中国医药
中國醫藥
중국의약
CHINA MEDICINE
2011年
4期
466-469
,共4页
朱王亮%郑玉云%陈强%柳景华
硃王亮%鄭玉雲%陳彊%柳景華
주왕량%정옥운%진강%류경화
代谢综合征%FOXC2-512C＞T基因%基因多态性
代謝綜閤徵%FOXC2-512C＞T基因%基因多態性
대사종합정%FOXC2-512C＞T기인%기인다태성
Metabolic syndrome%FOXC2-512C ＞T gene%Gene polymorphism
目的 探讨蒙古族人群代谢综合征(MS)发病的遗传机制和遗传易感性,为MS的早期预防及诊断治疗提供科学依据.方法 通过采用等位基因序列特异性聚合酶链反应法检测FOXC2-512C＞T基因多态性,比较MS组与正常对照组间基因型和等位基因频率分布,同时通过比较不同基因型间BMI、腰臀比、血压、TG、空腹血糖、高密度脂蛋白(HDL)水平差异,分析FOXC2-512C＞T基因多态性与MS的关系.结果 MS组腹围、臀围、BMI、空腹血糖、TG、TC、SBP、DBP均明显高于对照组,HDL值明显低于对照组,差异均有统计学意义(P＜0.05).MS组及对照组FOXC2-512C＞T基因分布均符合Hardy-Weinberg遗传平衡(x2=1.21,P=0.28;x2=1.51,P=0.20).MS组FOXC2-512C＞T基因C/C、T/C、T/T频率分别为26.7%、58.3%、15.0%,对照组为13.9%、39.3%、46.7%;MS组C、T等位基因频率为55.8%、44.2%;对照组为33.6%、66.4%.2组间基因型分布比较及等位基因分布比较,差异均具有统计学意义(均P＜0.05).多元逐步Logistic回归分析显示,在包头地区蒙古族人群中,FOXC2-512C＞T基因型、空腹血糖、TG、血压、BMI是MS发病的危险因素.结论 FOXC2-512C＞T基因型对MS的发生有较高的危险性,其很有可能成为MS治疗的新靶点.但FOXC2-512C＞T基因是否可作为中国内蒙古地区蒙古族人群MS发病的分子遗传学标志,还有待进一步大样本的研究予以证实.
目的 探討矇古族人群代謝綜閤徵(MS)髮病的遺傳機製和遺傳易感性,為MS的早期預防及診斷治療提供科學依據.方法 通過採用等位基因序列特異性聚閤酶鏈反應法檢測FOXC2-512C＞T基因多態性,比較MS組與正常對照組間基因型和等位基因頻率分佈,同時通過比較不同基因型間BMI、腰臀比、血壓、TG、空腹血糖、高密度脂蛋白(HDL)水平差異,分析FOXC2-512C＞T基因多態性與MS的關繫.結果 MS組腹圍、臀圍、BMI、空腹血糖、TG、TC、SBP、DBP均明顯高于對照組,HDL值明顯低于對照組,差異均有統計學意義(P＜0.05).MS組及對照組FOXC2-512C＞T基因分佈均符閤Hardy-Weinberg遺傳平衡(x2=1.21,P=0.28;x2=1.51,P=0.20).MS組FOXC2-512C＞T基因C/C、T/C、T/T頻率分彆為26.7%、58.3%、15.0%,對照組為13.9%、39.3%、46.7%;MS組C、T等位基因頻率為55.8%、44.2%;對照組為33.6%、66.4%.2組間基因型分佈比較及等位基因分佈比較,差異均具有統計學意義(均P＜0.05).多元逐步Logistic迴歸分析顯示,在包頭地區矇古族人群中,FOXC2-512C＞T基因型、空腹血糖、TG、血壓、BMI是MS髮病的危險因素.結論 FOXC2-512C＞T基因型對MS的髮生有較高的危險性,其很有可能成為MS治療的新靶點.但FOXC2-512C＞T基因是否可作為中國內矇古地區矇古族人群MS髮病的分子遺傳學標誌,還有待進一步大樣本的研究予以證實.
목적 탐토몽고족인군대사종합정(MS)발병적유전궤제화유전역감성,위MS적조기예방급진단치료제공과학의거.방법 통과채용등위기인서렬특이성취합매련반응법검측FOXC2-512C＞T기인다태성,비교MS조여정상대조조간기인형화등위기인빈솔분포,동시통과비교불동기인형간BMI、요둔비、혈압、TG、공복혈당、고밀도지단백(HDL)수평차이,분석FOXC2-512C＞T기인다태성여MS적관계.결과 MS조복위、둔위、BMI、공복혈당、TG、TC、SBP、DBP균명현고우대조조,HDL치명현저우대조조,차이균유통계학의의(P＜0.05).MS조급대조조FOXC2-512C＞T기인분포균부합Hardy-Weinberg유전평형(x2=1.21,P=0.28;x2=1.51,P=0.20).MS조FOXC2-512C＞T기인C/C、T/C、T/T빈솔분별위26.7%、58.3%、15.0%,대조조위13.9%、39.3%、46.7%;MS조C、T등위기인빈솔위55.8%、44.2%;대조조위33.6%、66.4%.2조간기인형분포비교급등위기인분포비교,차이균구유통계학의의(균P＜0.05).다원축보Logistic회귀분석현시,재포두지구몽고족인군중,FOXC2-512C＞T기인형、공복혈당、TG、혈압、BMI시MS발병적위험인소.결론 FOXC2-512C＞T기인형대MS적발생유교고적위험성,기흔유가능성위MS치료적신파점.단FOXC2-512C＞T기인시부가작위중국내몽고지구몽고족인군MS발병적분자유전학표지,환유대진일보대양본적연구여이증실.
Objective To analyze the genetic mechanism and susceptibility of metabolic syndrome (MS) in the Mongolian in order to provide evidence for the early prevention, diagnosis and treatment of MS. Methods We used PCR-SSP to detect FOXC2-512C ＞ T gene polymorphism to compare the difference of frequency of the genotype and allele between the MS group and the control group. The relationships between FOXC2-512C ＞ T gene polymorphism and MS were analyzed through comparison of BMI, WHR, BP, TG, glucose and HDL among different genotypes. Results Significant larger or higher abdominal circumference, hip, BMI, glucose, TG, TC, SBP and DBP but lower HDL were observed in the MS group compared to those in the control group(P ＜0.05). FOXC2-512C ＞T gene distribution in the MS and control group conformed to Hardy-Weinberg equilibrium (x2 = 1.21, P =0.28 ;x2= 1.51 ,P =0.20 respectively). In the MS group, the frequency of C/C, T/C and T/T in the FOXC2-512C ＞ T gene was 26.7%, 58.3% and 15.0% respectively. However, the frequency in the control group was 13.9%, 39.3% and 46.7% respectively( P ＜0.05). The frequency of C and T allele between the MS and control group were significantly different( P ＜0.05 ). Multivariable Logistic regression results showed that FOXC2-512C ＞ T genotype,FBG, TG, BP and BMI were risk factors of MS in the Mongolian. Conclusions The study shows that T allele might be the protective factor of MS while C allele might be the risk factor. FOXC2-512C ＞T genotype is a highly risk factor of MS, which might be the new target of the therapy for MS. However, further larger study is warranted to verify whether FOXC2-512C ＞ T gene can be molecular genetic marker of Inner Mongolia in China.
