北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
JOURNAL OF BEIJING MEDICAL UNIVERSITY(HEALTH SCIENCES)
2010年
2期
188-191
,共4页
和清源%韩鸿宾%许方婧伟%闫军浩%曾津津%李小刚%傅瑜%彭芸%陈翯%侯超%徐晓娟
和清源%韓鴻賓%許方婧偉%閆軍浩%曾津津%李小剛%傅瑜%彭蕓%陳翯%侯超%徐曉娟
화청원%한홍빈%허방청위%염군호%증진진%리소강%부유%팽예%진학%후초%서효연
磁共振成像%细胞外隙%钆喷酸葡胺
磁共振成像%細胞外隙%釓噴痠葡胺
자공진성상%세포외극%구분산포알
Magnetic resonance imaging%Extracellular space%Gadolinium DTPA
目的:观察钆喷酸葡胺(Gd-DTPA)在脑细胞外间隙(brain extracellular space,ECS)中扩散的磁共振成像(magnetic resonance imaging,MRI)表现与规律,探讨应用MRI示踪法对ECS进行定量分析的可行性.方法:将8只雄性SD大鼠(280 g～320 g)麻醉后,参考脑立体定位图谱,选取冠状位苍白球为中心层面,以尾状核为靶点进行穿刺定位,导入Gd-DTPA 2 μL.于导入后1、3、6、9、12 h分别进行MRI扫描,观察MRI表现,测量示踪剂导入侧所在脑区与对侧相应脑区MR信号强度随时间的变化规律.于12 h MRI扫描结束后,对大鼠进行行为学评分.结果:MRI图像证实6只大鼠Gd-DTPA导入点位于尾状核区,2只位于外囊区.Gd-DTPA在尾状核区导入后呈均匀扩散,3 h扩散到达同侧大脑皮层,扩散后主要分布在大脑中动脉供血区的脑组织.随着扩散范围的增大,尾状核区的MRI信号强度逐渐下降,3 h后各个时间点相对MRI信号强度△s与1 h时△s间差异存在统计学意义(t=95.63,P<0.01),3 h后的各组△S间差异无统计学意义(P>0.05).△s的衰减规律呈指数形式[Y=1.7886x~(-0.1776)(R~2=0.94)].导入外囊区的Gd-DTPA在1 h的MRI上呈弧形沿自质纤维扩散,表现为各向异性.在3 h后呈扇形向相邻脑区扩散,6 h时扩散到达同侧皮层区.结论:应用MRI示踪法可动态观察Gd-DTPA在细胞外间隙的扩散与清除衰减规律,是在活体对ECS解剖与生理学参数进行定量研究的可行方法.
目的:觀察釓噴痠葡胺(Gd-DTPA)在腦細胞外間隙(brain extracellular space,ECS)中擴散的磁共振成像(magnetic resonance imaging,MRI)錶現與規律,探討應用MRI示蹤法對ECS進行定量分析的可行性.方法:將8隻雄性SD大鼠(280 g～320 g)痳醉後,參攷腦立體定位圖譜,選取冠狀位蒼白毬為中心層麵,以尾狀覈為靶點進行穿刺定位,導入Gd-DTPA 2 μL.于導入後1、3、6、9、12 h分彆進行MRI掃描,觀察MRI錶現,測量示蹤劑導入側所在腦區與對側相應腦區MR信號彊度隨時間的變化規律.于12 h MRI掃描結束後,對大鼠進行行為學評分.結果:MRI圖像證實6隻大鼠Gd-DTPA導入點位于尾狀覈區,2隻位于外囊區.Gd-DTPA在尾狀覈區導入後呈均勻擴散,3 h擴散到達同側大腦皮層,擴散後主要分佈在大腦中動脈供血區的腦組織.隨著擴散範圍的增大,尾狀覈區的MRI信號彊度逐漸下降,3 h後各箇時間點相對MRI信號彊度△s與1 h時△s間差異存在統計學意義(t=95.63,P<0.01),3 h後的各組△S間差異無統計學意義(P>0.05).△s的衰減規律呈指數形式[Y=1.7886x~(-0.1776)(R~2=0.94)].導入外囊區的Gd-DTPA在1 h的MRI上呈弧形沿自質纖維擴散,錶現為各嚮異性.在3 h後呈扇形嚮相鄰腦區擴散,6 h時擴散到達同側皮層區.結論:應用MRI示蹤法可動態觀察Gd-DTPA在細胞外間隙的擴散與清除衰減規律,是在活體對ECS解剖與生理學參數進行定量研究的可行方法.
목적:관찰구분산포알(Gd-DTPA)재뇌세포외간극(brain extracellular space,ECS)중확산적자공진성상(magnetic resonance imaging,MRI)표현여규률,탐토응용MRI시종법대ECS진행정량분석적가행성.방법:장8지웅성SD대서(280 g～320 g)마취후,삼고뇌입체정위도보,선취관상위창백구위중심층면,이미상핵위파점진행천자정위,도입Gd-DTPA 2 μL.우도입후1、3、6、9、12 h분별진행MRI소묘,관찰MRI표현,측량시종제도입측소재뇌구여대측상응뇌구MR신호강도수시간적변화규률.우12 h MRI소묘결속후,대대서진행행위학평분.결과:MRI도상증실6지대서Gd-DTPA도입점위우미상핵구,2지위우외낭구.Gd-DTPA재미상핵구도입후정균균확산,3 h확산도체동측대뇌피층,확산후주요분포재대뇌중동맥공혈구적뇌조직.수착확산범위적증대,미상핵구적MRI신호강도축점하강,3 h후각개시간점상대MRI신호강도△s여1 h시△s간차이존재통계학의의(t=95.63,P<0.01),3 h후적각조△S간차이무통계학의의(P>0.05).△s적쇠감규률정지수형식[Y=1.7886x~(-0.1776)(R~2=0.94)].도입외낭구적Gd-DTPA재1 h적MRI상정호형연자질섬유확산,표현위각향이성.재3 h후정선형향상린뇌구확산,6 h시확산도체동측피층구.결론:응용MRI시종법가동태관찰Gd-DTPA재세포외간극적확산여청제쇠감규률,시재활체대ECS해부여생이학삼수진행정량연구적가행방법.
Objective:To observe the diffusion of Gd-DTPA in brain extracellular space (ECS) by magnetic resonance imaging (MRI) and investigate the feasibility of ECS measurement by using MRI tra cer method in vivo.Methods:2 μL Gd-DTPA was introduced into ECS by caudate nucleus according to stereotaxic atlas in 8 Sprague Dawley (SD) rats (male,280～320 g).The MRI scans were performed at 1 h,3 h,6 h,9 h and 12 h respectively after administration.MRI appearances of Gd-DTPA diffusion and distribution was observed and compared.The MRI signal enhancement was measured at each time point.The neuroethology assessment was performed after MRI scanning at 12 h.Results:The injection was accurate at the center of the caudate nucleus in 6 rats,while,at the capsula externa in other 2 rats.Gd-DTPA diffused isotropically after it was introduced into caudate nucleus,which spread into lateral cor-tex at 3 h.The MRI signal enhancement distributed mainly in the middle cerebral artery territory.A sig-nificant difference was found between the signal enhancement ratio at 1 h and that at 3 h in the original point of caudate nucleus(t=95.63,P<0.01),and the signal enhancement attenuated following the ex-ponential power function y=1.7886x~(-0.1776) (R~2=0.94).In 2 rats with the injection point at capsula ex-terna,Gd-DTPA diffused anisotropically along the fiber track of white matter during 1 h to 3 h,and spread into the lateral cortex at 6 h.Conclusion:The diffusion and clearance of Gd-DTPA in brain ECS could be monitored and measured quantitatively in vivo by MRI tracer method.
