中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
7期
496-501
,共6页
刘薇薇%陈娟%刘移民%李素梅%余卫%陈嘉榆%张程%唐丽娟%林琛莅%钟雪云
劉薇薇%陳娟%劉移民%李素梅%餘衛%陳嘉榆%張程%唐麗娟%林琛蒞%鐘雪雲
류미미%진연%류이민%리소매%여위%진가유%장정%당려연%림침리%종설운
石英%肺纤维化%模型,动物
石英%肺纖維化%模型,動物
석영%폐섬유화%모형,동물
Quartz%Pulmonary fibrosis%Models,animal
目的 比较石英尘和博莱霉素诱导的大鼠肺纤维化模型中的肺泡炎和早期纤维化改变,并对其机制进行探讨.方法 将大鼠随机分为SiO2组(14只,气管内注入40mg/ml SiO2混悬液1 ml)、BLM组(14只,气管内注入5 mg/kg BLM A5)和对照组(14只,气管内注入1 ml无菌生理盐水).在造模后7、14 d每组各处死7只动物,取肺组织病理切片行HE染色,对肺泡炎进行计分,行饱和苦味酸天狼猩红染色,采用图像分析系统测得各组胶原面积后,进行定量分析,免疫组织化学技术检测肺组织中CD68和TNF-α蛋白表达情况,采用图像分析计算累积吸光度值,进行半定量分析.结果 (1)HE染色光学显微镜下可见,BLM组7d时肺泡炎(肺泡炎评分2.814±0.832)最明显,偏振光下显示BLM组14d时肺纤维化[胶原面积(1284.57±554.72)μm2)]最严重,均明显高于对照组和同期SiO2组,差异有统计学意义(P＜0,05).(2)免疫组化结果显示,BLM组7 d时TNF-α表达最高(17.100±1.831),明显高于对照组(0.420±0.020)、SiO2组7 d(7.909±1.275)及BLM组14d(13.506±1.454),差异均有统计学意义(P＜0.05);在SiO2组14 d时TNF-α表达为22.778±2.512,明显高于BLM组(14 d)、对照组及SiO2组(7 d),差异有统计学意义(P＜0.05).14 d时,SiO2组CD68表达明显高于对照组、BLM组(14 d)及SiO2组(7d),差异有统计学意义(P＜0.05).结论 BLM诱导的大鼠肺损伤模型的早期肺泡炎重于SiO2诱导的大鼠肺损伤模型,纤维化进程早于SiO2诱导的大鼠肺损伤模型,TNF-α在两种模型的病程中均起着重要的作用,而巨噬细胞更为持续地参与了SiO2诱导的肺纤维化.
目的 比較石英塵和博萊黴素誘導的大鼠肺纖維化模型中的肺泡炎和早期纖維化改變,併對其機製進行探討.方法 將大鼠隨機分為SiO2組(14隻,氣管內註入40mg/ml SiO2混懸液1 ml)、BLM組(14隻,氣管內註入5 mg/kg BLM A5)和對照組(14隻,氣管內註入1 ml無菌生理鹽水).在造模後7、14 d每組各處死7隻動物,取肺組織病理切片行HE染色,對肺泡炎進行計分,行飽和苦味痠天狼猩紅染色,採用圖像分析繫統測得各組膠原麵積後,進行定量分析,免疫組織化學技術檢測肺組織中CD68和TNF-α蛋白錶達情況,採用圖像分析計算纍積吸光度值,進行半定量分析.結果 (1)HE染色光學顯微鏡下可見,BLM組7d時肺泡炎(肺泡炎評分2.814±0.832)最明顯,偏振光下顯示BLM組14d時肺纖維化[膠原麵積(1284.57±554.72)μm2)]最嚴重,均明顯高于對照組和同期SiO2組,差異有統計學意義(P＜0,05).(2)免疫組化結果顯示,BLM組7 d時TNF-α錶達最高(17.100±1.831),明顯高于對照組(0.420±0.020)、SiO2組7 d(7.909±1.275)及BLM組14d(13.506±1.454),差異均有統計學意義(P＜0.05);在SiO2組14 d時TNF-α錶達為22.778±2.512,明顯高于BLM組(14 d)、對照組及SiO2組(7 d),差異有統計學意義(P＜0.05).14 d時,SiO2組CD68錶達明顯高于對照組、BLM組(14 d)及SiO2組(7d),差異有統計學意義(P＜0.05).結論 BLM誘導的大鼠肺損傷模型的早期肺泡炎重于SiO2誘導的大鼠肺損傷模型,纖維化進程早于SiO2誘導的大鼠肺損傷模型,TNF-α在兩種模型的病程中均起著重要的作用,而巨噬細胞更為持續地參與瞭SiO2誘導的肺纖維化.
목적 비교석영진화박래매소유도적대서폐섬유화모형중적폐포염화조기섬유화개변,병대기궤제진행탐토.방법 장대서수궤분위SiO2조(14지,기관내주입40mg/ml SiO2혼현액1 ml)、BLM조(14지,기관내주입5 mg/kg BLM A5)화대조조(14지,기관내주입1 ml무균생리염수).재조모후7、14 d매조각처사7지동물,취폐조직병리절편행HE염색,대폐포염진행계분,행포화고미산천랑성홍염색,채용도상분석계통측득각조효원면적후,진행정량분석,면역조직화학기술검측폐조직중CD68화TNF-α단백표체정황,채용도상분석계산루적흡광도치,진행반정량분석.결과 (1)HE염색광학현미경하가견,BLM조7d시폐포염(폐포염평분2.814±0.832)최명현,편진광하현시BLM조14d시폐섬유화[효원면적(1284.57±554.72)μm2)]최엄중,균명현고우대조조화동기SiO2조,차이유통계학의의(P＜0,05).(2)면역조화결과현시,BLM조7 d시TNF-α표체최고(17.100±1.831),명현고우대조조(0.420±0.020)、SiO2조7 d(7.909±1.275)급BLM조14d(13.506±1.454),차이균유통계학의의(P＜0.05);재SiO2조14 d시TNF-α표체위22.778±2.512,명현고우BLM조(14 d)、대조조급SiO2조(7 d),차이유통계학의의(P＜0.05).14 d시,SiO2조CD68표체명현고우대조조、BLM조(14 d)급SiO2조(7d),차이유통계학의의(P＜0.05).결론 BLM유도적대서폐손상모형적조기폐포염중우SiO2유도적대서폐손상모형,섬유화진정조우SiO2유도적대서폐손상모형,TNF-α재량충모형적병정중균기착중요적작용,이거서세포경위지속지삼여료SiO2유도적폐섬유화.
Objective To compare the pulmonary alveolitis and the early fibrosis of pulmonary fibrosis induced by quartz dust and bleomycin in rats, and investigate their mechanism. Methods The female rats were divided into three groups: control group exposed to normal saline by the trachea; SiO2 group exposed to SiO2 by the trachea; BLM group exposed to BLM A5 by the trachea. Each half of the animals were sacrificed on the 7th andl4th day after expoasure. The lungs of rats were collected to observe pulmonary alveolitis by HE staining and to observe fibrosis by saturated picric acid sirius red staining. The expression of tumor necrosis factor-α (TNF-α) and CD68 in pulmonary tissues were analyzed quantitatively by immunohistochmistry and image analysis system. Results (1) The alveolitis and pulmonary fibrosis of rats in both SiO2 group and BLM group were became more serious gradually over time, HE staining under light microscope showed that BLM group on the 7th day had the most obvious alveolitis (2.814±0.832), the saturated picric acid sirius red staining under polarized light showed that BLM group on the 14th day had the worst pulmonary fibrosis (1284.57±554.72), which were significantly higher than those (103.69±18.29 and 111.78±37.45) in control group and SiO2 group on the 7th day (P＜0.05). (2) The results of immunohistochmistry examination indicated that the expression (17.100±1.831) of TNF-α in the BLM group on the 7th day was significantly higher than those (0.451 ±0.441, 7.909±1.275 and 13.506±1.454) in control group, SiO2 group on 7th day and BLM group on 14th day (P＜0.05). The expression (22.778 ±2.512) of TNF-a in the SiO2 group on the 14th day was significantly higher than those in control group, SiO2 group on 7th day and BLM group on 14th day (P＜0.05).The expression (134.941 ±35.951) of CD68 in the SiO2 group on the 14th day was significantly higher than those in control group,SiO2 group on 7th day and BLM group on 14th day(P＜0.05).Conclusion The early alveolitis of BLM-induced lung injury model was more serious than that of SiO2-induced lung injury model,and the fibrosis process of BLM-induced lung injury model was earlier than that of SiO2-induced lung injury model.TNF-α plays an important role in the conrse of both models.but macrophages is involved in Si06nduced pulmonary in a more continuous way than in BLM-induced pulmonary fibrosis.
