CAJ | 학술논문

目的评价经肋间后动脉移植骨髓基质干细胞(BMSCs)修复创伤性脊髓损伤的可行性. 方法选取2只家兔提取BMSCs,扩大培养后采用菲立磁标记及绿色荧光蛋白基因转染.另选取20只家兔随机分为两组:经肋间后动脉注射BMSCs组和只创建脊髓损伤模型组.结果观察包括:BMSCs的核磁共振示踪,脊髓标本宏观及微观观察,神经电生理检查. 结果实验第14天内,磁共振显示BMSCs逐渐迁移到脊髓损伤区域,并经脊髓组织切片荧光显微镜检查进一步得到证实；脊髓组织切片电镜检查发现实验组可见大量新生髓鞘,部分髓鞘的少突胶质细胞的细胞质内含有菲立磁颗粒；神经电生理结果显示:第7天时实验组与对照组损伤脊髓节段神经传导功能比较差异无统计学意义(P＞0.05),第14天时两组比较差异有统计学意义(P＜0.05). 结论经肋间后动脉介入移植BMSCs修复脊髓损伤是一种可重复、高效的干细胞移植途径.
목적 평개경륵간후동맥이식골수기질간세포(BMSCs)수복창상성척수손상적가행성. 방법 선취2지가토제취BMSCs,확대배양후채용비립자표기급록색형광단백기인전염.령선취20지가토수궤분위량조:경륵간후동맥주사BMSCs조화지창건척수손상모형조.결과관찰포괄:BMSCs적핵자공진시종,척수표본굉관급미관관찰,신경전생리검사. 결과 실험제14천내,자공진현시BMSCs축점천이도척수손상구역,병경척수조직절편형광현미경검사진일보득도증실；척수조직절편전경검사발현실험조가견대량신생수초,부분수초적소돌효질세포적세포질내함유비립자과립；신경전생리결과현시:제7천시실험조여대조조손상척수절단신경전도공능비교차이무통계학의의(P＞0.05),제14천시량조비교차이유통계학의의(P＜0.05). 결론 경륵간후동맥개입이식BMSCs수복척수손상시일충가중복、고효적간세포이식도경.
Objective To evaluate the feasibility of transplantation of bone marrow stromal cells (BMSCs) via posterior intercostal arteries for the treatment of spinal cord injury (SCI). Methods BMSCs which had been obtained from 2 rabbits were expanded after intermediate culture.All BMSCs were labeled with Feridex and transfected by green fluorescent protein (GFP).Another 20 rabbits of SCI models were randomized into 2 groups:a BMSCs group in which BMSCs were injected into the posterior intercostal arteries and a control group in which no treatment was adopted.The injected BMSCs were monitored by MRI in vivo.Fluorescence images and electron micrographs of the spinal cord were taken to confirm the MRI results and nerve conduction of the damaged spinal cord was asessed by nerve electrophysiological examination.On 7 and 14 days post-experiment,the latency and amplitude of motion evoked potential (MEP) in 2 groups was measured. Results MRI showed migration of BMSCs to the injury sites 14 days post-transplantation,which was confirmed by fluorescent microscpy.The myelin regenerated by oligodendrocytes was shown containing Fetidex particles as observed by electron microscopy.There was no significant difference between the 2 groups on day 7 in nerve conduction of the damaged spinal cord ( P ＞ 0.05).On the 14 day,however,the MEP latency and amplitude in the control group and the experiment group were 26.7 ± 0.43 vs.17.9 ± 0.46 ms ( n =10) and 39.9 ± 0.65 vs.43.3 ± 0.67μv ( n =10) respectively,with significant differences between the 2 groups (P ＜0.05). Conclusion Transplanting BMSCs via postetior intercostal arteries is a noninvasive and efficient intervention for treatment of SCI,available for clinical experiment.