CAJ | 학술논문

目的检测趋化因子受体3(CXCR3)在类风湿关节炎(RA)患者外周血单一核细胞(PBNC)中的表达,并分析其与疾病活动性的相关性.方法研究建立实时荧光定量(RFQ)-聚合酶链反应(PCR)法测定外周血单个核细胞CXCR3 mRNA含量的方法;检测CXCR3在51例RA患者PBMC中的表达水平,并和32名健康人群进行对照.计量资料采用t检验、单因素方差分析,计数资料采用x2检验或Fisher's精确概率法.结果 RA患者在外周血的CXCR3 mRNA的表达水平明显高于健康对照组(8.8±1.5和7.3±1.9,P<0.05).在活动期与缓解期RA组的比较中发现活动期RA患者CXCR3 mRNA的表达水平也明显高于缓解期RA患者(9.4±1.0和8.0±1.6,P<0.05).而且活动期RA患者中外周血CXCR3 mRNA 表达水平与红细胞沉降率(ESR)、C反应蛋白(CRP)有密切的相关性,且存在正相关关系(r=0.824和0.765,P<0.05).RA患者中外周血CXCR3 mRNA表达水平与类风湿因子(RF)、抗角蛋白抗体(AKA)、抗核周因子(APF)抗体及抗环瓜氨酸多肽(CCP)抗体无显著相关性(P>0.05).结论 RFQ-PCR检测CXCR3 mRNA含量的方法具有较好的灵敏度和可重复性;RA患者CXCR3 mRNA表达含量升高,提示CXCR3可能参与了RA的发病过程.活动期RA患者CXCR3水平明显升高,并且和ESR、CRP呈正相关,提示CXCR3可作为观察病情活动性的指标和疗效、评价预后的指标.
목적 검측추화인자수체3(CXCR3)재류풍습관절염(RA)환자외주혈단일핵세포(PBNC)중적표체,병분석기여질병활동성적상관성.방법 연구건립실시형광정량(RFQ)-취합매련반응(PCR)법측정외주혈단개핵세포CXCR3 mRNA함량적방법;검측CXCR3재51례RA환자PBMC중적표체수평,병화32명건강인군진행대조.계량자료채용t검험、단인소방차분석,계수자료채용x2검험혹Fisher's정학개솔법.결과 RA환자재외주혈적CXCR3 mRNA적표체수평명현고우건강대조조(8.8±1.5화7.3±1.9,P<0.05).재활동기여완해기RA조적비교중발현활동기RA환자CXCR3 mRNA적표체수평야명현고우완해기RA환자(9.4±1.0화8.0±1.6,P<0.05).이차활동기RA환자중외주혈CXCR3 mRNA 표체수평여홍세포침강솔(ESR)、C반응단백(CRP)유밀절적상관성,차존재정상관관계(r=0.824화0.765,P<0.05).RA환자중외주혈CXCR3 mRNA표체수평여류풍습인자(RF)、항각단백항체(AKA)、항핵주인자(APF)항체급항배과안산다태(CCP)항체무현저상관성(P>0.05).결론 RFQ-PCR검측CXCR3 mRNA함량적방법구유교호적령민도화가중복성;RA환자CXCR3 mRNA표체함량승고,제시CXCR3가능삼여료RA적발병과정.활동기RA환자CXCR3수평명현승고,병차화ESR、CRP정정상관,제시CXCR3가작위관찰병정활동성적지표화료효、평개예후적지표.
Objective To detect the expression of chemokine receptors CXCR3 mRNA in the peripheral blood mononuclear cells (PBNCs) of patients with Rheumatoid Arthritis (RA) and to analyze the relationship between the expression and the disease activity. Methods mRNA was extracted from PBNCs and the expression of CXCR3 mRNA was detected by real-time fluorescence quantitative PCR (RFQ-PCR) in 51 RA patients and 32 controls. T-test, x2-test, ANOVA were used for statistial analysis. Results Comparison between the two groups had shown that the expression levels of CXCR3 mRNA in clinical active RA group were higher than those of the RA patients in remission and healthy controls (P<0.05). The expression levels of CXCR3 mRNA were positively correlated with serum levels of ESR and CRP in clinical active RA group (r=0.824, r=0.765, P<0.05). In addition, RF titer, APF, AKA, and anti-CCP had no significant correlation with the expression levels of CXCR3 mRNA in RA patients (P>0.05). Conclusion RFQ-PCR is a sensitive,reproducible and practical test. The mRNA expressions of CXCR3 are significantly elevated in RA patients,which suggest that CXCR3 may be involved in the pathogenesis of RA. The mRNA expressions of CXCR3 in active RA patients are higher than those of RA patients in remission. These results indicate that CXCR3 may play an important role in the pathogenesis and progression of RA, and CXCR3 may be considered as an indicator for disease activity, therapeutic efficacy and prognosis of RA.