CAJ | 학술논문

目的探讨Sema4D和人类表皮生长因子受体2(HER-2)在乳腺癌组织中的表达及临床意义.方法采用免疫组织化学SP法检测110例乳腺癌组织中Sema4D和HER-2的表达,并检测肿瘤微淋巴管密度(Microlymphtic vessel density,MLVD).结果乳腺癌组织中Sema4D和HER-2 蛋白的阳性表达率分别为71.82％ (79/110)和33.64％ (37/110),与对照组比较差异有统计学意义(P＜0.01)；淋巴结转移阳性组的Sema4D阳性表达率和MLVD值显著高于淋巴结转移阴性组(P＜0.01)；Sema4D和HER-2阳性表达与组织学分级、TNM分期、淋巴结转移及ER表达状态显著相关(P＜0.05),而与肿瘤大小及PR表达状态无关(P＞0.05).相关分析结果显示Sema4D与HER-2表达呈正相关(r=0.535,P＜0.01)；单因素生存分析显示,Sema4D高表达组的5年生存率(74.19％)高于低表达组(53.16％),其差异有统计学意义(P＜0.05).结论 Sema4D可能对乳腺癌的发生、发展起重要作用.
목적 탐토Sema4D화인류표피생장인자수체2(HER-2)재유선암조직중적표체급림상의의.방법 채용면역조직화학SP법검측110례유선암조직중Sema4D화HER-2적표체,병검측종류미림파관밀도(Microlymphtic vessel density,MLVD).결과 유선암조직중Sema4D화HER-2 단백적양성표체솔분별위71.82％ (79/110)화33.64％ (37/110),여대조조비교차이유통계학의의(P＜0.01)；림파결전이양성조적Sema4D양성표체솔화MLVD치현저고우림파결전이음성조(P＜0.01)；Sema4D화HER-2양성표체여조직학분급、TNM분기、림파결전이급ER표체상태현저상관(P＜0.05),이여종류대소급PR표체상태무관(P＞0.05).상관분석결과현시Sema4D여HER-2표체정정상관(r=0.535,P＜0.01)；단인소생존분석현시,Sema4D고표체조적5년생존솔(74.19％)고우저표체조(53.16％),기차이유통계학의의(P＜0.05).결론 Sema4D가능대유선암적발생、발전기중요작용.
Objective To investigate the expressions of Sema4D and HER-2 in breast cancers and their relationship with microlymphtic vessel density (MLVD).Methods MLVD and expressions of Sema4D and HER-2 were detected in 110 cases of breast cancer tissues by immunohistochemistry.The relationship of Sema4D and HER-2 expressions with clinicopathologic parameters was analyzed.Results The positive expression rate of Sema4D and HER-2 was 71.82％ (79/110) and 33.64％ (37/110),respectively,with a statistically significant differences compared to those in the control group ( P ＜0.01 ).The positive expression rates of Sema4D and MLVD in cases with lymphatic metastasis were higher than that without lymphatic metastasis ( P ＜ 0.01 ).The expressions of Sema4D and HER-2 were closely correlated with the histological grade,TNM stage,lymph node metastasis,and expression of ER of breast cancers ( P ＜0.05),but were not related to tumor size and expression of PR (P ＞ 0.05 ).On univariate analysis,the disease-free survival rate of patients with Sema4D positive expression was better than those with its negative expression ( P ＜ 0.05).Sema4D expression was positively correlated with the HER-2 expression ( r =0.535,P ＜ 0.01 ).Conclusions Sema4D may play important roles in the carcinogenesis and development of a breast cancer.