CAJ | 학술논문

为研究芝芪菌质多糖体外免疫活性增强作用,本研究采用水提、醇沉、离子交换层析等方法纯化芝芪菌质多糖,并对各多糖组分(GAP-1、GAP-2、GAP-3、GAP-3)进行小鼠脾细胞增殖、NK细胞活性以及小鼠腹腔巨噬细胞吞噬鸡红细胞活性的测定.结果表明:纯化的4种芝芪菌质多糖在10~(-3)mg/mL～10~(-1)mg/mL浓度范围内均能够显著增强ConA诱导的小鼠脾淋巴细胞增殖反应;在25 mg/kg～100mg/kg剂量范围内均能够显著增强小鼠腹腔巨噬细胞吞噬鸡红细胞活性,而且呈剂量依赖性,其中以GAP-3效果最为明显;对NK细胞活性也有不同程度的增强作用,其中,GAP-3在浓度为100μg/mL时,NK细胞的杀伤率为27%,与IL-2阳性对照组比较,有显著增强作用(p<0.05).本研究证明,纯化的芝芪菌质多糖具有较好的体外增强免疫活性,为芝芪菌质作为增强机体免疫力的饲料添加剂提供了理论依据.
위연구지기균질다당체외면역활성증강작용,본연구채용수제、순침、리자교환층석등방법순화지기균질다당,병대각다당조분(GAP-1、GAP-2、GAP-3、GAP-3)진행소서비세포증식、NK세포활성이급소서복강거서세포탄서계홍세포활성적측정.결과표명:순화적4충지기균질다당재10~(-3)mg/mL～10~(-1)mg/mL농도범위내균능구현저증강ConA유도적소서비림파세포증식반응;재25 mg/kg～100mg/kg제량범위내균능구현저증강소서복강거서세포탄서계홍세포활성,이차정제량의뢰성,기중이GAP-3효과최위명현;대NK세포활성야유불동정도적증강작용,기중,GAP-3재농도위100μg/mL시,NK세포적살상솔위27%,여IL-2양성대조조비교,유현저증강작용(p<0.05).본연구증명,순화적지기균질다당구유교호적체외증강면역활성,위지기균질작위증강궤체면역력적사료첨가제제공료이론의거.
The enhancing immunoactivity of the Zhiqi (Ganoderma Iucidum ＆ Astragalus membranaceus) fungal polysacc-harides was investigated in mice. Four portions polysaccharides were tested and shown significant enhancement to ConA-induced spleen lymphocyte proliferation response and mouse peritoneal macrophage phagocytic activity to chicken erythrocyte. The NK cell activities were also enhanced, where the GAP-3 was shown to promote NK cell-killing rate up to 27 % at 100 μg/mL dose. Theseresults provide an experimental basis for Zhiqi fungal substance as a feed additive to improve the animal immunity.