CAJ | 학술논문

目的探讨毛囊无色素性黑素细胞(amelanotic melanocytes,AMMC)和干细胞因子(stem cell factor,SCF)及其受体c-kit(SCF/c-kit)信号通路在白癜风发病机制中的作用.方法采用苏木精-伊红(HE)染色和免疫组织化学方法对31例白癜风皮损头皮组织毛囊和30例正常人头皮组织毛囊进行HMB-45、NKI/beteb、c-kit 3种特异性抗体染色,观察毛囊AMMC的形态分布特征及c-kit的表达,并以HPIAS-1000彩色病理图文分析系统半定量分析3种特异性标志物表达的强弱,观察两组之间有无差异.结果白癜风皮损头皮组毛囊AMMC的NKI/beteb染色的平均阳性细胞面积率和平均光密度值与正常头皮组织相比,差异无统计学意义(P>0.05);白癜风皮损头皮毛囊组AMMC的c-kit染色平均阳性面积率和平均光密度值明显低于正常头皮组,差异具有统计学意义(P<0.05).结论白癜风头部皮损毛囊AMMC c-kit的表达异常,其可能参与了白癜风的发病过程.
목적 탐토모낭무색소성흑소세포(amelanotic melanocytes,AMMC)화간세포인자(stem cell factor,SCF)급기수체c-kit(SCF/c-kit)신호통로재백전풍발병궤제중적작용.방법 채용소목정-이홍(HE)염색화면역조직화학방법대31례백전풍피손두피조직모낭화30례정상인두피조직모낭진행HMB-45、NKI/beteb、c-kit 3충특이성항체염색,관찰모낭AMMC적형태분포특정급c-kit적표체,병이HPIAS-1000채색병리도문분석계통반정량분석3충특이성표지물표체적강약,관찰량조지간유무차이.결과 백전풍피손두피조모낭AMMC적NKI/beteb염색적평균양성세포면적솔화평균광밀도치여정상두피조직상비,차이무통계학의의(P>0.05);백전풍피손두피모낭조AMMC적c-kit염색평균양성면적솔화평균광밀도치명현저우정상두피조,차이구유통계학의의(P<0.05).결론 백전풍두부피손모낭AMMC c-kit적표체이상,기가능삼여료백전풍적발병과정.
Objective To analyse preliminarily the role of amelanotic melanocutes (AMMC) and SCF/c-kit signal pathway in the pathogenesis of vitiligo. Methods Three antibodies such as HMB-45, NKl/beteb and c-kit were used to stain sections from scalps from vitiligo patients and the healthy controls. Results There were no HMB-45 positive cells in outer root sheath(ORS) of follicle. NKI/beteb positive cells were small and located in groups at the middle and lower of outer root sheath with their retraction of the dendrites. They only expressed premelanosomal antigens but not melanosomal antigen such as HMB-45. There were no significant difference of AMMC in quantities between vitiligo patients and normal controls (P>0. 05). The expression of c-kit receptors on AMMC in follicle of depigment-ed scalps from vitiligo patients was lower than that in normal contols (P<0. 05). Conclusion Abnormal c-kit expression in AMMC in the follicle of depigmented scalps may play an important role in the pathogenesis of vitiligo.