中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2010年
2期
89-92
,共4页
原津津%潘晨%黎环%许利军
原津津%潘晨%黎環%許利軍
원진진%반신%려배%허리군
肿瘤坏死因子α%多态性%肝炎病毒,乙型%突变
腫瘤壞死因子α%多態性%肝炎病毒,乙型%突變
종류배사인자α%다태성%간염병독,을형%돌변
Tumor necrosis factor-alpha%Polymorphism%Hepatitis B virus%Mutation
目的 研究慢性乙型肝炎病毒(HBV)感染者肿瘤坏死因子α(TNFα)-308位点的基因多态性与HBV C基因区突变的关系.方法 对95例慢性HBV感染者进行研究,采用聚合酶链反应.限制性片断长度多态性(PCR-RFLP)技术分析患者TNFα-308位点的多态性.对PCR产物直接测序,检测HBV C基因区nt1762/1764、nt1896、nt1899、nt1862、aa60、as87及aa97位点是否存在突变.采用Fisher's精确概率法比较TNFα-308位点不同基因型患者HBV C基因区常见突变的检出率.结果 TNFα-308位点共发现3种多态性,分别为G/G型63例(63/95,66.3%),G/A型28例(28/95,29.5%),A/A型4例(4/95,4.2%).TNFα-308位点G/G、G/A、A/A基因型患者HBV C基因区aa87位点及as97位点突变型检出率均分别为39.3%(24/61)、11.5%(3/26)和50.0%(2/4),差异有统计学意义(F=7.658,P<0.05);而nt1762/1764、nt1896、nt1899、nt1862以及aa60位点突变型检出率比较差异无统计学意义(F值分别为0.669、1.542、1.123、2.420和0.966,P值均>0.05).结论 慢性HBV感染者TNFα-308位点G/A基因型相对于G/G基因型不易发生HBV抗原性变异,有利于HBV的清除.
目的 研究慢性乙型肝炎病毒(HBV)感染者腫瘤壞死因子α(TNFα)-308位點的基因多態性與HBV C基因區突變的關繫.方法 對95例慢性HBV感染者進行研究,採用聚閤酶鏈反應.限製性片斷長度多態性(PCR-RFLP)技術分析患者TNFα-308位點的多態性.對PCR產物直接測序,檢測HBV C基因區nt1762/1764、nt1896、nt1899、nt1862、aa60、as87及aa97位點是否存在突變.採用Fisher's精確概率法比較TNFα-308位點不同基因型患者HBV C基因區常見突變的檢齣率.結果 TNFα-308位點共髮現3種多態性,分彆為G/G型63例(63/95,66.3%),G/A型28例(28/95,29.5%),A/A型4例(4/95,4.2%).TNFα-308位點G/G、G/A、A/A基因型患者HBV C基因區aa87位點及as97位點突變型檢齣率均分彆為39.3%(24/61)、11.5%(3/26)和50.0%(2/4),差異有統計學意義(F=7.658,P<0.05);而nt1762/1764、nt1896、nt1899、nt1862以及aa60位點突變型檢齣率比較差異無統計學意義(F值分彆為0.669、1.542、1.123、2.420和0.966,P值均>0.05).結論 慢性HBV感染者TNFα-308位點G/A基因型相對于G/G基因型不易髮生HBV抗原性變異,有利于HBV的清除.
목적 연구만성을형간염병독(HBV)감염자종류배사인자α(TNFα)-308위점적기인다태성여HBV C기인구돌변적관계.방법 대95례만성HBV감염자진행연구,채용취합매련반응.한제성편단장도다태성(PCR-RFLP)기술분석환자TNFα-308위점적다태성.대PCR산물직접측서,검측HBV C기인구nt1762/1764、nt1896、nt1899、nt1862、aa60、as87급aa97위점시부존재돌변.채용Fisher's정학개솔법비교TNFα-308위점불동기인형환자HBV C기인구상견돌변적검출솔.결과 TNFα-308위점공발현3충다태성,분별위G/G형63례(63/95,66.3%),G/A형28례(28/95,29.5%),A/A형4례(4/95,4.2%).TNFα-308위점G/G、G/A、A/A기인형환자HBV C기인구aa87위점급as97위점돌변형검출솔균분별위39.3%(24/61)、11.5%(3/26)화50.0%(2/4),차이유통계학의의(F=7.658,P<0.05);이nt1762/1764、nt1896、nt1899、nt1862이급aa60위점돌변형검출솔비교차이무통계학의의(F치분별위0.669、1.542、1.123、2.420화0.966,P치균>0.05).결론 만성HBV감염자TNFα-308위점G/A기인형상대우G/G기인형불역발생HBV항원성변이,유리우HBV적청제.
Objective To investigate the association between polymorphism of TNFα-308 and mutations of HBV C region in patients with chronic HBV infection. Methods Ninety-five patients with chronic HBV infection were recruited in the study. The single nucleotide polymorphism(SNP) of TNFα-308 Was determined by restriction fragment length polymorphism(RFLP). Mutations of nt1762/1764, nt1896, nt1899, nt1862, as60, aa87 and as97 in HBV C region were detected by direct sequencing after PCR amplification. Mutations of the above points among different genotypes were compared by Fisher's exget test. Results Three different genotypes G/G(63/95, 66.3%), G/A(28/95, 29.5%) and A/A(4/95, 4.2%) were found in TNFα-308 site. The rates of mutations of aa87 and aa97 points in patients with G/G, G/A and A/A genotype were 39.3%(24/61), 11.5%(3/26) and 50.0% (2/4), respectively(F=7.658, P<0.05);while the mutation rates of nt1762/1764, nt1896, nt1864, nt1899 and aa60 were of no statistical significance among different genotypes(F=0.669, 1.542, 1.123, 2.420 and 0.966, P>0.05). Conclusion Compared with G/G genotype, antigenicity of HBV may be more stable in patients with TNFα-308 G/A genotype, which is beneficial to HBV clearance.
