中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
2期
127-130
,共4页
陈衍辉%张恒辉%王艳%廖维甲%覃理灵%谢兴旺%费然%王雪艳%梅铭惠%魏来%陈红松
陳衍輝%張恆輝%王豔%廖維甲%覃理靈%謝興旺%費然%王雪豔%梅銘惠%魏來%陳紅鬆
진연휘%장항휘%왕염%료유갑%담리령%사흥왕%비연%왕설염%매명혜%위래%진홍송
癌,肝细胞%多态性,单核苷酸%FOXP3基因
癌,肝細胞%多態性,單覈苷痠%FOXP3基因
암,간세포%다태성,단핵감산%FOXP3기인
Carcinoma,hepatocellular%Polymorphism,single nucleotide%FOXP3 gene
目的 探讨转录因子FOXP3基因单核苷酸多态性(single nucleotide polymorphisms,SNPs)与原发性肝细胞癌(hepatocellular carcinoma,HCC)的关系.方法 采用飞行时间质谱法( MALDI-TOF)对392例肝癌患者和372例健康对照者FOXP3基因rs2280883和rs3761549位点单核苷酸多态性进行分析. 结果 肝癌组FOXP3基因SNP位点rs3761549 C等位基因的频率明显高于健康对照组(OR=1.32,95％ CI 1.03 ～ 1.70,P=0.027).肝癌患者FOXP3基因在rs2280883位点上出现TT基因型和在rs3761549位点上出现CC基因型的比例较健康对照者高,分别为79.6％和77.6％,并且携带rs2280883 TT基因型或者rs3761549 CC基因型的患者发生肝癌的风险也较高(OR =1.53,95％CI1.10～2.14；OR=1.92,95％CI1.39 ～2.64；P＜0.001).分层分析肝癌患者的临床资料发现,rs3761549位点上CC基因型与原发性肝细胞癌发病的关联在发生门静脉癌栓的患者中更加显著(x2=5.578,P=0.018),而且rs3761549位点上Tr/CT基因型与原发性肝细胞癌发病的关联在出现肝癌复发的患者中更加显著(x2=6.561,P=0.010).结论 FOXP3基因rs2280883和rs3761549位点的多态性与原发性肝细胞癌发病具有相关性,其中rs3761549位点CC基因型和TT/CT基因型分别与原发性肝细胞癌患者的门静脉癌栓发生和肝癌复发具有相关性.
目的 探討轉錄因子FOXP3基因單覈苷痠多態性(single nucleotide polymorphisms,SNPs)與原髮性肝細胞癌(hepatocellular carcinoma,HCC)的關繫.方法 採用飛行時間質譜法( MALDI-TOF)對392例肝癌患者和372例健康對照者FOXP3基因rs2280883和rs3761549位點單覈苷痠多態性進行分析. 結果 肝癌組FOXP3基因SNP位點rs3761549 C等位基因的頻率明顯高于健康對照組(OR=1.32,95％ CI 1.03 ～ 1.70,P=0.027).肝癌患者FOXP3基因在rs2280883位點上齣現TT基因型和在rs3761549位點上齣現CC基因型的比例較健康對照者高,分彆為79.6％和77.6％,併且攜帶rs2280883 TT基因型或者rs3761549 CC基因型的患者髮生肝癌的風險也較高(OR =1.53,95％CI1.10～2.14；OR=1.92,95％CI1.39 ～2.64；P＜0.001).分層分析肝癌患者的臨床資料髮現,rs3761549位點上CC基因型與原髮性肝細胞癌髮病的關聯在髮生門靜脈癌栓的患者中更加顯著(x2=5.578,P=0.018),而且rs3761549位點上Tr/CT基因型與原髮性肝細胞癌髮病的關聯在齣現肝癌複髮的患者中更加顯著(x2=6.561,P=0.010).結論 FOXP3基因rs2280883和rs3761549位點的多態性與原髮性肝細胞癌髮病具有相關性,其中rs3761549位點CC基因型和TT/CT基因型分彆與原髮性肝細胞癌患者的門靜脈癌栓髮生和肝癌複髮具有相關性.
목적 탐토전록인자FOXP3기인단핵감산다태성(single nucleotide polymorphisms,SNPs)여원발성간세포암(hepatocellular carcinoma,HCC)적관계.방법 채용비행시간질보법( MALDI-TOF)대392례간암환자화372례건강대조자FOXP3기인rs2280883화rs3761549위점단핵감산다태성진행분석. 결과 간암조FOXP3기인SNP위점rs3761549 C등위기인적빈솔명현고우건강대조조(OR=1.32,95％ CI 1.03 ～ 1.70,P=0.027).간암환자FOXP3기인재rs2280883위점상출현TT기인형화재rs3761549위점상출현CC기인형적비례교건강대조자고,분별위79.6％화77.6％,병차휴대rs2280883 TT기인형혹자rs3761549 CC기인형적환자발생간암적풍험야교고(OR =1.53,95％CI1.10～2.14；OR=1.92,95％CI1.39 ～2.64；P＜0.001).분층분석간암환자적림상자료발현,rs3761549위점상CC기인형여원발성간세포암발병적관련재발생문정맥암전적환자중경가현저(x2=5.578,P=0.018),이차rs3761549위점상Tr/CT기인형여원발성간세포암발병적관련재출현간암복발적환자중경가현저(x2=6.561,P=0.010).결론 FOXP3기인rs2280883화rs3761549위점적다태성여원발성간세포암발병구유상관성,기중rs3761549위점CC기인형화TT/CT기인형분별여원발성간세포암환자적문정맥암전발생화간암복발구유상관성.
Objective To investigate the correlation between single nucleotide polymorphisms (SNPs) of FOXP3 gene and the susceptibility of hepatocellular carcinoma (HCC). Methods Two SNPs rs2280883 and rs3761549 of FOXP3 gene in 392 HCC patients and 372 healthy controls were analyzed by Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF).Results At rs3761549,C allele frequency was significantly higher ( OR =1.32,95％ CI 1.03 -1.70,P =0.027) in HCC patients than healthy controls.Compared with healthy controls,HCC patients had higher frequencies of TT genotype (79.6％ ) at rs2280883 or CC genotype (77.6％) at rs3761549 of FOXP3 gene.Patients carrying rs2280883 TT genotype ( OR =1.53,95％ CI 1.10 - 2.14,P ＜ 0.00001 ) or rs3761549 CC genotype ( OR =1.92,95％ CI 1.39 - 2.64,P ＜ 0.00001 ) were more susceptible to HCC.Stratified analysis showed that rs3761549 CC genotype was significantly associated with higher incidence of portal vein tumor thrombus ( x2 =5.578,P =0.018 ),and rs3761549 TT/CT genotype was significantly associated with higher rate of tumor recurrence in HCC patients (x2 =6.561,P =0.010).Conclusions FOXP3 gene polymorphisms at rs2280883 and rs3761549 might be associated with increased susceptibility to HCC. rs3761549,CC genotype and TT/CT genotype were respectively associated with higher incidence of portal vein tumor thrombus and tumor recurrence in HCC patients.
