CAJ | 학술논문

应用结晶紫染色法细胞毒性试验和胞质分裂阻滞法微核试验(CBMNT)评价饮水消毒副产物(DBPs)的HepG2细胞毒性和遗传毒性,探讨该2种试验方法纳入饮水安全性评价体系的可行性.根据遗传毒性和致癌性选用了5种DBPs,分别对HepG2细胞进行染毒,结果表明,5种DBPs的细胞毒性大小排序为3-氯-4-二氯甲基-5-羟基-2(5氧)-呋喃酮(MX)>二氯乙腈>二溴己酸>二氯乙酸>三氯乙酸,其细胞毒性潜力(%C1/2值)分别为89.74,253.81,1217.02,5066.81,9335.38μmol/L,该5种DBPs在一定浓度下均可致HepG2细胞微核率显著增加,表现出明显的遗传毒性,其遗传损伤能力排序与细胞毒性大小相同.可考虑将这两种实验方法纳入到饮水安全性评价体系中.
응용결정자염색법세포독성시험화포질분렬조체법미핵시험(CBMNT)평개음수소독부산물(DBPs)적HepG2세포독성화유전독성,탐토해2충시험방법납입음수안전성평개체계적가행성.근거유전독성화치암성선용료5충DBPs,분별대HepG2세포진행염독,결과표명,5충DBPs적세포독성대소배서위3-록-4-이록갑기-5-간기-2(5양)-부남동(MX)>이록을정>이추기산>이록을산>삼록을산,기세포독성잠력(%C1/2치)분별위89.74,253.81,1217.02,5066.81,9335.38μmol/L,해5충DBPs재일정농도하균가치HepG2세포미핵솔현저증가,표현출명현적유전독성,기유전손상능력배서여세포독성대소상동.가고필장저량충실험방법납입도음수안전성평개체계중.
Cytotoxicity and genotoxicity of drinking water disinfection by-products (DBPs) in HepG2 cells was to evaluate using crystal violet-colorimetric assay and the cytokinesis-block micronucleus test, and the feasibility of introducing these assays into evaluation system for drinking water safty was explored. HepG2 cells were treated with five DBPs which were chosen based on their genotoxicity and carcinogenicity. The descending rank order in cytotoxicity was 3- chloro -4 - (dichloromethyl) -5 hydroxy -2 (5 H) - furanone (MX) > dichloroacetonitrile (DCAN) > dibromoacetic acid (DBA) > dichloroacetic acid ( DCA ) > trichloroacetic acid (TCA), with a value of cytotoxic potency (% C 1/2 values) ranged from 89.74 (MX) to 9335.38 (TCA) μmol/L. Micronuclei occurring frequency significantly increased in HepG2 cells treated with five DBPs. The rank order of their genotoxicity among the five DBPs was the same as that of their cytotoxicity. The results indicated that these two assays ate feasible for assessing the drinking water safety.