사JWH015능구현저개선골암통소서적통행위.
Objective To investigate effect of intrathecal injection JWH015 on pain behavior in a mouse model of bone cancer pain. Methods 32 male C3H/HeN mice weighing 20～25g were randomly divided into 4 groups (n = 8) : control group(C) ,sham group(S) ,DMSO group(D) and JWH015 group(J). Group D and group J were induced mouse models of bone cancer pain by intra-right-femur inoculations of osteolytic NCTC2472 cells while group S were injected only α-MEM and group C were done nothing. On the 14d after inoculations, group D and group J accepted intrathecal injection of DMSO and 1 μg JWH015 which dissolved in 5 μl DMSO, respectively. Mouse accepted pain behavior tests including paw withdrawal mechanical threshold (FWMT) and paw withdrawal thermal latency ( FWTL) before and at 3d ,5d,7d, 10d, 14d after inoculations. Also, the same tests were performed at 1h, 6h, 24h, 48h, 72h, after administration. Results Compared with before administration [PWMT(0. 45 ± 0. 09) g, PWTL( 11.87 ± 1.1) s ] , the mice of group J showed significant pain relief at 6h[ PWMT(1.20 ± 0.21) g, PWTL(15.35 ± 2. 42) s, P < 0. 05 ], the effect of JWH015 reached its peak at 24h [ PWMT(1. 85 ± 0. 28) g, PWTL(18.80 ±2.93)s, P<0.05], while declined at 72h [PWMT(0.48 ±0.10)g, PWTL(11.83 ±1.19)s, P> 0.05]. No obvious change of pain behavior was observed in group D (P > 0.05 ). Conclusion Intrathecal injection JWH015 attenuated bone cancer pain.
