CAJ | 학술논문

2008年秋,在上海郊区大棚栽培的豇豆上发现一种叶片褪绿和黄化病害症状.通过PCR从感病的植株叶片中扩增出番茄黄化曲叶病毒的全序列.序列比较及系统进化分析表明,这种病毒是菜豆金色黄花叶病毒属成员之一的Tomato Yellow Leaf Curl Virus-Israel(TYLCV-IL).基因组是由2781个核苷酸组成的环状单链DNA,具有典型的旧世界单组分菜豆金色黄花叶病毒属病毒的特征,有6个开放阅读框,V2和V1(外壳蛋白基因)在病毒链上,而C3、C2、C1和C4在互补链上.这是国内番茄黄化曲叶病毒侵染豇豆的首次报道.
2008년추,재상해교구대붕재배적강두상발현일충협편퇴록화황화병해증상.통과PCR종감병적식주협편중확증출번가황화곡협병독적전서렬.서렬비교급계통진화분석표명,저충병독시채두금색황화협병독속성원지일적Tomato Yellow Leaf Curl Virus-Israel(TYLCV-IL).기인조시유2781개핵감산조성적배상단련DNA,구유전형적구세계단조분채두금색황화협병독속병독적특정,유6개개방열독광,V2화V1(외각단백기인)재병독련상,이C3、C2、C1화C4재호보련상.저시국내번가황화곡협병독침염강두적수차보도.
In the fall of 2008,the cowpea growing in a greenhouse in Shanghai suburban suffered a disease characterized by chlorotic and yellowing leaves.The complete nucleotide sequence of a tomato yellow leaf curl virus was amplified from DNA extracted from diseased plants.The sequence comparisons and phylogenetic analysis revealed that the pathogen was Tomato yellow leaf curl virus-Israel(TYLCV-IL),a member of the genus Begomovirus.The genome consisted of circular ssDNA with 2781 nucleotides,which has the typical characteristic of Old World monopartite begomoviruses with six ORFs corresponding to V2 and V1 (Capsid Protein gene)on the viral strand,and C3,C2,C1 and C4 on the complementary strand.This is the first report that a natural Tomato yellow leaf curl virus infected V.sinensis.