CAJ | 학술논문

目的：研究白桦三萜类物质(TBP)体内抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法：利用小鼠体内移植瘤模型，测定TBP对小鼠黑色素瘤B16、肉瘤S180、Lewis肺癌和艾氏腹水癌等肿瘤的抑瘤率。采用放射性掺入法检测ConA诱导的脾细胞增殖反应；放射性释放法检测巨噬细胞细胞毒活性；中性红释放法检测TNF和NK细胞活性。结果：TBP对各瘤株的抑瘤率均达到30％以上，对黑色素瘤B16的效果最好，抑瘤率为51．4％。TBP对ConA诱导的增殖反应和NK细胞活性无明显影响，但可促进巨噬细胞和脾细胞分泌TNF，增加巨噬细胞的细胞毒活性。结论：TBP具有良好的抗肿瘤作用，增强机体的非特异性免疫功能是其抗肿瘤机制之一。
목적：연구백화삼첩류물질(TBP)체내항종류작용급기대하류소서면역공능적영향。방법：이용소서체내이식류모형，측정TBP대소서흑색소류B16、육류S180、Lewis폐암화애씨복수암등종류적억류솔。채용방사성참입법검측ConA유도적비세포증식반응；방사성석방법검측거서세포세포독활성；중성홍석방법검측TNF화NK세포활성。결과：TBP대각류주적억류솔균체도30％이상，대흑색소류B16적효과최호，억류솔위51．4％。TBP대ConA유도적증식반응화NK세포활성무명현영향，단가촉진거서세포화비세포분비TNF，증가거서세포적세포독활성。결론：TBP구유량호적항종류작용，증강궤체적비특이성면역공능시기항종류궤제지일。
To study the antitumor activity and immunoregulatory effect of Triterpenes isolated from Betula Platyphylla (TBP)Methods: Inhihition rate (IR) on tumor growth was determined by the mice with transplantable tumors (melanoma B16, Sarcoma 180, Lewislung carcinoma and Ehrlich ascites cancer), splenocyte proliferation induced by ConA was measured by H-TdR incorporation; cytotoxicity ofmacrophage was observed by 3H-TdR release; activity of TNF and NK cell was determined by dye (natural red)release. Results: IR of 0.80 g/kg and 1.20 g/kg TBP on all of above tumor strains were greater than30％ in vivo. TBPdoes not show significant effect of splenocyte Prolifera-tion and NK cell activity, but significantly promoted the activity of TNF producted by macroghage and splenocyte. TBP also increase the cyto-toxicity of macrophage. Conclusion:TBP shows potent antitumor effect in vivo. Promoting nonspecific immunoactivity is one of the antitumormechanism of TBP.