CAJ | 학술논문

目的探讨α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)在肺间质纤维化中的意义.方法发将64只Wistar大鼠随机分为对照组和博莱霉素模型组.在第3天、第7天、第14天和第28天,HE染色观察大鼠肺组织结构的改变,免疫组织化学法测定肺组织α-SMA和Ⅰ型胶原的表达,同时用流式细胞术检测肺组织单细胞悬液α-SMA的相对含量.结果博莱霉素模型组大鼠肺组织α-SMA和Ⅰ型胶原的表达在各时间段均高于对照组(P<0.05),并且两种因子表达呈正相关.同时,博莱霉素模型组各时间段两种因子测定结果均结果的差异有统计学意义(P<0.05).结论经肺间质纤维化大鼠肺组织α-SMA表达增高,预示肌纤维母细胞的形成,导致Ⅰ型胶原堆积,从而引起肺纤维化.
목적 탐토α평활기기동단백(α-smooth muscle actin,α-SMA)재폐간질섬유화중적의의.방법 발장64지Wistar대서수궤분위대조조화박래매소모형조.재제3천、제7천、제14천화제28천,HE염색관찰대서폐조직결구적개변,면역조직화학법측정폐조직α-SMA화Ⅰ형효원적표체,동시용류식세포술검측폐조직단세포현액α-SMA적상대함량.결과 박래매소모형조대서폐조직α-SMA화Ⅰ형효원적표체재각시간단균고우대조조(P<0.05),병차량충인자표체정정상관.동시,박래매소모형조각시간단량충인자측정결과 균결과 적차이유통계학의의(P<0.05).결론 경 폐간질섬유화대서폐조직α-SMA표체증고,예시기섬유모세포적형성,도치Ⅰ형효원퇴적,종이인기폐섬유화.
Objective To investigate the expression of α-smooth muscle actin (α-SMA) andⅠ-collagen(col-Ⅰ) in pathogenesis of pulmonary interstitial fibrosis. Methods Sixty-four Wistar rats weredivided into two groups, control group: instilled normal saline into trachea and bleomycin (BM) group:instilled BM into trachea. The course was sacrificed 3,7,14,28 days and at each time-point, the lung tissuemorphological changes were examined by histopathology, α-SMA and col-Ⅰ expressions were studied byimmunohistochemistry. Flow cytometry was utilized to quantitatively detect the precision of α-SMA in a cellsuspension of lung tissues. Results At all time-points, lung tissues α-SMA and col-Ⅰ increasedsignificantly in BM group than that in control group ( P < 0.05). Expression of col-Ⅰ was positivelycorrelated with that of α-SMA. There was significant differenee between expression of α-SMA and that ofcol-Ⅰ in BM group at all time-points( P<0.05). Conculusions The increase of α-SMA in pulmonary tissuesof BM-induced pulmonary fibrosis rats suggests the formation of myofibroblast, which may be involved inaccumulation of col-Ⅰ and contributed to pulmonary fibrosis.