中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2012年
1期
31-33
,共3页
宫连凤%刘娟%韩文清%崔伟红%孙振璐%姜梅
宮連鳳%劉娟%韓文清%崔偉紅%孫振璐%薑梅
궁련봉%류연%한문청%최위홍%손진로%강매
肝炎病毒,戊型%基因型%序列分析%模型,动物
肝炎病毒,戊型%基因型%序列分析%模型,動物
간염병독,무형%기인형%서렬분석%모형,동물
Hepatitis E virus%Genotype%Sequence analysis%Models,animal
目的 调查烟台市沿海地区人源与猪源戊型肝炎病毒(HEV)基因型别的相关性.方法 应用逆转录一巢式聚合酶联反应( RT-nPCR)方法对当地急性散发戊型肝炎患者、正常人群中抗HEV-IgM阳性者和当地养猪场的猪进行HEV RNA检测,并对HEV RNA阳性标本进行克隆测序和序列分析.结果 16例急性散发戊型肝炎患者中有7例粪便标本HEV RNA阳性;51份lgM阳性正常人群血清标本中有1份HEV RNA阳性;34份猪胆汁标本中有1份HEV RNA阳性.序列分析发现该地区HEV人株与猪株在ORF2部分区域的核苷酸序列同源性为87%~ 98.1%.7株患者的戊肝病毒基因型和1株猪的戊肝病毒基因型均为Ⅳ型,基因序列同源性在87% ~ 98.1%之间;其中有6例患者和猪的基因序列同源性在93.9% ~98.1%之间,为Ⅳ型a亚型;1例患者和猪的基因序列同源性为87%,为Ⅳ型d亚型.正常人群的1例戊肝病毒基因型为Ⅰ型d亚型.该地区人与猪HEV的ORF2的部分基因片段与HEV Ⅰ~Ⅳ型的代表株进行比较,核苷酸序列同源性分别是82.5%~100%,81.7% ~92.9%,81.4% ~93.9%,84.9% ~ 100%.结论 该地区人群中流行的HEV存在2个基因型3个亚型,主要以基因Ⅳa型为主,与猪群中流行的HEV基因Ⅳa型同源性较高;HEV Ⅰ型在人群中散在存在.
目的 調查煙檯市沿海地區人源與豬源戊型肝炎病毒(HEV)基因型彆的相關性.方法 應用逆轉錄一巢式聚閤酶聯反應( RT-nPCR)方法對噹地急性散髮戊型肝炎患者、正常人群中抗HEV-IgM暘性者和噹地養豬場的豬進行HEV RNA檢測,併對HEV RNA暘性標本進行剋隆測序和序列分析.結果 16例急性散髮戊型肝炎患者中有7例糞便標本HEV RNA暘性;51份lgM暘性正常人群血清標本中有1份HEV RNA暘性;34份豬膽汁標本中有1份HEV RNA暘性.序列分析髮現該地區HEV人株與豬株在ORF2部分區域的覈苷痠序列同源性為87%~ 98.1%.7株患者的戊肝病毒基因型和1株豬的戊肝病毒基因型均為Ⅳ型,基因序列同源性在87% ~ 98.1%之間;其中有6例患者和豬的基因序列同源性在93.9% ~98.1%之間,為Ⅳ型a亞型;1例患者和豬的基因序列同源性為87%,為Ⅳ型d亞型.正常人群的1例戊肝病毒基因型為Ⅰ型d亞型.該地區人與豬HEV的ORF2的部分基因片段與HEV Ⅰ~Ⅳ型的代錶株進行比較,覈苷痠序列同源性分彆是82.5%~100%,81.7% ~92.9%,81.4% ~93.9%,84.9% ~ 100%.結論 該地區人群中流行的HEV存在2箇基因型3箇亞型,主要以基因Ⅳa型為主,與豬群中流行的HEV基因Ⅳa型同源性較高;HEV Ⅰ型在人群中散在存在.
목적 조사연태시연해지구인원여저원무형간염병독(HEV)기인형별적상관성.방법 응용역전록일소식취합매련반응( RT-nPCR)방법대당지급성산발무형간염환자、정상인군중항HEV-IgM양성자화당지양저장적저진행HEV RNA검측,병대HEV RNA양성표본진행극륭측서화서렬분석.결과 16례급성산발무형간염환자중유7례분편표본HEV RNA양성;51빈lgM양성정상인군혈청표본중유1빈HEV RNA양성;34빈저담즙표본중유1빈HEV RNA양성.서렬분석발현해지구HEV인주여저주재ORF2부분구역적핵감산서렬동원성위87%~ 98.1%.7주환자적무간병독기인형화1주저적무간병독기인형균위Ⅳ형,기인서렬동원성재87% ~ 98.1%지간;기중유6례환자화저적기인서렬동원성재93.9% ~98.1%지간,위Ⅳ형a아형;1례환자화저적기인서렬동원성위87%,위Ⅳ형d아형.정상인군적1례무간병독기인형위Ⅰ형d아형.해지구인여저HEV적ORF2적부분기인편단여HEV Ⅰ~Ⅳ형적대표주진행비교,핵감산서렬동원성분별시82.5%~100%,81.7% ~92.9%,81.4% ~93.9%,84.9% ~ 100%.결론 해지구인군중류행적HEV존재2개기인형3개아형,주요이기인Ⅳa형위주,여저군중류행적HEV기인Ⅳa형동원성교고;HEV Ⅰ형재인군중산재존재.
Objective Survey of the coastal city of Yantai,from human and swine hepatitis E virus (HEV) genotype correlation. Method Application of reverse transcription nested polymerase chain reaction (RT - nPCR) method for local acute sporadic hepatitis E patients,normal population of HEV-IgM positive and local pig farm pigs were HEV RNA detection.And HEV RNA positive samples for cloning sequencing and sequence analysis.Results In 16 patients with acute sporadic hepatitis E in 7 cases of RNA positive stool specimens of HEV; 51 lgM positive sera of normal people in specimens with 1 HEV RNA positive; 34 pig bile specimens with 1 HEV RNA positive. Sequence analysis revealed the region HEV strains and swine strains in the ORF2 region of nucleotide sequence homology is 87% -98.1%.7 strains of hepatitis E virus genotype in patients and 1 strains of swine hepatitis E virus genotypes are type Ⅳ,gene sequence homology between the 87% -98.1% ; there were 6 patients and porcine gene sequence homology in 93.9% -98.1% between,for type a subtype; 1 patients and porcine gene sequence homology in 87%,for the type D subtype.Normal population of 1 cases of hepatitis E virus genotype for I type D subtype.Human and porcine HEV ORF2 gene fragment and HEV part Ⅰ-Ⅳ representative strains were compared,and the nucleotide sequence homology were 82.5% - 100%,81.7% - 92.9%,81.4% - 93.9%,84.9% - 100%.Conclusion The area population prevalence of HEV in the presence of 2 genotype 3 subtype genes,mainly to Ⅳ A,in pigs with popular HEV gene with a high homology; HEV type Ⅰ in the crowd disperses in the presence of.