中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
3期
265-269
,共5页
游华%矫树生%冯帅南%陈建梅%李兵仓
遊華%矯樹生%馮帥南%陳建梅%李兵倉
유화%교수생%풍수남%진건매%리병창
创伤和损伤%周围神经%组织工程
創傷和損傷%週圍神經%組織工程
창상화손상%주위신경%조직공정
Wounds and injures%Peripheral nerves%Tissue engineering
目的 研究嗅球成鞘细胞(olfactory ensheathing cell,OEC)-雪旺细胞(Schwann cell,SC)-细胞外基质成分(extracellular matrix,ECM)-聚乳酸-聚羟基乙酸共聚物[poly(DLlactide-co-glycolide acid),PLGA]桥接体对大鼠坐骨神经缺损后外周靶器官及脊髓神经元的保护作用.方法 建立SD大鼠右侧坐骨神经15 mm缺损模型后,用OEC、SC、ECM以及自行制备的PLGA导管,构成OEC-SC-ECM-PLGA桥接体进行修复,同时设OEC-ECM-PLGA组、SC-ECM-PLGA组、ECM-PLGA组、PLGA组和自体神经移植对照组.在术后1,3,6,9周行腓肠肌湿重恢复率及运动终板检测,并于术后9周行CM-Dil和霍乱毒素B亚单位-辣根过氧化物酶(CB-HRP)逆行示踪检测.结果 术后各组动物术侧腓肠肌湿莺和运动终板数量下降,3周后除ECM-PLGA组和PLGA组外,腓肠肌湿重和运动终板数量逐渐增加.9周时OEC-SC-ECM-PLGA组腓肠肌湿重、运动终板数量及运动终板长轴长度与自体神经移植组差异无统计学意义(P>0.05).9周时CM-DiI和CB-HRP逆行示踪结果显示,OEC-SC-ECM-PLGA组标记的脊髓前角运动神经元数量与自体神经移植组差异无统计学意义(P>0.05).结论 OEC-SC-ECM-PLGA桥接体对大鼠坐骨神经缺损后的外周靶器官及脊髓神经元具有一定的保护作用.
目的 研究嗅毬成鞘細胞(olfactory ensheathing cell,OEC)-雪旺細胞(Schwann cell,SC)-細胞外基質成分(extracellular matrix,ECM)-聚乳痠-聚羥基乙痠共聚物[poly(DLlactide-co-glycolide acid),PLGA]橋接體對大鼠坐骨神經缺損後外週靶器官及脊髓神經元的保護作用.方法 建立SD大鼠右側坐骨神經15 mm缺損模型後,用OEC、SC、ECM以及自行製備的PLGA導管,構成OEC-SC-ECM-PLGA橋接體進行脩複,同時設OEC-ECM-PLGA組、SC-ECM-PLGA組、ECM-PLGA組、PLGA組和自體神經移植對照組.在術後1,3,6,9週行腓腸肌濕重恢複率及運動終闆檢測,併于術後9週行CM-Dil和霍亂毒素B亞單位-辣根過氧化物酶(CB-HRP)逆行示蹤檢測.結果 術後各組動物術側腓腸肌濕鶯和運動終闆數量下降,3週後除ECM-PLGA組和PLGA組外,腓腸肌濕重和運動終闆數量逐漸增加.9週時OEC-SC-ECM-PLGA組腓腸肌濕重、運動終闆數量及運動終闆長軸長度與自體神經移植組差異無統計學意義(P>0.05).9週時CM-DiI和CB-HRP逆行示蹤結果顯示,OEC-SC-ECM-PLGA組標記的脊髓前角運動神經元數量與自體神經移植組差異無統計學意義(P>0.05).結論 OEC-SC-ECM-PLGA橋接體對大鼠坐骨神經缺損後的外週靶器官及脊髓神經元具有一定的保護作用.
목적 연구후구성초세포(olfactory ensheathing cell,OEC)-설왕세포(Schwann cell,SC)-세포외기질성분(extracellular matrix,ECM)-취유산-취간기을산공취물[poly(DLlactide-co-glycolide acid),PLGA]교접체대대서좌골신경결손후외주파기관급척수신경원적보호작용.방법 건립SD대서우측좌골신경15 mm결손모형후,용OEC、SC、ECM이급자행제비적PLGA도관,구성OEC-SC-ECM-PLGA교접체진행수복,동시설OEC-ECM-PLGA조、SC-ECM-PLGA조、ECM-PLGA조、PLGA조화자체신경이식대조조.재술후1,3,6,9주행비장기습중회복솔급운동종판검측,병우술후9주행CM-Dil화곽란독소B아단위-랄근과양화물매(CB-HRP)역행시종검측.결과 술후각조동물술측비장기습앵화운동종판수량하강,3주후제ECM-PLGA조화PLGA조외,비장기습중화운동종판수량축점증가.9주시OEC-SC-ECM-PLGA조비장기습중、운동종판수량급운동종판장축장도여자체신경이식조차이무통계학의의(P>0.05).9주시CM-DiI화CB-HRP역행시종결과현시,OEC-SC-ECM-PLGA조표기적척수전각운동신경원수량여자체신경이식조차이무통계학의의(P>0.05).결론 OEC-SC-ECM-PLGA교접체대대서좌골신경결손후적외주파기관급척수신경원구유일정적보호작용.
Objective To explore the protective effect of olfactory ensheathing cell(OEC)Schwann cell(SC)-extracellular matrix(ECM)-poly(DL-lactide-co-glycolide acid)(PLGA)bridging complex on peripheral target organ and spinal cord neurons after rat sciatic nerve defect.Methods A 15 mm right sciatic nerve defect model was established in SD rats and repaired with OEC-SC-ECM-PLGA bridging complex that contained OEC,SC,ECM and self-made PLGA conduit.At the same time,the study set OEC-ECM-PLGA group,SC-ECM-PLGA group,ECM-PLGA group,PLGA group and nerve autograft control group.At 1,3,6 and 9 weeks after surgery,the gastrocnemius muscle water weight test and motor end-plate test were performed.At the 9th week after surgery,CM-DiI and horseradish peroxidase(HRP)retrograde tracing were performed.Results The gastrocnemius muscle water weight and number of motor end-plate were decreased in all groups after surgery but gradually increased after three weeks except for ECM-PLGA group and PLGA group.At the 9th week,OEC-SC-ECM-PLGA group showed no statistical differences with nerve autograft group in aspects of gastrocnemius muscle water weight,number of motor end-plate and length of motor end-plate major axis(P > 0.05).At the 9th week,CM-DiI and HRP retrograde tracing found that the number of positive neurons in spinal cord in OEC-SC-ECM-PLGA group showed no statistical differences compared with nerve autograft group(P >0.05).Conclusions OEC-SC-ECM-PLGA bridging complex can partially protect peripheral target organ and spinal cord neurons after rat sciatic nerve defect.