CAJ | 학술논문

将大鼠分为空白对照组、经典组和骨碎补组,采用全骨髓贴壁法分离纯化大鼠骨髓间充质干细胞(BMSCs),检测BMSCs成骨分化过程碱性磷酸酶活性、矿化结节、转化生长因子-β1(TGF-β1)和骨形态发生蛋白2(BMP-2)的分泌.结果显示,骨碎补总黄酮促进成骨分化同时增加TGF-β1、BMP-2分泌(均P＜0.05).骨碎补总黄酮可能通过上调二者的表达促进成骨,对骨质疏松症可能有积极的治疗意义.
장대서분위공백대조조、경전조화골쇄보조,채용전골수첩벽법분리순화대서골수간충질간세포(BMSCs),검측BMSCs성골분화과정감성린산매활성、광화결절、전화생장인자-β1(TGF-β1)화골형태발생단백2(BMP-2)적분비.결과현시,골쇄보총황동촉진성골분화동시증가TGF-β1、BMP-2분비(균P＜0.05).골쇄보총황동가능통과상조이자적표체촉진성골,대골질소송증가능유적겁적치료의의.
The rats were assigned to blank control group, classical induction group, and drynaria total flavonoid group. Whole bone marrow culture method was applied to isolate and purify rats bone mesenchymal stem cells (BMSCs). Akaline phosphatase activity, calcium nodes, TGF-β1 and BMP-2 secretion in the process of bone mesenchymal stem cells osteogenic differentiation were detected. The results showed that compared to the blank group and classical group, drynaria total flavonoid promoted osteogenic differentiation accompanied with increased TGF-β1 and BMP-2 secretion (all P＜0. 05). Drynaria total flavonoid may promote osteogenic differentiation of BMSCs via upregulating TGF-β1 and BMP-2 expressions, and play an active role in the treatment of osteoporosis.