CAJ | 학술논문

目的:观察2型糖尿痛大鼠肾组织PPARα/δ/γ蛋白的表达及小檗碱对它们的影响.方法:小剂量注射链脲菌素(35 mg·kg-1,ip)加高糖高脂饲料饲养16周建立2型糖尿病大鼠模型,随后16周每天分别给予低中高剂量小檗碱75、150、300mg·kg-1、非诺贝特100mg·kg1 和罗格列酮4mg·kg-1,处死大鼠后用免疫组化技术检测肾脏组织中PPARα/δ/γ的表达.结果:糖尿病大鼠肾脏中PPARα和PPARδ蛋白表较正常对照大鼠明显降低(P＜0.01),PPARγ表达则较正常对照大鼠明显升高(P＜0.01).中高剂量小檗碱和非诺贝特都能促进糖尿病大鼠肾组织中PPARα和PPARδ的表达(P＜0.01),中高剂量小檗碱和罗格列酮能明显降低PPARγ表达(P＜0.01).结论:糖尿病大鼠肾脏组织中PPARα/δ/γ的表达失常,小檗碱能恢复其表达至接近正常大鼠水平.
목적:관찰2형당뇨통대서신조직PPARα/δ/γ단백적표체급소벽감대타문적영향.방법:소제량주사련뇨균소(35 mg·kg-1,ip)가고당고지사료사양16주건립2형당뇨병대서모형,수후16주매천분별급여저중고제량소벽감75、150、300mg·kg-1、비낙패특100mg·kg1 화라격렬동4mg·kg-1,처사대서후용면역조화기술검측신장조직중PPARα/δ/γ적표체.결과:당뇨병대서신장중PPARα화PPARδ단백표교정상대조대서명현강저(P＜0.01),PPARγ표체칙교정상대조대서명현승고(P＜0.01).중고제량소벽감화비낙패특도능촉진당뇨병대서신조직중PPARα화PPARδ적표체(P＜0.01),중고제량소벽감화라격렬동능명현강저PPARγ표체(P＜0.01).결론:당뇨병대서신장조직중PPARα/δ/γ적표체실상,소벽감능회복기표체지접근정상대서수평.
Objective To investigate the expression of peroxisome proliferator-activated receptors (PPARs) α/δ/γ protein and the effect of berberine on them in diabetic rat kidney.Methods Type 2 diabetic rats were induced by injection (ip) with streptozotocin 35 mg·kg1 and feeding with a high-carbohydrate/high-fat diet for 16 weeks.From week 17 to 32, diabetic rats were given low, middle, high-dose berberine 75, 150, 300 mg×kg-1, fenofibrate 100 mg×kg1 and rosiglitazone 4 mg×kg-1, respectively.The expression of PPARα/δ/γ protein in kidney were detected by immunohistochemistry.Results The expression of PPARα and PPARδ protein in the kidney of diabetic rat, which were significantly higher than that of the control one (P＜0.01), while PPARγ expression was obviously lower than that of the control one (P＜0.01).The expression of PPARα and PPARδ in in diabetic kidney were increased notably after middle-dose, high-dose berberine and fenofibrate treatment (P＜0.01);middle-dose, high-dose berberine and rosiglitazone treatment significantly reduced PPARγ expression (P＜0.01).Conclusion Berberine can restore the disturbed PPARα/δ/γ protein expression in diabetic rat kidney.