CAJ | 학술논문

目的探讨2,4-D和培养基对刺五加愈伤组织和体细胞胚胎生产刺五加苷B、E的影响,建立有利于刺五加苷B、E积累的刺五加体外培养体系.方法将来自同一外植体的刺五加愈伤组织和体细胞胚胎分别诱导增殖或产生次级体胚,HPLC法测定培养物中刺五加苷B、E含量和培养物质量,考察培养物类型、培养基种类和激素对刺五加苷B、E生产的影响.结果体细胞胚胎的质量虽略低于愈伤组织的质量,但体细胞胚胎生产刺五加苷B、E的能力优于愈伤组织.2,4-D对刺五加苷B、E含量影响较小,仅改变了培养物的质量.MS培养基处理中培养物的刺五加苷B、E产量优于1/2MS培养基.结论刺五加体外培养物可以生产刺五加苷B、E,其产量取决于培养物类型、培养基种类和2,4-D浓度.
목적 탐토2,4-D화배양기대자오가유상조직화체세포배태생산자오가감B、E적영향,건립유리우자오가감B、E적루적자오가체외배양체계.방법 장래자동일외식체적자오가유상조직화체세포배태분별유도증식혹산생차급체배,HPLC법측정배양물중자오가감B、E함량화배양물질량,고찰배양물류형、배양기충류화격소대자오가감B、E생산적영향.결과 체세포배태적질량수략저우유상조직적질량,단체세포배태생산자오가감B、E적능력우우유상조직.2,4-D대자오가감B、E함량영향교소,부개변료배양물적질량.MS배양기처리중배양물적자오가감B、E산량우우1/2MS배양기.결론 자오가체외배양물가이생산자오가감B、E,기산량취결우배양물류형、배양기충류화2,4-D농도.
Objective To discuss the effect of 2,4-D and culture medium to the callus and somatic embryos for the production of eleutherosides B, E, establish in vitro raise system of Eleutherococcus senticosus Harms.Methods Using Callus and somatic embryos which came from the same explant to induce multiplication or to produce secondary somatic embryos. Determined the content of eleutherosides B ,E and the weight of culture medium by HPLC. The effect of eleutherosides B ,E by the cultures type, the culture medium kind and the hormone were studied.Results Even though the weight of somatic embryos was slightly lower than that of the callus,the ability of somatic embryos to produce eleutherosides B, E was better than the callus. 2,4-D had little effect on eleutherosides B, E, and only changed the weight of cultures. The production of eleutherosides B, E came from the cultures which was dealt with MS medium was higher than that in 1/2 MS medium. Conclusion In vitro cultures of Eleutherococcus senticosus Harms can produce eleutherosides B, E, whose production is decided by the cultures type, the culture medium kind and the concentration of 2,4-D.