中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2012年
3期
254-257
,共4页
沈强%张学东%宗元娟%陈雪梅
瀋彊%張學東%宗元娟%陳雪梅
침강%장학동%종원연%진설매
糖尿病视网膜病变/预防和控制%血视网膜屏障/病理学%环孢菌素/治疗应用%疾病模型,动物
糖尿病視網膜病變/預防和控製%血視網膜屏障/病理學%環孢菌素/治療應用%疾病模型,動物
당뇨병시망막병변/예방화공제%혈시망막병장/병이학%배포균소/치료응용%질병모형,동물
Diabetic retinopathy/prevention & control%Blood-retinal barrier/pathology%Cyclosporine/therapeutic use%Disease models,animal
目的 观察和探讨玻璃体腔注射环孢菌素A(CsA)对糖尿病(DM)大鼠血视网膜屏障(BRB)的影响及其作用机制.方法 8～10周龄健康Sprague-Dawley雄性大鼠48只,随机分为正常对照组、DM组、CsA组及二甲基亚砜(DMSO)组,每组12只.对DM、CsA及DMSO组大鼠进行一次性腹腔注射链脲佐菌素(STZ)溶液建模,正常对照组大鼠腹腔注射等量的柠檬酸钠缓冲液.注射后72h,采用免疫组织化学染色法检测视网膜内外渗的内源性白蛋白评价BRB的渗透性；蛋白质免疫印迹法(Western blot)检测视网膜细胞间粘附分子(ICAM-1)的蛋白表达；酶联免疫吸附试验(ELISA)检测视网膜血管内皮生长因子(VEGF)的表达.结果 与正常对照组相比,DM组大鼠视网膜外渗的白蛋白增多,视网膜内ICAM-1、VEGF表达明显升高,差异均有统计学意义(F=29.350,29.240,9.658;P＜0.01).与DM组相比,CsA组大鼠视网膜外渗的白蛋白减少,视网膜内ICAM-1、VEGF表达明显下降,差异均有统计学意义(t=3.174,5.000,3.352；P＜0.05)；DMSO组大鼠视网膜外渗的白蛋白、视网膜内ICAM-1及VEGF表达均无明显变化,差异无统计学意义(t=0.420,0.561,0.312 ;P＞0.05).结论 玻璃体腔注射CsA对DM大鼠BRB具有保护作用.其机制可能与CsA降低ICAM-1及VEGF的表达有关.
目的 觀察和探討玻璃體腔註射環孢菌素A(CsA)對糖尿病(DM)大鼠血視網膜屏障(BRB)的影響及其作用機製.方法 8～10週齡健康Sprague-Dawley雄性大鼠48隻,隨機分為正常對照組、DM組、CsA組及二甲基亞砜(DMSO)組,每組12隻.對DM、CsA及DMSO組大鼠進行一次性腹腔註射鏈脲佐菌素(STZ)溶液建模,正常對照組大鼠腹腔註射等量的檸檬痠鈉緩遲液.註射後72h,採用免疫組織化學染色法檢測視網膜內外滲的內源性白蛋白評價BRB的滲透性；蛋白質免疫印跡法(Western blot)檢測視網膜細胞間粘附分子(ICAM-1)的蛋白錶達；酶聯免疫吸附試驗(ELISA)檢測視網膜血管內皮生長因子(VEGF)的錶達.結果 與正常對照組相比,DM組大鼠視網膜外滲的白蛋白增多,視網膜內ICAM-1、VEGF錶達明顯升高,差異均有統計學意義(F=29.350,29.240,9.658;P＜0.01).與DM組相比,CsA組大鼠視網膜外滲的白蛋白減少,視網膜內ICAM-1、VEGF錶達明顯下降,差異均有統計學意義(t=3.174,5.000,3.352；P＜0.05)；DMSO組大鼠視網膜外滲的白蛋白、視網膜內ICAM-1及VEGF錶達均無明顯變化,差異無統計學意義(t=0.420,0.561,0.312 ;P＞0.05).結論 玻璃體腔註射CsA對DM大鼠BRB具有保護作用.其機製可能與CsA降低ICAM-1及VEGF的錶達有關.
목적 관찰화탐토파리체강주사배포균소A(CsA)대당뇨병(DM)대서혈시망막병장(BRB)적영향급기작용궤제.방법 8～10주령건강Sprague-Dawley웅성대서48지,수궤분위정상대조조、DM조、CsA조급이갑기아풍(DMSO)조,매조12지.대DM、CsA급DMSO조대서진행일차성복강주사련뇨좌균소(STZ)용액건모,정상대조조대서복강주사등량적저몽산납완충액.주사후72h,채용면역조직화학염색법검측시망막내외삼적내원성백단백평개BRB적삼투성；단백질면역인적법(Western blot)검측시망막세포간점부분자(ICAM-1)적단백표체；매련면역흡부시험(ELISA)검측시망막혈관내피생장인자(VEGF)적표체.결과 여정상대조조상비,DM조대서시망막외삼적백단백증다,시망막내ICAM-1、VEGF표체명현승고,차이균유통계학의의(F=29.350,29.240,9.658;P＜0.01).여DM조상비,CsA조대서시망막외삼적백단백감소,시망막내ICAM-1、VEGF표체명현하강,차이균유통계학의의(t=3.174,5.000,3.352；P＜0.05)；DMSO조대서시망막외삼적백단백、시망막내ICAM-1급VEGF표체균무명현변화,차이무통계학의의(t=0.420,0.561,0.312 ;P＞0.05).결론 파리체강주사CsA대DM대서BRB구유보호작용.기궤제가능여CsA강저ICAM-1급VEGF적표체유관.
Objective To investigate the protective effect and mechanism of intravitreal injection with cyclosporin-A(CsA) on blood-retinal barrier (BRB) in diabetic rats.Methods A total of 48 SpragueDawley male mice at the age of 8-10 weeks were divided into normal group,diabetes mellitus (DM) group,CsA group and DMSO group,with 12 rats in each group.The rats in DM,CsA group and DMSO group were induced with streptozotocin (STZ) injection creating a diabetic retinopathy model.The same volume of citric sodium citrate buffer was injected into the rats in the normal group.Immunohistochemical staining was used to detect the BRB permeability,Western blot was used to measure the protein expression of intercellular adhesion molecule (ICAM-1).Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of vascular endothelial growth factor 72 hours after injection.Results Compared with the normal group,the BRB permeability,ICAM-1 and VEGF expression were significantly increased in DM group (F=29.350,29.240,9.658; P＜0.01).Compared with the DM group,the BRB permeability,ICAM-1 and VEGF expression were significantly decreased in CsA group (t=3.174,5.000,3.352; P＜0.05) ; but there was no obvious change of above indexes in DMSO group (t =0.420,0.561,0.312 ; P＞0.05).Conclusion Intravitreal injection of CsA has protective effects on BRB in diabetic rats.Downregulated expression of ICAM-1 and VEGF may be the mechanism.
