中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2009年
7期
636-639
,共4页
邓艳琴%王加熊%林代华%陈亮%王灵岚
鄧豔琴%王加熊%林代華%陳亮%王靈嵐
산염금%왕가웅%림대화%진량%왕령람
布鲁氏菌%鉴定%AMOS-PCR%MLVA
佈魯氏菌%鑒定%AMOS-PCR%MLVA
포로씨균%감정%AMOS-PCR%MLVA
Brucella%Identification%AMOS-PCR%MLVA
目的 利用分子生物学方法鉴定福建省布鲁氏菌分离株.方法 对布鲁氏菌分离株进行AMOS-PCR和MLVA分型.结果 3株福建省分离株经AMOS-PCR鉴定为羊种,与传统生物学分型一致;MLVA分型将其分为3个基因型,聚类分析鉴定为羊种.结论 AMOS-PCR、MLVA分型可以作为我省布鲁氏菌分离株鉴定的辅助手段.
目的 利用分子生物學方法鑒定福建省佈魯氏菌分離株.方法 對佈魯氏菌分離株進行AMOS-PCR和MLVA分型.結果 3株福建省分離株經AMOS-PCR鑒定為羊種,與傳統生物學分型一緻;MLVA分型將其分為3箇基因型,聚類分析鑒定為羊種.結論 AMOS-PCR、MLVA分型可以作為我省佈魯氏菌分離株鑒定的輔助手段.
목적 이용분자생물학방법감정복건성포로씨균분리주.방법 대포로씨균분리주진행AMOS-PCR화MLVA분형.결과 3주복건성분리주경AMOS-PCR감정위양충,여전통생물학분형일치;MLVA분형장기분위3개기인형,취류분석감정위양충.결론 AMOS-PCR、MLVA분형가이작위아성포로씨균분리주감정적보조수단.
AMOS-PCR and MLVA were carried out to identify the Brucella strains isolated in Fujian province, which were classified as B. melitensis biovar 2 or 3 by conventional microbiological tests. All these 3 isolates were identified to be B. melitensis by AMOS-PCR. The genetic patterns obtained by MLVA were queried in the Brucella 2007 database and clustered with B. melitensis strains. It is evident that these two molecular assays may be used as the assistant tools in the identification of Brucella strains.
