国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2011年
2期
158-160
,共3页
肿瘤,基底细胞%S期激酶相关蛋白质类%周期素依赖激酶抑制剂p27
腫瘤,基底細胞%S期激酶相關蛋白質類%週期素依賴激酶抑製劑p27
종류,기저세포%S기격매상관단백질류%주기소의뢰격매억제제p27
Neoplasms,basal cell%S-phase kinase-associated proteins%Cyclin-dependent kinase inhibitor p27
目的 检测S期激酶相关蛋白2(Skp2)与激酶抑制蛋白1(P27kip1)在基底细胞癌(BCC)中的表达,进而探明两者在BCC发生发展中的作用及其相关性,以及与临床病理特征的关系,并初步探讨Skp2与P27kip1在BCC 临床分型中的作用,尤其在硬化型中的作用.方法用免疫组织染色SP两步法检测50例BCC及30例正常皮肤组织中Skp2与P27kip1的表达情况,并结合患者的年龄、性别及临床分型等进行综合分析.结果 Skp2定位于胞核或胞质,而P27kip1只定位于胞核.Skp2在BCC中呈高表达,而P27kip1呈低表达,与正常皮肤组织对照组比较,差异有统计学意义(P＜0.05).Skp2和P27kip1的异常表达与BCC的临床分型有关(P＜0.05),而与患者性别、年龄等因素均无关(P＞0.05).在BCC中,Skp2的阳性表达与P27kip1的阳性表达呈负相关(r=-0.624;P＜0.05);但在BCC的硬化型中,两者无相关性(P＞0.05).结论 Skp2与P27kip1在BCC中的阳性表达呈负相关,提示在BCC的发生及发展中两者可能起协同作用,并可能为将来的治疗提供靶点.Skp2在硬化型BCC组的表达高于结节型及浅表型BCC组,而P27kip1则相反,提示Skp2与P27kip1可能参与皮肤BCC的分型,且可能成为BCC分型的预测因子,从而为不同类型BCC手术切缘的设计提供生化指标依据.
目的 檢測S期激酶相關蛋白2(Skp2)與激酶抑製蛋白1(P27kip1)在基底細胞癌(BCC)中的錶達,進而探明兩者在BCC髮生髮展中的作用及其相關性,以及與臨床病理特徵的關繫,併初步探討Skp2與P27kip1在BCC 臨床分型中的作用,尤其在硬化型中的作用.方法用免疫組織染色SP兩步法檢測50例BCC及30例正常皮膚組織中Skp2與P27kip1的錶達情況,併結閤患者的年齡、性彆及臨床分型等進行綜閤分析.結果 Skp2定位于胞覈或胞質,而P27kip1隻定位于胞覈.Skp2在BCC中呈高錶達,而P27kip1呈低錶達,與正常皮膚組織對照組比較,差異有統計學意義(P＜0.05).Skp2和P27kip1的異常錶達與BCC的臨床分型有關(P＜0.05),而與患者性彆、年齡等因素均無關(P＞0.05).在BCC中,Skp2的暘性錶達與P27kip1的暘性錶達呈負相關(r=-0.624;P＜0.05);但在BCC的硬化型中,兩者無相關性(P＞0.05).結論 Skp2與P27kip1在BCC中的暘性錶達呈負相關,提示在BCC的髮生及髮展中兩者可能起協同作用,併可能為將來的治療提供靶點.Skp2在硬化型BCC組的錶達高于結節型及淺錶型BCC組,而P27kip1則相反,提示Skp2與P27kip1可能參與皮膚BCC的分型,且可能成為BCC分型的預測因子,從而為不同類型BCC手術切緣的設計提供生化指標依據.
목적 검측S기격매상관단백2(Skp2)여격매억제단백1(P27kip1)재기저세포암(BCC)중적표체,진이탐명량자재BCC발생발전중적작용급기상관성,이급여림상병리특정적관계,병초보탐토Skp2여P27kip1재BCC 림상분형중적작용,우기재경화형중적작용.방법용면역조직염색SP량보법검측50례BCC급30례정상피부조직중Skp2여P27kip1적표체정황,병결합환자적년령、성별급림상분형등진행종합분석.결과 Skp2정위우포핵혹포질,이P27kip1지정위우포핵.Skp2재BCC중정고표체,이P27kip1정저표체,여정상피부조직대조조비교,차이유통계학의의(P＜0.05).Skp2화P27kip1적이상표체여BCC적림상분형유관(P＜0.05),이여환자성별、년령등인소균무관(P＞0.05).재BCC중,Skp2적양성표체여P27kip1적양성표체정부상관(r=-0.624;P＜0.05);단재BCC적경화형중,량자무상관성(P＞0.05).결론 Skp2여P27kip1재BCC중적양성표체정부상관,제시재BCC적발생급발전중량자가능기협동작용,병가능위장래적치료제공파점.Skp2재경화형BCC조적표체고우결절형급천표형BCC조,이P27kip1칙상반,제시Skp2여P27kip1가능삼여피부BCC적분형,차가능성위BCC분형적예측인자,종이위불동류형BCC수술절연적설계제공생화지표의거.
Objective To investigate the expression of Skp2 and P27kip1 and their relationship with the clinicopathologic characters in basal cell carcinoma(BCC). To explore their expression between with the types of BCC of skin, especially in morpheaform BCC group. Methods Immunohistochemical SP technique was used to examine the expression of Skp2 and P27kip1 in 50 cases of BCC and 30 cases of normal tissues of skin.The expression level was analyzed combined with clinicopathological features of age, sex and types of tumor.Results Skp2 positive products located in cytoplasm or nucleus. P27kip1 positive products located in nucleus.The high expression of Skp2 and the low expression of P27kip1 were observed in BCC, there was statistical significance between the tumor group and the control group (P ＜ 0. 05 ) . The expression of Skp2 and P27kip1 were correlated with clinical types of BCC ( P ＜ 0. 05 ), while which were not correlated with age and sex ( P ＞0.05 ). In BCC tissue, Skp2 expression was negatively correlated with P27kip1 ( - 0.624; P ＜ 0.05), there was no correlated with each other in morpheaform BCC group ( P ＞ 0. 05 ). Conclusion The expression of Skp2 and P27kip1 in BCC are significant differences to normal skin tissues, and they are correlated with each other.They may play an important role in carcinogenesis and the development of BCC, and also make targets to treat it. Compared to nodular and superficial BCC group, the expression of Skp2 are higher in morpheaform BCC group, and the expression of P27kip1 is lower. They may play an important role in the types of the BCC and useful factors to predict the types of the tumor, also determine surgical margin for BCC cases.
