CAJ | 학술논문

目的探讨靶向超声介导VEGF_(165) cDNA、抗ICAM-1单克隆抗体及两者协同作用于受损心肌的有效性及可行性.方法将80只心肌缺血的雄性SD大鼠随机分为四组:第一组采用超声击破微泡造影剂的方法将VEGF_(165) cDNA基因及抗ICAM-1单克隆抗体混合物定向于受损心肌;第二组采用超声击破微泡造影剂的方法将VEGF_(165) cDNA基因定向于受损心肌;第三组采用超声击破微泡造影剂的方法将抗ICAM-1单克隆抗体定向于受损心肌;第四组为对照组只注射生理盐水1 ml,不采用超声波照射.每组分别于24 h和14 d后处死10只.处死后分别行坏死区中性粒细胞记数,梗死区测定,免疫组织化学检测缺血心肌中的VEGF蛋白表达,缺血坏死心肌区域毛细血管记数.结果第一组大鼠心肌缺血治疗效果较第二、第三组效果显著,第一组中性粒细胞浸润以及心肌梗死区域均明显低于其他各组,VEGF蛋白表达以及毛细血管记数明显高于其他三组.结论采用超声触发破坏含靶基因及抗体的微泡造影剂的方式,可明显对抗心肌再损伤,促进缺血心肌的新生血管再生,改善心肌缺血,且两者协同作用比单独作用效果更佳.
목적 탐토파향초성개도VEGF_(165) cDNA、항ICAM-1단극륭항체급량자협동작용우수손심기적유효성급가행성.방법 장80지심기결혈적웅성SD대서수궤분위사조:제일조채용초성격파미포조영제적방법장VEGF_(165) cDNA기인급항ICAM-1단극륭항체혼합물정향우수손심기;제이조채용초성격파미포조영제적방법장VEGF_(165) cDNA기인정향우수손심기;제삼조채용초성격파미포조영제적방법장항ICAM-1단극륭항체정향우수손심기;제사조위대조조지주사생리염수1 ml,불채용초성파조사.매조분별우24 h화14 d후처사10지.처사후분별행배사구중성립세포기수,경사구측정,면역조직화학검측결혈심기중적VEGF단백표체,결혈배사심기구역모세혈관기수.결과 제일조대서심기결혈치료효과교제이、제삼조효과현저,제일조중성립세포침윤이급심기경사구역균명현저우기타각조,VEGF단백표체이급모세혈관기수명현고우기타삼조.결론 채용초성촉발파배함파기인급항체적미포조영제적방식,가명현대항심기재손상,촉진결혈심기적신생혈관재생,개선심기결혈,차량자협동작용비단독작용효과경가.
Objective To study the cardioprotective effect of VEGF_(165) cDNA gene and monoclonal antibody to interceller adhesion molecule-1(ICAM-1)delivering and two coordination to myocardial ischemia-reperfusion injury of rats by ultrasound targeted microbubble destructive.Methods Eighty male SD rat models of myocardial ischemia-reperfusion injury(I/R)were divided into 4 groups.Using microbubble contrast agent destructive methods,the first group was infused VEGF_(165) cDNA and ICAM-1,the second group was infused VEGF_(165) cDNA,the third group was infused ICAM-1,the fourth group was used as control group.Each group was divided into two groups again.One group(24 h group)was killed after 24 hours and another group(14 d group)was killed after 14day,the expression of VEGF protein was accessed by immunohistochemical technique.Microvascular density(MVD)and polymorphonuclears(PMN)were counted in the infarcted area.The area of myocardial necrosis was measured.Results The infiltration of PMN and the area of myocardial necrosis received ultrasound-mediated in the 24 h group of the first group was lower than other groups.VEGF protein expression and MVD in 14 d group of the first group was higher than other groups.Conclusions Using targeted ultrasound may enhance obviously concentration of VEGF_(165) cDNA gene and ICAM-1 in myocardial ischemia.And the synergy of VEGF16s cDNA gene and ICAM-1 is better than the single.