CAJ | 학술논문

目的探讨吗啡对人胃癌MGC-803细胞磷酸酶基因(PTEN)和NF-κB活性的影响.方法胃癌MGC-803细胞随机分为对照组和吗啡组(n=6):对照组不加任何药物,吗啡组将吗啡加入细胞培养液中使吗啡终浓度为0.1μmol/L.孵育24 h后应用流式细胞仪检测细胞凋亡情况,应用RT-PCR和Western blot法检测胃癌MGC-803细胞PTEN表达和NF-κB活性.结果与对照组比较,吗啡组胃癌MGC-803细胞凋亡率升高,PTEN表达上调,NF-κB活性降低(P＜0.05).结论吗啡可通过上调PTEN表达,降低NF-κB活性促进胃癌MGC-803细胞凋亡.
목적 탐토마배대인위암MGC-803세포린산매기인(PTEN)화NF-κB활성적영향.방법 위암MGC-803세포수궤분위대조조화마배조(n=6):대조조불가임하약물,마배조장마배가입세포배양액중사마배종농도위0.1μmol/L.부육24 h후응용류식세포의검측세포조망정황,응용RT-PCR화Western blot법검측위암MGC-803세포PTEN표체화NF-κB활성.결과 여대조조비교,마배조위암MGC-803세포조망솔승고,PTEN표체상조,NF-κB활성강저(P＜0.05).결론 마배가통과상조PTEN표체,강저NF-κB활성촉진위암MGC-803세포조망.
Objective To investigate the effects of morphine on PTEN expression and NF-κB activity in human gastric carcinoma cell line MGC-803.Methods The human gastric cancer cell line MGC-803 was purchased from Cell Biology Research Institute,Chinese Academy of Sciences,and cultured in DMEM liquid culture medium.The cells were randomly divided into 2 groups(n = 6 each): control group and morphine group.The cells was exposed to 0.1 μmol/L morphine in morphine group.The apoptosis was assessed by flow cytometry after being incubated with morphine for 24 h.PTEN expression and NF-κB activity were detected using RT-PCR and Western blot.Results The apoptotic rate was significantly increased,PTEN expression was up-regulated and NF-κB activity was significantly decreased in morphine group compared with control group(P ＜ 0.05).Conclusion Morphine can promote the apoptosis in human gastric cancer cells by up-regulating PTEN expression and decreasing NF-κB activity.