CAJ | 학술논문

目的评价桑菊清解汤对大鼠呼吸机相关性肺损伤的影响.方法健康成年SD大鼠36只,雌雄不拘,体重300～ 350 g,采用随机数字表法,将其随机分为3组(n=12):对照组(C组)、机械通气组(V组)和桑菊清解汤组(SJ组).采用大潮气量(VT=40 ml/kg)机械通气2.5h制备大鼠呼吸机相关性肺损伤模型.SJ组于术前10 d采用桑菊清解汤300 g灌胃,1次/d,于第10次灌胃后2h时开始制备模型,V组和C组给予等容量生理盐水.于机械通气前(T0)、机械通气结束(T1)、机械通气后30 min(T2)时采集股动脉血样测定动脉血气,计算呼吸指数(RI)和氧合指数(OI),然后处死大鼠,取肺组织,采用双抗体夹心ELISA法测定肺组织TNF-α、IL-6和IL-10的含量,测定肺组织湿/干重(W/D)比,光镜下观察肺组织病理学结果.结果与C组比较,V组和SJ组T1.2时RI升高,OI降低,肺组织TNF-α、IL-6、IL-10含量和W/D比升高(P＜0.05)；与V组比较,SJ组RI降低,OI升高(P＜0.05),SJ组肺组织TNF-α、IL-6含量和W/D比降低,IL-10含量升高(P＜0.05).SJ组肺组织病理学损伤较V组减轻.结论桑菊清解汤可减轻呼吸机相关性肺损伤,其机制与抑制炎性反应有关.
목적 평개상국청해탕대대서호흡궤상관성폐손상적영향.방법 건강성년SD대서36지,자웅불구,체중300～ 350 g,채용수궤수자표법,장기수궤분위3조(n=12):대조조(C조)、궤계통기조(V조)화상국청해탕조(SJ조).채용대조기량(VT=40 ml/kg)궤계통기2.5h제비대서호흡궤상관성폐손상모형.SJ조우술전10 d채용상국청해탕300 g관위,1차/d,우제10차관위후2h시개시제비모형,V조화C조급여등용량생리염수.우궤계통기전(T0)、궤계통기결속(T1)、궤계통기후30 min(T2)시채집고동맥혈양측정동맥혈기,계산호흡지수(RI)화양합지수(OI),연후처사대서,취폐조직,채용쌍항체협심ELISA법측정폐조직TNF-α、IL-6화IL-10적함량,측정폐조직습/간중(W/D)비,광경하관찰폐조직병이학결과.결과 여C조비교,V조화SJ조T1.2시RI승고,OI강저,폐조직TNF-α、IL-6、IL-10함량화W/D비승고(P＜0.05)；여V조비교,SJ조RI강저,OI승고(P＜0.05),SJ조폐조직TNF-α、IL-6함량화W/D비강저,IL-10함량승고(P＜0.05).SJ조폐조직병이학손상교V조감경.결론 상국청해탕가감경호흡궤상관성폐손상,기궤제여억제염성반응유관.
Objective To investigate the effects of Sangju Qingjie Decoction on the ventilator-induced lung injury in rats.Methods Thirty-six healthy male SD rats of both sexes,weighing 300-350 g,were randomly divided into 3 groups(n =12 each):control group(group C); mechanical ventilation group(group V)and Sangju Qingjie Decoction group(group SJ).The animals were anesthetized with intraperitoneal 3％ urethane 1.4 g/kg and tracheostomized.The femoral artery and vein were cannulated for blood sampling and drug administration.The animals were mechanically ventilated for 2.5 h(VT =40 ml/kg,RR 40 bpm,I:E =1:1,FiO2 21％).Ingroup SJ,Sangju Qingjie Decoction 300 g was injected into the stomach through a gastric tube once a day for 10 consecutive days,and mechanical ventilation was performed 2 h after the last injection.While in groups V and C,the equal volume of normal saline was given instead of Sangju Qingjie Decoction.Arterial blood samples were collected before ventilation,at the end of ventilation,and at 30 min after ventilation(T0-2)for blood gas analysis.Respiratory index(RI)and oxygenation index(OI)were calculated.The animals were then sacrificed and the lungs were removed for microscopic examination and determination of the content of TNF-α,IL-6 and IL-10 and W/D lung weight ratio.Results Compared with group C,RI at T1,2,the content of TNF-o,IL-6 and IL-10 and W/D ratio were significantly increased,and OI was significantly decreased at T1.2 in groups V and SJ(P ＜ 0.05).Compared with group V,RI at T1,2,the content of TNF-α and IL-6 and W/D ratio were significantly decreased,and OI at T1,2 and the content of IL-10 were significantly increased in group SJ(P ＜ 0.05).The pathological damage was attenuated in group SJ compared with group V.Conclusion Sangju Qingjie Decoction can attenuate the ventilator-induced lung injury through inhibition of inflammatory response in rats.