动物营养学报
動物營養學報
동물영양학보
ACTA ZOONUTRIMENTA SINICA
2008年
4期
475-481
,共7页
章世元%俞路%王雅倩%孙怀昌%王志跃%王捍东%周联高%严桂芹%张莹%林显华%汪益峰%闫韩韩
章世元%俞路%王雅倩%孫懷昌%王誌躍%王捍東%週聯高%嚴桂芹%張瑩%林顯華%汪益峰%閆韓韓
장세원%유로%왕아천%손부창%왕지약%왕한동%주련고%엄계근%장형%림현화%왕익봉%염한한
pCEP4-PTH基因质粒%蛋鸡%生产性能%蛋壳质量%激素
pCEP4-PTH基因質粒%蛋鷄%生產性能%蛋殼質量%激素
pCEP4-PTH기인질립%단계%생산성능%단각질량%격소
pCEP4-PTH gene plasmid%Layers,Laying performance,Eggshell quality%Hormone
试验选用56周龄新扬州鸡产蛋鸡150只,随机分为3个处理,每个处理5个重复,各重复10只.研究经腿肌注射0、200和400μg鸡甲状旁腺素基因质粒(pCEP4-PTH)对蛋鸡生产性能、蛋壳质量和血液部分激素的影响.试验结果表明,pCEP4-PTH基因质粒可改善蛋鸡生产性能;200μg注射量可显著改善蛋壳强度(P<0.05),400μg注射量对蛋壳质量影响不显著;pCEP4-PTH基因质粒可在蛋鸡体内良好表达,甲状旁腺素(PTH)水平均显著升高(P<0.05),且注射400 μg时达到极显著水平(P<0.01),注射400 μg pCEP4-PTH基因质粒具有促进内源降钙素(CT)和雌激素(E2)分泌的作用(P<0.05),因而不利于其生物学效应的发挥,两试验组骨钙素(BGP)与对照组差异不显著,提示生产性能和蛋壳质量的改善未对骨骼造成不良影响.pCEP4-PTH基因质粒可改善蛋鸡生产性能和蛋壳质量,且不影响骨骼质量.
試驗選用56週齡新颺州鷄產蛋鷄150隻,隨機分為3箇處理,每箇處理5箇重複,各重複10隻.研究經腿肌註射0、200和400μg鷄甲狀徬腺素基因質粒(pCEP4-PTH)對蛋鷄生產性能、蛋殼質量和血液部分激素的影響.試驗結果錶明,pCEP4-PTH基因質粒可改善蛋鷄生產性能;200μg註射量可顯著改善蛋殼彊度(P<0.05),400μg註射量對蛋殼質量影響不顯著;pCEP4-PTH基因質粒可在蛋鷄體內良好錶達,甲狀徬腺素(PTH)水平均顯著升高(P<0.05),且註射400 μg時達到極顯著水平(P<0.01),註射400 μg pCEP4-PTH基因質粒具有促進內源降鈣素(CT)和雌激素(E2)分泌的作用(P<0.05),因而不利于其生物學效應的髮揮,兩試驗組骨鈣素(BGP)與對照組差異不顯著,提示生產性能和蛋殼質量的改善未對骨骼造成不良影響.pCEP4-PTH基因質粒可改善蛋鷄生產性能和蛋殼質量,且不影響骨骼質量.
시험선용56주령신양주계산단계150지,수궤분위3개처리,매개처리5개중복,각중복10지.연구경퇴기주사0、200화400μg계갑상방선소기인질립(pCEP4-PTH)대단계생산성능、단각질량화혈액부분격소적영향.시험결과표명,pCEP4-PTH기인질립가개선단계생산성능;200μg주사량가현저개선단각강도(P<0.05),400μg주사량대단각질량영향불현저;pCEP4-PTH기인질립가재단계체내량호표체,갑상방선소(PTH)수평균현저승고(P<0.05),차주사400 μg시체도겁현저수평(P<0.01),주사400 μg pCEP4-PTH기인질립구유촉진내원강개소(CT)화자격소(E2)분비적작용(P<0.05),인이불리우기생물학효응적발휘,량시험조골개소(BGP)여대조조차이불현저,제시생산성능화단각질량적개선미대골격조성불량영향.pCEP4-PTH기인질립가개선단계생산성능화단각질량,차불영향골격질량.
One hundred and fifty New Yangzhou chicken layers of 56-week-old were divided randomly into three treatment groups, with 5 duplicates of 10 birds in each group. The birds were treated with a dose of 0,200 and 400μg of pCEP4-PTH gene plasmid respectively by thigh muscle injection to investigate its effects on laying performance, eggshell quality and ser-um hormone levels. The results showed that the pCEP4-PTH gene plasmid was well expressed in layers, with the level of parathyrin elevated significantly in 200μg dose (P<0.05), and it elevated very significantly in 400μg dose (P<0.01), Th esecretion of endogenous calcitonin and estradiol could be promoted with dose of 400 μg (P＜0.05), which inhibited its bio-logical effects. The level of bone gla protein in the two treatment groups did not show any significant difference, indicating that the improvement of laying performance and eggshell quafity would not affect bone status, pCEP4-PTH gene plasmid im-proved the laying performance; the dose of 200 μg plasmid improved eggshell quality significantly (P<0.05), but no effect was observed in 400μg treatment (P>0.05). The experiment testified pCEP4-PTH gene plasmid improved the laying per-formance and eggshell quality, but did not affect bone status.
