CAJ | 학술논문

滚环DNA扩增(Rolling circle DNA amplification, RCA)是一种等温信号扩增方法,其线性扩增倍数为105,指数化扩增能力大于109,产生的扩增产物连接在固相支持物(如玻片、微孔板等)表面的DNA引物或抗体上(适宜作生物芯片研究).RCA是一种适合在芯片上(on-chip)进行信号扩增的新技术,它既能提供研究分析的敏感性和特异性,又能保持立体分析的多元性.RCA亦是一种痕量的分子检测方法,可用于极其微量的生物大分子和生物标志的检测与研究.
곤배DNA확증(Rolling circle DNA amplification, RCA)시일충등온신호확증방법,기선성확증배수위105,지수화확증능력대우109,산생적확증산물련접재고상지지물(여파편、미공판등)표면적DNA인물혹항체상(괄의작생물심편연구).RCA시일충괄합재심편상(on-chip)진행신호확증적신기술,타기능제공연구분석적민감성화특이성,우능보지입체분석적다원성.RCA역시일충흔량적분자검측방법,가용우겁기미량적생물대분자화생물표지적검측여연구.
Rolling circle DNA amplification (RCA) is an isothermal signal amplification method. The phi 29 DNA polymerase is able to synthesize DNA chains more than 70 kilobase pairs in length and achieve a 10\+5-fold linear and a 10\+9-fold or more exponential amplification. The product produced by RAC can be linked to the DNA primers or antibodies which immobilized on supporter (such as glass, microplate etc.) that make the RCA especially suitable for the researches about biochip. As a new technique RCA is well suited to on-chip signal amplification that not only provide the sensibitity and specificity of analysis but also keep the multiplexing of stereo-analysis. RCA is also a trace molecular detection method and will be exploited in research and detection of extremely trace biomacromolecule or biomarkers.