国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
10期
952-954
,共3页
张红%施鑫鹤%杨海珍%尤崇革%李光迪
張紅%施鑫鶴%楊海珍%尤崇革%李光迪
장홍%시흠학%양해진%우숭혁%리광적
肝炎病毒%乙型%聚合酶链反应(PCR见)%肝功能试验
肝炎病毒%乙型%聚閤酶鏈反應(PCR見)%肝功能試驗
간염병독%을형%취합매련반응(PCR견)%간공능시험
Hepatitis B virus%Polymerase Chain Reaction%Liver Function Tests
目的 筛选乙肝抗病毒治疗监控新指标,为临床提供更多的信息,满足临床需要.方法 对临床确诊的87例慢性乙肝患者使用罗氏Lightcycler实时荧光定量PCR仪检测其血清病毒拷贝数,并以乙肝病毒载量为标准,分为高、中、低组进行研究;用速率法和免疫比浊法借助日立7600型全自动生化分析仪检测患者血清腺苷脱氨酶(adenosinedeaminase,ADA)、前清蛋白(prcalbumin,PA)、a-L-岩藻糖苷酶(alpha fucosidase,AFU)、总胆汁酸(total bile acid,TBA).结果 使用实时荧光定量PCR仪检测慢性乙肝患者血清巾的病毒拷贝数,据此可将患者血清HBV DNA检测结果分为A、B、C组,A组:103～104 copies/mL;B组:105～106 copies/mL;C组:＞107 copics/mL;分别检测ADA、PA、TBA、AFU指标,结果显示:每组ADA、PA、TBA、AFU指标与健康对照组间比较均有统计学意义(P＜0.01或P＜0.05),发现B组与A组、C组间比较PA水平显著下降(P＜0.01),TBA指标显著升高(P＜0.01),其余组间比较差异无统计学意义(P＞0.05).结论 ADA、PA、TBA、AFU指标检测可作为慢性乙肝患者抗病毒治疗的敏感指标,尤其B组患者通过ADA、PA、TBA、AFU联合检测,将有助于临床诊治.
目的 篩選乙肝抗病毒治療鑑控新指標,為臨床提供更多的信息,滿足臨床需要.方法 對臨床確診的87例慢性乙肝患者使用囉氏Lightcycler實時熒光定量PCR儀檢測其血清病毒拷貝數,併以乙肝病毒載量為標準,分為高、中、低組進行研究;用速率法和免疫比濁法藉助日立7600型全自動生化分析儀檢測患者血清腺苷脫氨酶(adenosinedeaminase,ADA)、前清蛋白(prcalbumin,PA)、a-L-巖藻糖苷酶(alpha fucosidase,AFU)、總膽汁痠(total bile acid,TBA).結果 使用實時熒光定量PCR儀檢測慢性乙肝患者血清巾的病毒拷貝數,據此可將患者血清HBV DNA檢測結果分為A、B、C組,A組:103～104 copies/mL;B組:105～106 copies/mL;C組:＞107 copics/mL;分彆檢測ADA、PA、TBA、AFU指標,結果顯示:每組ADA、PA、TBA、AFU指標與健康對照組間比較均有統計學意義(P＜0.01或P＜0.05),髮現B組與A組、C組間比較PA水平顯著下降(P＜0.01),TBA指標顯著升高(P＜0.01),其餘組間比較差異無統計學意義(P＞0.05).結論 ADA、PA、TBA、AFU指標檢測可作為慢性乙肝患者抗病毒治療的敏感指標,尤其B組患者通過ADA、PA、TBA、AFU聯閤檢測,將有助于臨床診治.
목적 사선을간항병독치료감공신지표,위림상제공경다적신식,만족림상수요.방법 대림상학진적87례만성을간환자사용라씨Lightcycler실시형광정량PCR의검측기혈청병독고패수,병이을간병독재량위표준,분위고、중、저조진행연구;용속솔법화면역비탁법차조일립7600형전자동생화분석의검측환자혈청선감탈안매(adenosinedeaminase,ADA)、전청단백(prcalbumin,PA)、a-L-암조당감매(alpha fucosidase,AFU)、총담즙산(total bile acid,TBA).결과 사용실시형광정량PCR의검측만성을간환자혈청건적병독고패수,거차가장환자혈청HBV DNA검측결과분위A、B、C조,A조:103～104 copies/mL;B조:105～106 copies/mL;C조:＞107 copics/mL;분별검측ADA、PA、TBA、AFU지표,결과현시:매조ADA、PA、TBA、AFU지표여건강대조조간비교균유통계학의의(P＜0.01혹P＜0.05),발현B조여A조、C조간비교PA수평현저하강(P＜0.01),TBA지표현저승고(P＜0.01),기여조간비교차이무통계학의의(P＞0.05).결론 ADA、PA、TBA、AFU지표검측가작위만성을간환자항병독치료적민감지표,우기B조환자통과ADA、PA、TBA、AFU연합검측,장유조우림상진치.
Objective To sereen new monitoring markers for antiviral treatment of hepatitis B,provide more information for the clinic.Methods HBV DNA copies in serum of 87 patients with chronic hepatitis B were detected by applying Roche LightCyeler RT-PCR system,while serum adenosine deaminase(ADA),prealbumin(PA),alpha fucosidase(AFU)and total bile acid(TBA)were measured with Hitachi 7600 automatic biochemical analyzer(velocity assay and immunoturbidimetry).Results Based on the viral load detected,all patients were divided into three groups;group A(103～104 copies/mL);group B(105～106 copies/mL)and group C(＞107 copies/mL).The serum levels of ADA,PA,AFU and TBA were all significantly higher in the three groups than those in healthy control group(P＜0.01 or P＜0.05).Compared with that in group A and gropu C,PA level was cvidently lower and TBA was markedly higher in group B(both P＜0.01).No statistical differences in other indices were found among the three groups(P＞0.05).Conclusion ADA,PA,AFU and TBA may be used as sensitive markers for antiviral treatment of hepatitis B,especially for patients of group B,combined detection of these markers contributes to clinical diagnosis and treatment of hepatitis B.
