CAJ | 학술논문

利用高效液相色谱仪及田间试验方法,建立了乙嘧酚在黄瓜和土壤中的残留分析方法,研究了乙嘧酚在黄瓜和土壤中的残留消解动态,对影响残留分析方法的主要参数进行了优化.黄瓜和土壤样品分别用乙腈和丙酮提取,硅胶柱净化,高效液相色谱仪二极管阵列检测器检测,外标法定量.结果表明,该方法的最小检出量为3.5×10-10g,在黄瓜和土壤中的最低检测浓度分别为0.010和0.005 mg·kg~(-1).乙嘧酚的平均添加回收率为80.5%～103.1%,变异系数为2.10%～3.74%.消解动态试验表明,乙嘧酚的残留量随时间延长而降低,消解动态曲线符合一级动力学方程,在黄瓜和土壤中的半衰期分别为3.5和9.9 d,属于易降解性农药化合物.乙嘧酚在黄瓜中消解速率高于其在土壤中的消解速率,这可能是由于黄瓜生长稀释作用导致的.
이용고효액상색보의급전간시험방법,건립료을밀분재황과화토양중적잔류분석방법,연구료을밀분재황과화토양중적잔류소해동태,대영향잔류분석방법적주요삼수진행료우화.황과화토양양품분별용을정화병동제취,규효주정화,고효액상색보의이겁관진렬검측기검측,외표법정량.결과표명,해방법적최소검출량위3.5×10-10g,재황과화토양중적최저검측농도분별위0.010화0.005 mg·kg~(-1).을밀분적평균첨가회수솔위80.5%～103.1%,변이계수위2.10%～3.74%.소해동태시험표명,을밀분적잔류량수시간연장이강저,소해동태곡선부합일급동역학방정,재황과화토양중적반쇠기분별위3.5화9.9 d,속우역강해성농약화합물.을밀분재황과중소해속솔고우기재토양중적소해속솔,저가능시유우황과생장희석작용도치적.
A simple, reliable and accurate method has been developed to determine ethirimol residue in cucumber and soil, and the degradation dynamics of ethirimol in cucumber and soil were studied. Parameters, affecting the performance of analysis such as absorption wavelength, mobile phase, amount of water added into soil and clean-up procedure, were studied and optimized. Under the optimum conditions of analysis (Cucumber and soil samples were extracted by acetonitrile and acetone, respectively; then, the extracted solution was cleaned up by silica gel cartridge and analyzed by HPLC equipped with DAD; finally, the residual content was quantified by ESTD), the performance of the method was evaluated. The results showed the limit of detection of the method was 3.5×10~(-10)g, and the limit of quantificaiton in cucumber and soil were 0.010 mg·L~(-1) and 0.005 mg·kg~(-1), respectively. Average fortified recovery of the method varied from 80.5% to 103.1% with the relative standard deviation varying from 2.10% to 3.74%. Furthermore, the degradation dynamics indicated ethirimol degraded along prolongation of time, the curve of degradation dynamics accorded with the first-order kinetics equation, and the half-lives of ethirimol in cucumber and soil were 3.5 day and 9.9 day, respectively. The degradation rate of ethirimol in cucumber was faster than that in soil, which probably resulted from the dilution effect of cucumber's growth. To sum up, ethirimol should be classified as easily degradable agrochemical and the gained parameters of degradation dynamics provided scientific basis for the safety evaluation on ethirimol.