复旦学报(医学版)
複旦學報(醫學版)
복단학보(의학판)
JOURNAL OF FUDAN UNIVERSITY
2001年
1期
21-23,31
,共4页
陈红%佘振珏%童夙明%褚云鸿
陳紅%佘振玨%童夙明%褚雲鴻
진홍%사진각%동숙명%저운홍
神经生长因子%颈上神经节%腓神经%再生
神經生長因子%頸上神經節%腓神經%再生
신경생장인자%경상신경절%비신경%재생
目的 应用形态学方法观察研究神经生长因子(NGF)对长春新碱(VCR)损伤的小鼠颈上神经节和钳夹损伤的大鼠腓神经修复再生作用。方法 颈上神经节：出生2d的昆明种小鼠随机分为实验组、对照组和空白对照组。实验组皮下注射VCR(0.2 mol/L～10 μl/g体重)和NGF(2、5、10μg/g体重)，对照组仅注射VCR，每天1次，连续4 d。光镜下测量颈上神经节横径并且观察神经节细胞形态变化。周围神经：将钳夹损伤腓神经的SD大鼠随机分为实验组和对照组，并从损伤后第1天在近损伤处分别肌注NGF(2、4、8μg/kg体重)和生理盐水，每天1次，连续12 d。取腓神经和趾长伸肌，光镜观察，并计数损伤处远端和近端神经纤维数量。结果VCR可损伤颈上神经节，神经节横径缩小，节细胞凋亡解体；NGF则可改善VCR的损害作用，神经节横径增大61％～95％，节细胞数明显增多59％～70％，细胞凋亡现象显著减轻，改善程度与NGF剂量相关。NGF对腓神经再生和趾长伸肌形态变化也有明显改善作用，尤以大剂量NGF的作用更显著。结论 NGF对长春新碱损伤的小鼠颈上神经节和经钳夹损伤的大鼠腓神经有明显的促修复作用。
目的 應用形態學方法觀察研究神經生長因子(NGF)對長春新堿(VCR)損傷的小鼠頸上神經節和鉗夾損傷的大鼠腓神經脩複再生作用。方法 頸上神經節：齣生2d的昆明種小鼠隨機分為實驗組、對照組和空白對照組。實驗組皮下註射VCR(0.2 mol/L～10 μl/g體重)和NGF(2、5、10μg/g體重)，對照組僅註射VCR，每天1次，連續4 d。光鏡下測量頸上神經節橫徑併且觀察神經節細胞形態變化。週圍神經：將鉗夾損傷腓神經的SD大鼠隨機分為實驗組和對照組，併從損傷後第1天在近損傷處分彆肌註NGF(2、4、8μg/kg體重)和生理鹽水，每天1次，連續12 d。取腓神經和趾長伸肌，光鏡觀察，併計數損傷處遠耑和近耑神經纖維數量。結果VCR可損傷頸上神經節，神經節橫徑縮小，節細胞凋亡解體；NGF則可改善VCR的損害作用，神經節橫徑增大61％～95％，節細胞數明顯增多59％～70％，細胞凋亡現象顯著減輕，改善程度與NGF劑量相關。NGF對腓神經再生和趾長伸肌形態變化也有明顯改善作用，尤以大劑量NGF的作用更顯著。結論 NGF對長春新堿損傷的小鼠頸上神經節和經鉗夾損傷的大鼠腓神經有明顯的促脩複作用。
목적 응용형태학방법관찰연구신경생장인자(NGF)대장춘신감(VCR)손상적소서경상신경절화겸협손상적대서비신경수복재생작용。방법 경상신경절：출생2d적곤명충소서수궤분위실험조、대조조화공백대조조。실험조피하주사VCR(0.2 mol/L～10 μl/g체중)화NGF(2、5、10μg/g체중)，대조조부주사VCR，매천1차，련속4 d。광경하측량경상신경절횡경병차관찰신경절세포형태변화。주위신경：장겸협손상비신경적SD대서수궤분위실험조화대조조，병종손상후제1천재근손상처분별기주NGF(2、4、8μg/kg체중)화생리염수，매천1차，련속12 d。취비신경화지장신기，광경관찰，병계수손상처원단화근단신경섬유수량。결과VCR가손상경상신경절，신경절횡경축소，절세포조망해체；NGF칙가개선VCR적손해작용，신경절횡경증대61％～95％，절세포수명현증다59％～70％，세포조망현상현저감경，개선정도여NGF제량상관。NGF대비신경재생화지장신기형태변화야유명현개선작용，우이대제량NGF적작용경현저。결론 NGF대장춘신감손상적소서경상신경절화경겸협손상적대서비신경유명현적촉수복작용。
Purpose The regeneration effects of nerve growth factor(NGF)on superior cervical ganglia innewborn mice destroyed by vincristine(VCR)and peroneal nerve of adult rats destroyed by grip were studiedby morphological methods. Methods Superior cervical ganglia. The Qunming newborn mice at 2 dayswere divided into 3 groups: experiment, control and blank. The experiment animals were injected with VCR,10 μl/g of body weight at a concentration of 0.02 mmol/L. Simultaneously, the NGF was injected 2,5,10μg/g of body weight, respectively. But the control animals were only injected with VCR at the same dose.The blank control animals weren' t treated anything. All of these chemicals were injected once a day for 4days. 24h after the last injection, the superior cervical ganglia were dissected out and analyzed their size andmorphology. Peripheral nerve. The peroneal nerve of SD adult rats were destroyed by grip, and divided into2 groups: experiment and control. The experiment rats were injected with NGF 2,4 and 8 μg/kg of bodyweight respectively, near the gripped nerve,once a day for 12 days after 24 h of the injury. 24 h after the lastinjection, the perone al nerve and extensor longus digitorum were dissected out and analyzed their morphologyand counted the number of nerve fiber at proximal and distal injury. Results VCR injection in newbommice produced severe atrophy of superior cervical ganglia. And the neuronal cells apoptosed and decomposed.Simultaneous injections of NGF prevented the noxious effects of VCR, and resulted in an increase intransverse diameter from 61 to 95 percent and the total number of neuronal cells from 59 to 70 percent. Thisimproved degree was related to the dose of NGF. Furthermore, NGF obviously improved the structure of peroneal nerve and extensor longus digitorum. And this effect was the best in the high dosage. ConclusionsNerve growth factor has an obvious regeneration effects in superior cervical ganglia of newborn mice destroyed by VCR and peroneal nerve of rat destroyed by grip.
