CAJ | 학술논문

[目的]建立颠茄高频再生体系及筛选卡那霉素(Kan)抗性.[方法]以颠茄叶片及腋芽为外植体,研究了培养基中6-BA和NAA不同配比对其不定芽分化的影响以及叶片不定芽对Kan的敏感性.[结果]MS+4.5 mg/L6-BA+0.2 mg/L NAA为叶片不定芽分化的最佳培养基,不定芽分化率达100%,在1.0 cm×1.0 cm叶块上的不定芽分化数平均达5.85;MS+3.0mg/L 6-BA+0.1 mg/L NAA为腋芽不定芽分化的最适培养基,不定芽分化率达100%,每个腋芽不定芽分化平均数为4～8个;400.0 mg/L Kan为颠茄叶片遗传转化的最佳筛选浓度.[结论]为颠茄无菌苗的快速繁殖以及基于叶盘法的遗传转化奠定了基础.
[목적]건립전가고빈재생체계급사선잡나매소(Kan)항성.[방법]이전가협편급액아위외식체,연구료배양기중6-BA화NAA불동배비대기불정아분화적영향이급협편불정아대Kan적민감성.[결과]MS+4.5 mg/L6-BA+0.2 mg/L NAA위협편불정아분화적최가배양기,불정아분화솔체100%,재1.0 cm×1.0 cm협괴상적불정아분화수평균체5.85;MS+3.0mg/L 6-BA+0.1 mg/L NAA위액아불정아분화적최괄배양기,불정아분화솔체100%,매개액아불정아분화평균수위4～8개;400.0 mg/L Kan위전가협편유전전화적최가사선농도.[결론]위전가무균묘적쾌속번식이급기우협반법적유전전화전정료기출.
[Objective] The research aimed to establish the high frequency regeneration system of Atropa belladonna L. and screen the kanamycin (Kan) resistance. [Method] The leaves and axillary buds of Atropa belladonna L. were as the explants, the influences of different ratios of 6-BA and NAA in the medium on the adventitious bud differentiation and the sensibility of leaf on Kan were studied. [Result] MS +4.5 mg/L6-BA +0.2 mg/L NAA was the optimum medium of leaf adventitious bud differentiation, and the differentiation ratio of adventitious bud reached 100%. The number of adventitious bud differentiation on 1.0cmx1.0cm leaf block averagely reached 5.85. MS +3.0 mg/L 6-BA +0.1 mg/L NAA was the optimum medium of axillary bud adventitious bud differentiation, and the differentiation ratio of adventitious bud reached 100%. The average number of adventitious bud differentiation in every axillary bud was during 4 -8. The optimum screening concentration for genetic transformation of Atropa belladonna L. leaf was 400.0 mg/L of Kan. [Conclusion] The research laid the foundation for the rapid propagation of Atropa belladonna L. aseptic seedling and the genetic transformation based on the leaf disc cocultivation.