CAJ | 학술논문

在室内采用稻茎浸渍法进行了灰飞虱对氟虫腈抗性风险评估、抗性遗传分析和杀虫剂的敏感性研究.于2005年采自无锡麦田的灰飞虱种群在室内饲养43代期间用氟虫腈筛选了41代,结果此灰飞虱种群的抗性从8.4倍上升到230.5倍.根据Tabashnik介绍的方法计算,现实遗传力(h~2)分别为0.0388(1～31代)、0.2636(32～43代)和0.1113(1～43代), 表明灰飞虱对氟虫腈具有一定的抗性风险.采用稻茎浸渍法测定了2007-2008年江苏无锡和浙江长兴两地灰飞虱种群对15种杀虫剂的敏感性, 结果表明,苯基吡唑类杀虫剂氟虫腈、丁烯氟虫腈和乙虫腈的毒力最高(LC_(50)=0.2～1.7 mg/L),其次为烯啶虫胺、噻虫嗪、毒死蜱、吡蚜酮(LC_(50)=1.7～9.7 mg/L),其余杀虫剂的毒力较低；按照年度间敏感性变化,发现2008年无锡灰飞虱种群对氟虫腈和乙虫腈的敏感性比2007年降低了1.1倍.近年来氟虫腈已广泛用于防治这种害虫,但乙虫腈几乎没有使用.因此,对氟虫腈敏感性降低的大田灰飞虱种群似乎对乙虫腈存在交互抗性.通过抗(R)、感(S)亲本、正反交(F_1、 F_1')、自交(F_2)及回交(BC)后代对氟虫腈的剂量反应研究了灰飞虱对氟虫腈的抗性遗传特性,结果表明,其抗性为常染色体的不完全显性遗传[D(F_1)=0.20, D(F_1')=0.38］,抗性由2个或2个以上等位基因控制.还对灰飞虱的抗性治理进行了讨论.
재실내채용도경침지법진행료회비슬대불충정항성풍험평고、항성유전분석화살충제적민감성연구.우2005년채자무석맥전적회비슬충군재실내사양43대기간용불충정사선료41대,결과차회비슬충군적항성종8.4배상승도230.5배.근거Tabashnik개소적방법계산,현실유전력(h~2)분별위0.0388(1～31대)、0.2636(32～43대)화0.1113(1～43대), 표명회비슬대불충정구유일정적항성풍험.채용도경침지법측정료2007-2008년강소무석화절강장흥량지회비슬충군대15충살충제적민감성, 결과표명,분기필서류살충제불충정、정희불충정화을충정적독력최고(LC_(50)=0.2～1.7 mg/L),기차위희정충알、새충진、독사비、필아동(LC_(50)=1.7～9.7 mg/L),기여살충제적독력교저；안조년도간민감성변화,발현2008년무석회비슬충군대불충정화을충정적민감성비2007년강저료1.1배.근년래불충정이엄범용우방치저충해충,단을충정궤호몰유사용.인차,대불충정민감성강저적대전회비슬충군사호대을충정존재교호항성.통과항(R)、감(S)친본、정반교(F_1、 F_1')、자교(F_2)급회교(BC)후대대불충정적제량반응연구료회비슬대불충정적항성유전특성,결과표명,기항성위상염색체적불완전현성유전[D(F_1)=0.20, D(F_1')=0.38］,항성유2개혹2개이상등위기인공제.환대회비슬적항성치리진행료토론.
Risk assessment and genetic analysis on fipronil resistance and insecticide susceptibility in the small brown planthopper were conducted in laboratory by rice stem dipping method. After 41-generation selection with fipronil in 43 generations, the resistance level of the small brown planthopper (a population of L. striatellus from the wheat fields in Wuxi City, Jiangsu Province in 2005) to fipronil increased from 8. 4- to 230. 5-fold compared with the susceptible strain, and the realized heritability (h~2) of resistance at different selection stages according to the method described by Tabashnik was estimated as 0. 0388 (F_1 to F_(31)), 0.2636 (F_(32) to F_(43)) and 0. 1113(F_1 to F_(43)) , respectively. It is suggested that the L. striatellus had the definite risk of resistance to fipronil. The susceptibilities to 15 insecticides were evaluated in two field populations of L. striatellus, collected from Wuxi City, Jiangsu Province and Changxing County, Zhejiang Province in 2007 and 2008 by the rice stem dipping method. The results showed that the highest toxic insecticides to the insect was a phenyl-pyrazole class of insecticides including fipronil, butylene-fipronil and ethiprole ( LC_(50): 0. 2-1.7 mg/L), followed by thiamethoxam, nitenpyram, py-metrozine and chlorpyri/os (LC_(50):1. 7-9. 7 mg/L), and toxicity of the others was lower. According to a year-to-year variation in susceptibility, it was noted that susceptibilities to fipronil and ethiprole in L. striatellus population from Wuxi City in 2008 were reduced with a reducing rate of 1. 1-fold, compared with those to the two insecticides in 2007. Fipronil was used widely for controlling this insect in recent years, but ethiprole wasn't. Therefore, it seems that the L. striatellus population whose susceptibility to fipronil was reduced had cross-resistance to ethiprole. Inheritance characteristic of resistance to fipronil in L. slriatellus was studied through assaying dose-response data of the resistant (R) and susceptible(S) parents, reciprocal crosses (F_1 , F_1''), self-bred (F_2) and backcross (BC) progenies to fipronil by the rice stem dipping method, and the results showed that the resistance to fipronil in L. slriatellus was found to be polygenic, autosomal and inherited as a partial dominance trait [D(F_1) =0. 20,D(F_1') =0.38], Resistance management in L. striatellus was also discussed.