中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2011年
3期
173-175
,共3页
江静娴%张敏敏%杨骅%吴洪玉%邹多武%李兆申
江靜嫻%張敏敏%楊驊%吳洪玉%鄒多武%李兆申
강정한%장민민%양화%오홍옥%추다무%리조신
胰腺肿瘤%主要组织相容性复合物%腺病毒%感染
胰腺腫瘤%主要組織相容性複閤物%腺病毒%感染
이선종류%주요조직상용성복합물%선병독%감염
Pancreatic neoplasms%Major histocompatibility complex%Adenoviruses%Infection
目的 研究CⅡTA基因对人胰腺癌CaPanC-2细胞和小鼠胰腺癌PanC-02细胞主要组织相容性复合物(MHC)Ⅱ类分子表达的影响.方法 将携带CⅢA基因的腺病毒(Ad-CⅡTA)分别感染人胰腺癌CaPanC-2细胞和小鼠胰腺癌PanC-02细胞,培养24、48、72 h.实时PCR法检测感染细胞CⅡTA mRNA的表达,蛋白质印迹法检测CⅡTA蛋白表达,流式细胞仪检测 MHCⅡ类分子阳性表达细胞百分比.结果 CaPanC-2细胞感染后24、48、72 h的CⅡTA mRNA表达量分别为对照组的(16769±6455)、(261568±348850)和(834816 ±97783)倍(P<0.05);PanC-02细胞为对照组的(548546±87755)、(1242684±624888)和(1401647±726145)倍(P<0.05).CaPanC-2和PanC-02细胞均不表达CⅡTA蛋白,感染后48 h,CaPanC-2、PanC-02细胞CⅢTA蛋白表达量为0.746±0.499和0.631±0.244.感染24、48、72 h组CaPanC-2细胞表达MHCⅡ类分子的细胞百分比分别为(8.1±0.3)%、(18.9±0.3)%、(78.5±0.9)%,显著高于对照组的(7.0±0.1)%(P<0.01);PanC-02细胞表达MHCⅡ类分子的细胞百分比分别为(5.1±0.2)%、(37.3 ±2.0)%、(68.8±2.2)%,显著高于对照组的(2.2±0.2)%(P<0.01).结论 Ad-CⅡTA体外感染胰腺肿瘤细胞可促进CⅡTA基因的表达,从而促进细胞表面MHC Ⅱ类分子的表达.
目的 研究CⅡTA基因對人胰腺癌CaPanC-2細胞和小鼠胰腺癌PanC-02細胞主要組織相容性複閤物(MHC)Ⅱ類分子錶達的影響.方法 將攜帶CⅢA基因的腺病毒(Ad-CⅡTA)分彆感染人胰腺癌CaPanC-2細胞和小鼠胰腺癌PanC-02細胞,培養24、48、72 h.實時PCR法檢測感染細胞CⅡTA mRNA的錶達,蛋白質印跡法檢測CⅡTA蛋白錶達,流式細胞儀檢測 MHCⅡ類分子暘性錶達細胞百分比.結果 CaPanC-2細胞感染後24、48、72 h的CⅡTA mRNA錶達量分彆為對照組的(16769±6455)、(261568±348850)和(834816 ±97783)倍(P<0.05);PanC-02細胞為對照組的(548546±87755)、(1242684±624888)和(1401647±726145)倍(P<0.05).CaPanC-2和PanC-02細胞均不錶達CⅡTA蛋白,感染後48 h,CaPanC-2、PanC-02細胞CⅢTA蛋白錶達量為0.746±0.499和0.631±0.244.感染24、48、72 h組CaPanC-2細胞錶達MHCⅡ類分子的細胞百分比分彆為(8.1±0.3)%、(18.9±0.3)%、(78.5±0.9)%,顯著高于對照組的(7.0±0.1)%(P<0.01);PanC-02細胞錶達MHCⅡ類分子的細胞百分比分彆為(5.1±0.2)%、(37.3 ±2.0)%、(68.8±2.2)%,顯著高于對照組的(2.2±0.2)%(P<0.01).結論 Ad-CⅡTA體外感染胰腺腫瘤細胞可促進CⅡTA基因的錶達,從而促進細胞錶麵MHC Ⅱ類分子的錶達.
목적 연구CⅡTA기인대인이선암CaPanC-2세포화소서이선암PanC-02세포주요조직상용성복합물(MHC)Ⅱ류분자표체적영향.방법 장휴대CⅢA기인적선병독(Ad-CⅡTA)분별감염인이선암CaPanC-2세포화소서이선암PanC-02세포,배양24、48、72 h.실시PCR법검측감염세포CⅡTA mRNA적표체,단백질인적법검측CⅡTA단백표체,류식세포의검측 MHCⅡ류분자양성표체세포백분비.결과 CaPanC-2세포감염후24、48、72 h적CⅡTA mRNA표체량분별위대조조적(16769±6455)、(261568±348850)화(834816 ±97783)배(P<0.05);PanC-02세포위대조조적(548546±87755)、(1242684±624888)화(1401647±726145)배(P<0.05).CaPanC-2화PanC-02세포균불표체CⅡTA단백,감염후48 h,CaPanC-2、PanC-02세포CⅢTA단백표체량위0.746±0.499화0.631±0.244.감염24、48、72 h조CaPanC-2세포표체MHCⅡ류분자적세포백분비분별위(8.1±0.3)%、(18.9±0.3)%、(78.5±0.9)%,현저고우대조조적(7.0±0.1)%(P<0.01);PanC-02세포표체MHCⅡ류분자적세포백분비분별위(5.1±0.2)%、(37.3 ±2.0)%、(68.8±2.2)%,현저고우대조조적(2.2±0.2)%(P<0.01).결론 Ad-CⅡTA체외감염이선종류세포가촉진CⅡTA기인적표체,종이촉진세포표면MHC Ⅱ류분자적표체.
Objective To investigate the effect of MHC class Ⅱ transactivator( MHC Ⅱ TA ) on MHC Ⅱ expression of human and rat pancreatic cancer cell line CaPanC-2 and PanC-02. Methods The recombinant adenovirus (Ad-C Ⅱ TA) was transfected into the CaPanC-2 cell and the PanC-02 cell, then it was cultured for 24, 48, 72 h. The C Ⅱ TA mRNA expressions were assayed by Real Time PCR and CⅡ TA protein expressions were determined by Western blotting. The percentage of positive expression cells of MHC Ⅱ were measured by flow cytometry. Results The C Ⅱ TA mRNA expressions of CaPanC-2 cell at 24, 48, 72 h were 16769 ± 6455, 261568 ±348850 and 834816 ±97783 folds higher than that of control group (P <0.05). The CⅡTA mRNA expressions of PanC-02 was 548546 ± 87755, 1242684 ± 624888 and 1401647 ± 726145 folds higher than that of control group ( P <0.05). CⅡ TA protein was not expressed in CaPanC-2 cell and PanC-02 cell. After transfection for 48 h, the CⅡ TA protein expressions of CaPanC-2 and PanC-02 were 0.746 ± 0.499 and 0.631 ±0.244. The percentage of positive expression cells of MHC Ⅱ in CaPanC-2 cells after 24, 48, 72 h were (8.1±0.3)%, (18.9±0.3)%, (78.5±0.90%, which were significantly higher than that in control group [ ( 7.0 ± 0.1)% , P < 0.01 . The percentage of positive expression cells of MHC Ⅱ in PanC - 0 2 were (5.1 ±0.2)% , (37.3 ±2.0)% , (68.8 ±2.2)%, which were significantly higher than that in control group [ (2.2 ±0.2)% , P <0.01). Conclusions Transfection of Ad-C Ⅱ TA could increase the expression of CⅡTA and MHC Ⅱ molecules of pancreatic tumor cells in vitro.
