CAJ | 학술논문

利用高效液相色谱-电感耦合等离子体质谱联用技术研究了海带、羊栖菜、紫菜及浒苔等干海产品中的砷含量及其化学形态.实验表明,干海产品中主要砷形态为3种未知砷化物,对3种未知砷化物进行了表征.采用高效液相色谱与飞行时间质谱联用技术对羊栖菜和海带中的3种未知砷化物的分子量,分别为329.0599, 483.0738和409.0162,通过对比,确定这3个未知峰分别为3种砷糖类物质: DMA-glycerol ribose, DMA-phosphate ribose和DMA-sulfate ribose.对样品中砷的定量分析结果显示,干海产品中砷总量虽然超出我国国标中关于海产品中砷限量标准规定的20倍以上,但大多数植物性干海产品中主要的砷形态为毒性较低的砷糖类物质DMA-sulfate ribose(U4),其含量约占可提取砷量的51.1%～80.3%,海带中的砷主要以DMA-phosphate ribose(U3)的形式存在,占总可提取砷的48.9%.
이용고효액상색보-전감우합등리자체질보련용기술연구료해대、양서채、자채급호태등간해산품중적신함량급기화학형태.실험표명,간해산품중주요신형태위3충미지신화물,대3충미지신화물진행료표정.채용고효액상색보여비행시간질보련용기술대양서채화해대중적3충미지신화물적분자량,분별위329.0599, 483.0738화409.0162,통과대비,학정저3개미지봉분별위3충신당류물질: DMA-glycerol ribose, DMA-phosphate ribose화DMA-sulfate ribose.대양품중신적정량분석결과현시,간해산품중신총량수연초출아국국표중관우해산품중신한량표준규정적20배이상,단대다수식물성간해산품중주요적신형태위독성교저적신당류물질DMA-sulfate ribose(U4),기함량약점가제취신량적51.1%～80.3%,해대중적신주요이DMA-phosphate ribose(U3)적형식존재,점총가제취신적48.9%.
Arsenic and its species in dry seafood products(DSPs) Kelp, Sargassum fusiform, Laver, Enteromorpha prolifera, were systematically determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS). It was found that the of arsenic species in botanic DSPs are three unknown arsenicals(U_2, U_3 and U_4), which are speculated as arsenosugars and take approximate 63.5% 92.7% of all arsenic species detected. These arsenosugars were indentified as dimethylarsinic(DMA)-glycerol ribose, DMA-phosphate ribose and DMA-sulfate ribose by their accuracy molecular weight 329.0599, 483.0738 and 409.0162, using direct confirmatory of HPLC-TOF-MS. The quantitative analysis of these arsenic species shows that although the total arsenic exceeds the National Standard value, most of the marine plants DSPs are safe to eat. The main component in most marine plants is DMA-sulfate ribose(51.1%-80.3%), while the main component of Kelp is DMA-phosphate ribose(48.9%). The sum of those three arsenosugars was 63.5%-92.7% of peak areas in all arsenic species detected.