CAJ | 학술논문

目的在慢性胰腺炎动物模型,观察在体胰腺星状细胞的超微结构特点.方法 Sprague-Dawley大鼠随机分为实验组(n=12)和对照组(n=12).实验组给予16周高脂饮食诱导建立大鼠胰腺纤维化动物模型,对照组常规喂养.用HE和天狼猩红饱和苦味酸染色法观察胰腺组织病理学改变,免疫组化法识别胰腺组织中活化态及非活化态胰腺星状细胞,之后进一步通过透射电镜和免疫电镜方法对胰腺星状细胞的超微结构进行观察,最终对周细胞和泡心细胞进行形态学鉴别.结果病理形态学观察表明,长期高脂饮食可以诱导大鼠胰腺组织发生纤维化改变.实验组胰腺组织中活化态与非活化态胰腺星状细胞共存,细胞数量都较正常组明显增加.结论胰腺星状细胞在体超微结构特征的研究将为进一步研究其功能奠定形态学基础.
목적 재만성이선염동물모형,관찰재체이선성상세포적초미결구특점.방법 Sprague-Dawley대서수궤분위실험조(n=12)화대조조(n=12).실험조급여16주고지음식유도건립대서이선섬유화동물모형,대조조상규위양.용HE화천랑성홍포화고미산염색법관찰이선조직병이학개변,면역조화법식별이선조직중활화태급비활화태이선성상세포,지후진일보통과투사전경화면역전경방법대이선성상세포적초미결구진행관찰,최종대주세포화포심세포진행형태학감별.결과 병리형태학관찰표명,장기고지음식가이유도대서이선조직발생섬유화개변.실험조이선조직중활화태여비활화태이선성상세포공존,세포수량도교정상조명현증가.결론 이선성상세포재체초미결구특정적연구장위진일보연구기공능전정형태학기출.
Objective To investigate the morphological and ultrastructural characteristics of pancreatic stellate cells (PSCs) in a chronic pancreatitis murine model.Methods Sprague-Dawley rats were divided into experimental group (n=12) and control group (n=12).The experimental group was fed with a high-fat diet (HFD) for 16 weeks whereas the control group received regular rat feed.Pathologic changes in the pancreas were observed by several morphological studies including a Sirius Red staining method,as well as immunostaining for desmin and a smooth muscle actin (a-SMA).TEM and immunoelectron microscopy were performed to study the ultrastructure of the PSCs.Results The number of desmin and α-SMA positive cells significantly increased in the HFD groups compared to the control group.The quiescent and activated PSC cells both coexisted in the pancreas in the HFD group.Conclusions The morphology and ultrastructural study of pancreatic stellate cells may be helpful for further functional studies on chronic pancreatic fibrosis.