CAJ | 학술논문

目的检验5种中草药提取物(5ECH)对大鼠的减肥降脂作用及其机制.方法喂饲高脂饲料制备肥胖高脂大鼠模型,5ECH各设两个剂量为实验组,曲美和血脂康为阳性对照组,羧甲基纤维素钠为阴性对照组,各组均ig相应药物.TACHI-7170A自动生化分析仪测定血浆甘油三酯(TG)和总胆固醇(TC)含量;分光光度法和同位素法测定脂肪酸合成酶(FAS)及脂酰辅酶A合酶(ACS)活性.常规称重法测定各组大鼠体重增长指数(BWI)、脂肪质量(BF)及摄食量(FI).结果5ECH对大鼠BWI、BF、TG、TC和FI具有剂量依赖性抑制作用;并剂量依赖性地抑制FAS活性、提高ACS活性.BWI与FI具有高度相关性,TG和TC水平与FAS及ACS具有高度相关性,BF与FI及两种酶的活性具有高度相关性.结论5ECH通过抑制食欲、抑制FAS活性和促进ACS活性而对高脂饮食诱导的肥胖大鼠具有显著的剂量依赖性减肥降脂作用.
목적검험5충중초약제취물(5ECH)대대서적감비강지작용급기궤제.방법위사고지사료제비비반고지대서모형,5ECH각설량개제량위실험조,곡미화혈지강위양성대조조,최갑기섬유소납위음성대조조,각조균ig상응약물.TACHI-7170A자동생화분석의측정혈장감유삼지(TG)화총담고순(TC)함량;분광광도법화동위소법측정지방산합성매(FAS)급지선보매A합매(ACS)활성.상규칭중법측정각조대서체중증장지수(BWI)、지방질량(BF)급섭식량(FI).결과5ECH대대서BWI、BF、TG、TC화FI구유제량의뢰성억제작용;병제량의뢰성지억제FAS활성、제고ACS활성.BWI여FI구유고도상관성,TG화TC수평여FAS급ACS구유고도상관성,BF여FI급량충매적활성구유고도상관성.결론5ECH통과억제식욕、억제FAS활성화촉진ACS활성이대고지음식유도적비반대서구유현저적제량의뢰성감비강지작용.
Object To evaluate the effects of the extracts from five kinds of Chinese herbs (5ECH) on weight-reduction and fat-decrease in rats, as well as their mechanisms. Methods Rats were fed by highcalorie food and administrated with two different doses of 5ECH as experimental groups, with Qumei and Xuezhika ng as positive control groups, and with carboxymethyl cellulose sodium as negative control group by ig administration with relevant drugs in each groups. Concentrations of triglycerides (TG) and total cholesterol (TC) in the blood were determined by a HITACHI-7170A auto-biochemical analyzer. The activities of fatty acid synthase (FAS) and acyl-coenzyme A synthetase (ACS) were determined by spectrophotometry and isotopic-labeling, respectively. The items of body weight increase (BWI), body fat (BF), and food intake (FI) were determined by regular weighing. ResuLts 5ECH reduces BWI, BF, TG,TC, and FI in a dose-dependent manner inhibits FAS activity, and enhances ACS activity in a dose-dependent manner. The BWI is highly correlated to the FI; the TG and TC levels in the blood are highly correlated to the activities of FAS and ACS, and the BF is highly correlated to both the FI and the two enzymes′activities. Conclusion The 5ECH in this research has significant weight-reduction and fatdecerase effects on rats. The effects are associated with appetite suppression, FAS inhibition, and ACS activation.