CAJ | 학술논문

目的建立大鼠终板软骨细胞体外自然传代的退变模型,探讨自然退变过程中内源性转化生长因子(TGF)-β1与钙化基因表达变化的关系.方法取大鼠腰椎终板软骨细胞,酶消化法及自然传代法分离培养大鼠终板软骨细胞,选取P2代和P4代,在体外均培养6 d,观察细胞表型及利用茜素红染色,观察P2与P4代细胞的变化.用实时PCR检测软骨标志基因Ⅱ型胶原、转录因子SOX-9及代谢相关基因蛋白多糖、基质金属蛋白酶(MMP)-13、含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(ADAMTD)4、5的变化,验证体外退变模型的建立,在此基础上,继续检测生长因子TGF-β1及钙化相关基因(ANK)、胞外核苷酸焦磷酸酶(ENPP)、组织非特异性碱性磷酸酶(TNAP)的变化.结果相对于P2代细胞,P4代细胞形态上有梭形变趋势,茜素红染色无明显变化.P4代转录因子SOX-9表达明显低于P2代(P4/P2=0.0690,P=0.0489),Ⅱ型胶原(P4/P2=0.0535,P=0.009)及蛋白多糖(P4/P2=0.2672,P=0.0343)表达也均明显低于P2代,其他代谢相关基因无明显改变.P4代细胞TGF-β1(P4/P2=0.5934,P=0.0482)、TNAP(P4/P2=0.0385,P=0.0139)和ANK(P4/P2=0.2121,P=0.0009)表达均明显低于P2代,ENPP表达无明显改变(P＞0.05).结论终板软骨随着传代次数增多,从P2代到P4代,开始发生体外自然退变,Ⅱ型胶原、SOX-9及蛋白多糖明显下调.内源性TGF-β1与钙化相关基因表达均下调,钙化相关基因ANK下调可能由内源性TGF-β1下调引起,提示调节内源性TGF-β1在终板软骨中的表达有可能会阻止椎间盘的退变.
목적 건립대서종판연골세포체외자연전대적퇴변모형,탐토자연퇴변과정중내원성전화생장인자(TGF)-β1여개화기인표체변화적관계.방법 취대서요추종판연골세포,매소화법급자연전대법분리배양대서종판연골세포,선취P2대화P4대,재체외균배양6 d,관찰세포표형급이용천소홍염색,관찰P2여P4대세포적변화.용실시PCR검측연골표지기인Ⅱ형효원、전록인자SOX-9급대사상관기인단백다당、기질금속단백매(MMP)-13、함Ⅰ형혈소판결합단백기서적해취단백양금속단백매(ADAMTD)4、5적변화,험증체외퇴변모형적건립,재차기출상,계속검측생장인자TGF-β1급개화상관기인(ANK)、포외핵감산초린산매(ENPP)、조직비특이성감성린산매(TNAP)적변화.결과 상대우P2대세포,P4대세포형태상유사형변추세,천소홍염색무명현변화.P4대전록인자SOX-9표체명현저우P2대(P4/P2=0.0690,P=0.0489),Ⅱ형효원(P4/P2=0.0535,P=0.009)급단백다당(P4/P2=0.2672,P=0.0343)표체야균명현저우P2대,기타대사상관기인무명현개변.P4대세포TGF-β1(P4/P2=0.5934,P=0.0482)、TNAP(P4/P2=0.0385,P=0.0139)화ANK(P4/P2=0.2121,P=0.0009)표체균명현저우P2대,ENPP표체무명현개변(P＞0.05).결론 종판연골수착전대차수증다,종P2대도P4대,개시발생체외자연퇴변,Ⅱ형효원、SOX-9급단백다당명현하조.내원성TGF-β1여개화상관기인표체균하조,개화상관기인ANK하조가능유내원성TGF-β1하조인기,제시조절내원성TGF-β1재종판연골중적표체유가능회조지추간반적퇴변.
Objective To explore the relationship between endogenous transforming growth factor (TGF) -β1 and calcification-related genes through an in vitro degeneration model by propagating rat endplate chondrocytes during a natural degeneration process. Methods Endplate chondrocytes were extracted from rat lumbar vertebrae, isolated by enzyme digestion and P2 and P4 generations selected for a 6-day in vitro culture. The specimens were photographed microscopically to observe the cellular differences by alizarin red staining. Type Ⅱ collagen marker gene, transcription factor SOX-9 gene and metabolism-related genes proteoglycan. matrix metalloproteinase (MMP)-13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5 were detected by RT-PCR to verify the degeneration model. Based on this model, the changes of growth factor TGF-β1 and calcification-related genes ankyrin (ANK), ectonucleotide pyrophosphatase (ENPP), tissue-nonspecific alkaline phosphatase (TNAP) were continuously tested. Results Compared with P2 cells, P4 cells tended to assume a spindle-shaped morphology. And alizarin red staining showed no change between them. The level of transcription factor SOX-9 of P4 cells( P4/P2 =0. 0690, P =0. 0489 ) was significantly lower than that of P2 cells. Type Ⅱ collagen ( P4/P2 = 0. 0535, P = 0. 009 ) and proteoglycan ( P4/P2 = 0. 2672, P = 0. 0343 ) were also significantly lower than those of P2 cells. No significant changes were observed in other metabolism-related genes. TGF-β1 ( P4/P2 = 0. 5934, P =0.0482) was significantly lower. The expressions of TNAP( P4/P2 = 0.0385, P =0.0139) and ANK (P4/P2 =0. 2121, P =0. 0009) were significantly lower. But ENPP showed no significant change. Conclusion P4 endplate chondrocytes undergo natural degeneration in vitro with the rising passage number. Type Ⅱ collagen, SOX-9 and proteoglycan are significantly reduced. Endogenous TGF-β1 gene and calcificationrelated genes are down-regulated. The decrease of ANK gene may be caused by the down-regulation of endogenous TGF-β1. Modulating the expression of endogenous TGF-β1 gene in endplate chondrocytes may become a new therapeutic approach for the degeneration of intervertebral disc.