CAJ | 학술논문

采用因素轮换试验设计和均匀试验设计对现有枯草芽孢杆菌BE-91菌株的发酵工艺进行系统研究,获得木聚糖酶高产的优化发酵工艺为:国产胰蛋白胨1.5%,国产酵母膏0.05%,牛肉膏0.3%,KH_2PO_40.2%,MgSO_40.03%,NaCl 0.5%,吐温80 0.4%,起始pH 9.2,摇瓶装液量150 mL/500 mL,接种量3.5%,转速180 r/min,发酵温度37℃.在优化发酵工艺条件下培养BE-91菌株8 h,发酵液木聚糖酶活力达到423.24 U/mL,是优化前酶活力的1.5倍.BE-91菌株在国内已报道菌株中具有发酵周期短、耐热酸性木聚糖酶活力高的特点.
채용인소륜환시험설계화균균시험설계대현유고초아포간균BE-91균주적발효공예진행계통연구,획득목취당매고산적우화발효공예위:국산이단백동1.5%,국산효모고0.05%,우육고0.3%,KH_2PO_40.2%,MgSO_40.03%,NaCl 0.5%,토온80 0.4%,기시pH 9.2,요병장액량150 mL/500 mL,접충량3.5%,전속180 r/min,발효온도37℃.재우화발효공예조건하배양BE-91균주8 h,발효액목취당매활력체도423.24 U/mL,시우화전매활력적1.5배.BE-91균주재국내이보도균주중구유발효주기단、내열산성목취당매활력고적특점.
The fermentation process of high yield xylanase strain(Bacillus subtilis)BE-91 was optimized by One-Factor-at-a-Time Experimentation Method and Uniform Design Method. The optimal fermentation process were as follows : trypton (made in China) 1.5 % , yeast extract (made in China) 0.05% , beef powder 0.3% , KH_2PO_4 0.2% , MgSO_4 0.03% , NaCl 0.5% ,tween 80 0.4% ,initial pH 9.2, shake flask capacity 150 mL/500 mL, inoculure concentration 3.5%, stirring rate 180 r/min, temperature 37℃. Under the optimal fermentation process,xylanase activity was up to 423.24 U/mL after 8 h of cultivation, 1.5 times in comparison with the original fermentation process. So the strain BE-91 had advantages of short fermentation period and high thermostable acid xylanase activity in China.