CAJ | 학술논문

目的利用能够理想模拟脊髓受压的动物模型,分析脊髓在受压过程中的病理特点及神经电生理变化,研究脊髓损伤与神经功能异常的相关性.方法选用新西兰大耳白兔32只,随机分为对照组、轻度压迫组、中度压迫组和重度压迫组,每组8只.轻度压迫组、中度压迫组和重度压迫组为实验组,分别选用直径为1.0 mm、2.0 mm和3.0 mm的球囊导入至C6～7平面,造成轻、中、重三种程度的脊髓压迫性损伤.记录不同时间的皮层体感诱发电位(CSEP)和运动诱发电位(MEP)波形,观察动物后肢运动功能;切取损伤节段的脊髓标本行组织病理学观察.结果脊髓损伤的病理组织学改变、后肢的运动功能与诱发电位具有明显的相关性,神经元细胞损伤数目越多,白质纤维脱髓鞘越重,则CSEP的潜伏期延长和波幅降低越明显.动物脊髓压迫后,即刻进行诱发电位测试,中度压迫组CSEP波幅从4.2μV下降至2.2μV,而MEP波幅则从24.7μV下降至5.3μV,提示在监测脊髓运动功能时,MEP比CSEP更加敏感.结论选用带球囊的导管作为实验性动物颈脊髓急性压迫模型的压迫物,可使脊髓压迫实验模型简单化、标准化.在不完全脊髓损伤中,诱发电位的变化和病理损伤有明显的相关性.
목적 이용능구이상모의척수수압적동물모형,분석척수재수압과정중적병리특점급신경전생리변화,연구척수손상여신경공능이상적상관성.방법 선용신서란대이백토32지,수궤분위대조조、경도압박조、중도압박조화중도압박조,매조8지.경도압박조、중도압박조화중도압박조위실험조,분별선용직경위1.0 mm、2.0 mm화3.0 mm적구낭도입지C6～7평면,조성경、중、중삼충정도적척수압박성손상.기록불동시간적피층체감유발전위(CSEP)화운동유발전위(MEP)파형,관찰동물후지운동공능;절취손상절단적척수표본행조직병이학관찰.결과 척수손상적병리조직학개변、후지적운동공능여유발전위구유명현적상관성,신경원세포손상수목월다,백질섬유탈수초월중,칙CSEP적잠복기연장화파폭강저월명현.동물척수압박후,즉각진행유발전위측시,중도압박조CSEP파폭종4.2μV하강지2.2μV,이MEP파폭칙종24.7μV하강지5.3μV,제시재감측척수운동공능시,MEP비CSEP경가민감.결론 선용대구낭적도관작위실험성동물경척수급성압박모형적압박물,가사척수압박실험모형간단화、표준화.재불완전척수손상중,유발전위적변화화병리손상유명현적상관성.
Objective To study the characteristics of its pathologic and neurophysiologic changes of a ani-mal model of cervical spinal cord compression, and to explore the relationship between the severity of spinal cord inju-ry and its functions. Methods Thirty-two rabbits were divided into 4 groups randomly. A catheter was inserted intothe vertebral canal and a balloon was inflated to compress spinal cord in epidural space. According to the diameter ofballoon, the animals were divided into 4 groups (A, B, C, D group), Each group consisted of 8 rabbits. The corti-cal somatosensory evoked potential (CSEP) and motor evoked potential (MEP) were used to assess neurologicalfunction. Motor function behavior was scored before and on 1, 3, 7 and 14 days after surgery. Histological observa-tions were performed, pathological changes were observed by light and electron microscope. Results Spinal cordcompression resulted in a gradual increase of the peak latency and significant decrease of the peak amplitude. The la-tency and amplitude of MEP were changed more dramatically than those of CSEP. The result indicated that MEP wasmore sensitive than CSEP to the compression. Analysis also revealed that the severer the pathologic changes, the lon-ger the latency and the lower the amplitude of the evoked potentials. Conclusion The animal model presented inthis paper was simple and standardized. Evoked potentials as a noninvasive technique have great value in monitoringspinal cord function. The variance of amplitude and the latency in significantly correlate with the degree of compres-sion of the spinal cord.