CAJ | 학술논문

[目的]分析帝企鹅(Aptenodytes forsteri)CHD基因序列,以鉴定性别.[方法]采用苯酚∶氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA,运用P2/P8引物扩增CHD基因片段,将PCR产物克隆到T-Vector,利用NCBI的Blast 程序,以短趾鹰(Circaetus gallicus)同源序列为比对参照,将帝企鹅CHD 基因片段序列与GenBank 中的基因片段序列进行同源性比较分析,鉴定帝企鹅的性别.[结果]测序结果表明,CHD-Z和CHD-W基因的PCR产物大小分别为378、387 bp,雌雄结果并不明显,不易区分开来.Blast比对结果表明,帝企鹅LD、EK、ZF为雄性,帝企鹅DG、YA、YY为雌性.[结论]结合PCR扩增和测序分析CHD基因,是单态性鸟类性别鉴定的有效和准确的方法.
[목적]분석제기아(Aptenodytes forsteri)CHD기인서렬,이감정성별.[방법]채용분분∶록방추제법제취6지미지성별적제기아혈액중적기인조DNA,운용P2/P8인물확증CHD기인편단,장PCR산물극륭도T-Vector,이용NCBI적Blast 정서,이단지응(Circaetus gallicus)동원서렬위비대삼조,장제기아CHD 기인편단서렬여GenBank 중적기인편단서렬진행동원성비교분석,감정제기아적성별.[결과]측서결과표명,CHD-Z화CHD-W기인적PCR산물대소분별위378、387 bp,자웅결과병불명현,불역구분개래.Blast비대결과표명,제기아LD、EK、ZF위웅성,제기아DG、YA、YY위자성.[결론]결합PCR확증화측서분석CHD기인,시단태성조류성별감정적유효화준학적방법.
[Objective] The research aimed to analyze the CHD gene sequence of Emperor Penguin (Aptenodytes forsteri) to identify its sex. [Method] The genetic DNA was extracted from the blood of 6 A.forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex. [Result] The sequencing results showed that the PCR products of CHD-Z and CHD-W were 378、387 bp respectively, and sex character was not significant for dividing sex. The Blast comparative results showed that A.forsteri LD, EK and ZF was male, and A.forsteri DG, YA and YY was female. [Conclusion] The method of combining PCR amplification and sequencing to analyze the CHD gene is an accurate and reliable way to determine sex of monomorphic avian.