CAJ | 학술논문

目的观察庆大霉素-德州红耦联物在体外培养小鼠耳蜗Corti器的分布情况,比较不同时间庆大霉素吸收的差异.方法分离小鼠耳蜗Corti器,体外培养,培养液中加有庆大霉素-德州红耦联物,分别于培养后3 h、6 h、12 h、24 h、48 h、3 d.4 d和7 d收集标本,荧光染色后行激光扫描共聚焦显微镜观察基底膜庆大霉素的分布情况.结果小鼠耳蜗Corti器在培养3 h后即可见外毛细胞的纤毛和胞体两侧的胞膜有庆大霉素分布;随着培养时间的延长,庆大霉素在Corti器中的所有细胞均见分布,尤以外毛细胞明显,主要聚集在毛细胞顶端纤毛的下方;培养24 h后庆大霉素在外毛细胞的聚集达到最高峰,而在支持细胞未见明显的聚集;体外培养7 d后在毛细胞胞质中仍可见庆大霉素分布.结论小鼠Corti器体外培养可用来动态检测庆大霉素在耳蜗细胞的吸收和聚集情况.
목적 관찰경대매소-덕주홍우련물재체외배양소서이와Corti기적분포정황,비교불동시간경대매소흡수적차이.방법 분리소서이와Corti기,체외배양,배양액중가유경대매소-덕주홍우련물,분별우배양후3 h、6 h、12 h、24 h、48 h、3 d.4 d화7 d수집표본,형광염색후행격광소묘공취초현미경관찰기저막경대매소적분포정황.결과 소서이와Corti기재배양3 h후즉가견외모세포적섬모화포체량측적포막유경대매소분포;수착배양시간적연장,경대매소재Corti기중적소유세포균견분포,우이외모세포명현,주요취집재모세포정단섬모적하방;배양24 h후경대매소재외모세포적취집체도최고봉,이재지지세포미견명현적취집;체외배양7 d후재모세포포질중잉가견경대매소분포.결론 소서Corti기체외배양가용래동태검측경대매소재이와세포적흡수화취집정황.
Objective To investigate the uptaking and accumulation of gentamicin by mouse hair cells in vitro.Methods Cochlear explants were prepared from the microdissected neonatM mouse cochlea. Cochlear explants were cultured with gentamicin-Texas-red conjunction (G1TrR) for difierent time.Laser confocal microscopy was used to observe the distribution of GTYR in the cochlear sensoty ceUs after labeling with phalloidin-alexa-488.ResulIs Soon after culture,there was diffuse red staining all tissue cells in the explants.At later time the hair cells were more staining tllan other cells in the explants.There was no obviously accumulation of GTTR in the supporting cells.The peak level of fluorescent density was reached at 24 hours culture.The GTTR was seen in the infracuticular zone of the hair cells.There was still accumulation of GTTR in the hair cells of the explants after 7 days culturing.Conclusions GTTR and Cochlea explants were useful methods to investigate the pharmacokinetics and mechanisms of gentamicin accumulation over time.