CAJ | 학술논문

目的观察调节性T细胞在原发性干燥综合征(pSS)患者外周血和唇腺中的表达.方法选取23例pSS患者和15名健康对照组,通过流式细胞术检测2组之间外周血调节性T细胞的表达情况,采用免疫磁珠分选外周血CD4+T细胞,提取叉头样转录因子(Foxp3)的mRNA,进行实时荧光定量的聚合酶链反应(PCR)检测.选取23例pSS患者和5例除外pSS的原发性胆汁性肝硬化(PBC)患者,通过免疫组织化学方法分析2组之间唇腺组织中CD4+和Foxp3+T细胞的表达差异.采用t检验进行统计学处理.结果 pSS组[(5±6)％]外周血Foxp3+T细胞的表达比健康对照组[(10±5)％]明显降低(t=2.190; P=0.036); Foxp3-mRNA的含量pSS组(0.08±0.05)与健康对照组(0.09±0.03)之间差异无统计学意义(t=0.695;P＞0.05)；与PBC对照组唇腺中CD4+T细胞[(11±6)％]和Foxp3+T细胞[(3.2±1.1)％]比较,pSS患者唇腺中CD4+T细胞[(30±10)％]和Foxp3+T细胞[(10.7±5.8)％]表达增加(t=4.072,2.840;P均＜0.05).结论调节性T细胞在pSS外周血表达降低,而在唇腺中的表达并未减少,二者之间表达不一致,Foxp3参与了pSS的发病.
목적 관찰조절성T세포재원발성간조종합정(pSS)환자외주혈화진선중적표체.방법 선취23례pSS환자화15명건강대조조,통과류식세포술검측2조지간외주혈조절성T세포적표체정황,채용면역자주분선외주혈CD4+T세포,제취차두양전록인자(Foxp3)적mRNA,진행실시형광정량적취합매련반응(PCR)검측.선취23례pSS환자화5례제외pSS적원발성담즙성간경화(PBC)환자,통과면역조직화학방법분석2조지간진선조직중CD4+화Foxp3+T세포적표체차이.채용t검험진행통계 학처리.결과 pSS조[(5±6)％]외주혈Foxp3+T세포적표체비건강대조조[(10±5)％]명현강저(t=2.190; P=0.036); Foxp3-mRNA적함량pSS조(0.08±0.05)여건강대조조(0.09±0.03)지간차이무통계학의의(t=0.695;P＞0.05)；여PBC대조조진선중CD4+T세포[(11±6)％]화Foxp3+T세포[(3.2±1.1)％]비교,pSS환자진선중CD4+T세포[(30±10)％]화Foxp3+T세포[(10.7±5.8)％]표체증가(t=4.072,2.840;P균＜0.05).결론 조절성T세포재pSS외주혈표체강저,이재진선중적표체병미감소,이자지간표체불일치,Foxp3삼여료pSS적발병.
Objective This study was designed to investigate the expression of T regulatory cells (Treg) in the peripheral blood and salivary gland of patients with primary Sj(o)gren's syndrome (pSS).Methods The expression of Treg was measured by flow-cytometry analysis in 23 pSS patients and 15 healthy controls.CD4 positive cells were sorted by immunomagnetic beads,Forkhead box protein P3 (Foxp3) mRNA was extracted,real time polymerase chain reaction (RT-PCR) was used to measure Foxp3 mRNA.For the study of salivary gland,five primary billary cirrhosis (PBC) patients without pSS were selected as the control group,the expression of CD4+T cells and Foxp3+ T cells in salivary gland was examined by immunohistochemistry.The result was analyzed by t test.Results The expression of Treg in the peripheral blood of pSS patients [(5±6)％] was lower than healthy controls [(10±5)％] (t=2.190,P=0.036).There was no significant difference in Foxp3-mRNA expression between pSS patients (0.08±0.05) and controls(0.09±0.03 ) (t=0.695,P＞0.05 ).The expression of CD4+ T cells [ ( 30± 10 )％ ] and Foxp3+ T cells [ ( 10.7±5.8 ) ％ ] in the salivary gland of pSS patients increased significantly when compared with PBC control group [CD4+T (11±6)％ and Foxp3+T(3.2+1.1)％ ] (t=4.072,2.840; P＜0.05).Conclusion For pSS patients,the expression of Treg decreases in the peripheral blood,but Treg increases in the salivary gland.The results of this study suggest that Foxp3plays an important role in the pathogenesis of pSS.