CAJ | 학술논문

目的研究14-3-3ε在食管鳞癌及正常食管黏膜中的表达及其临床意义.方法免疫组化检测72例食管鳞癌及其配对癌旁正常食管黏膜的14-3-3ε表达水平.运用X2检验分析肿瘤组织14-3-3ε表达与临床病理因素的关系.运用Kaplan-Meier法分析肿瘤组织14-3-3ε与患者术后总生存时间的关系.结果 14-3-3ε在食管鳞癌中阳性率和强阳性率分别为77.8%和27.8%;在正常食管黏膜中表达阳性率和强阳性率分别为43.1%和2.8%,其肿瘤高表达差异有统计学意义(P＜0.01).在56例14-3-3ε阳性的肿瘤中,37例(66.1%)为细胞质表达,4(7.1%)例为核表达,15例(26.8%)为质核共表达;在31例14-3-3ε阳性的正常食管黏膜中,无细胞质表达,13例(41.9%)为细胞核表达,18例(58.1%)为质核共表达.该蛋白在肿瘤细胞与正常细胞的定位差异有统计学意义(P＜0.01).通过与临床病理因素的进一步分析,发现肿瘤细胞14-3-3ε表达强阳性与淋巴结转移(P=0.028)和术后5年生存率相关(P=0.018).生存曲线提示肿瘤14-3-3ε强阳性患者预后不良(Log-rank,P=0.031).14-3-3e定位差异与各临床病理因素及术后生存无显著相关性.结论 14-3-3ε在食管癌中表达上调;该蛋白在肿瘤中多表达于细胞质,在正常食管黏膜中多表达于细胞核;14-3-3ε表达水平与淋巴结转移和预后不良呈正相关.
목적 연구14-3-3ε재식관린암급정상식관점막중적표체급기림상의의.방법 면역조화검측72례식관린암급기배대암방정상식관점막적14-3-3ε표체수평.운용X2검험분석종류조직14-3-3ε표체여림상병리인소적관계.운용Kaplan-Meier법분석종류조직14-3-3ε여환자술후총생존시간적관계.결과 14-3-3ε재식관린암중양성솔화강양성솔분별위77.8%화27.8%;재정상식관점막중표체양성솔화강양성솔분별위43.1%화2.8%,기종류고표체차이유통계학의의(P＜0.01).재56례14-3-3ε양성적종류중,37례(66.1%)위세포질표체,4(7.1%)례위핵표체,15례(26.8%)위질핵공표체;재31례14-3-3ε양성적정상식관점막중,무세포질표체,13례(41.9%)위세포핵표체,18례(58.1%)위질핵공표체.해단백재종류세포여정상세포적정위차이유통계학의의(P＜0.01).통과여림상병리인소적진일보분석,발현종류세포14-3-3ε표체강양성여림파결전이(P=0.028)화술후5년생존솔상관(P=0.018).생존곡선제시종류14-3-3ε강양성환자예후불량(Log-rank,P=0.031).14-3-3e정위차이여각림상병리인소급술후생존무현저상관성.결론 14-3-3ε재식관암중표체상조;해단백재종류중다표체우세포질,재정상식관점막중다표체우세포핵;14-3-3ε표체수평여림파결전이화예후불량정정상관.
.Objective To investigate the expression of 14-3-3ε in esophageal squamous cell carcinoma (ESCC) and relationship of 14-3-3ε and clinicopathological factors of ECSS patients. Methods The 14-3-3ε expression in tumors and adjacent normal epithelia from 72 ESCC patients was detected by immunohistochemical staining. Correlations of 14-3-3ε expression or their subcellular localization and clinicopathological factors were analyzed using Chi-squared test. Survival curves were generated according to the Kaplan-Meier method, and the statistical analysis was performed by Log-rank test. Results The expression of 14-3-3ε protein was significantly up-regulated in tumors with 77. 8% positive and 27. 8%strong positive staining, compared with paired adjacent normal epithelia with 43. 1% positive and only 2. 8%strong positive staining (P ＜ 0. 01 ). In a total of 56 positive staining tumors, 14-3-3ε was detected in cytoplasm of 37 (66. 1% ), in nucleus of 4 (7. 1% ) ,in both cytoplasm and nucleus of 15 (26. 8% ) cases.Whereas, in a total of 31 positive staining normal epithelia, 14-3-3ε was detected in cytoplasm of 0, in nucleus of 13 (41.9%), in both cytoplasm and nucleus of 18 ( 58. 1% ) cases ( P ＜ 0. 01 ). Statistical analysis revealed that strong 14-3-3ε expression was correlated with lymph node metastasis (P = 0. 028) and lower 5-year survival (P = 0. 018). The survival curves calculated by Kaplan-Meier method further showed that the patients with strong 14-3-3ε expression had a shorter survival than patients with negative or weak 14-3-3ε expression (Log-rank,P =0. 031 ). No correlation was found between subcellular localization of 14-3-3ε in tumor cells and clinicopathologic factors and prognosis of ESCC patients. Conclusion The 14-3-3εexpression is significantly up-regulated in ESCCs. It was usually located in cytoplasm of tumor cells, but nucleus of normal epithelia. Strong expression of 14-3-3ε in tumors is associated with lymph node metastasis and considered as an poor prognostic factor for ESCC.