CAJ | 학술논문

目的探讨乳铁蛋白对神经病理性痛大鼠脊髓背角cGMP依赖性蛋白激酶(PKG)活性的影响.方法雄性SD大鼠32只,体重200～250 g,随机分为4组(n=8):假手术组仅分离坐骨神经,不结扎,鞘内注射生理盐水10μl+50%二甲基亚砜(DMSO)10μl;余3组采用结扎坐骨神经的方法制备大鼠神经病理性痛模型,神经病理性痛组鞘内注射生理盐水10μl+50%DMSO10μl;乳铁蛋白组鞘内注射乳铁蛋白100μg+50%DMS010μl;PKG抑制剂KT5823组鞘内注射乳铁蛋白100μg+KT582310μl.给药后180 min内每隔30 min以热刺激法测定大鼠缩爪潜伏期,随后处死大鼠取脊髓背角,采用免疫荧光法检测PKG活性,并行定量分析.结果与神经病理性痛组和KT5823组相比,乳铁蛋白组缩爪潜伏期延长,乳铁蛋白组脊髓背角PKG活性升高(P＜0.05);神经病理性痛组与KT5823组上述指标比较差异无统计学意义(P＞0.05).结论乳铁蛋白可通过抑制脊髓背角PKG活性减轻大鼠神经病理性痛.
목적 탐토유철단백대신경병이성통대서척수배각cGMP의뢰성단백격매(PKG)활성적영향.방법 웅성SD대서32지,체중200～250 g,수궤분위4조(n=8):가수술조부분리좌골신경,불결찰,초내주사생리염수10μl+50%이갑기아풍(DMSO)10μl;여3조채용결찰좌골신경적방법제비대서신경병이성통모형,신경병이성통조초내주사생리염수10μl+50%DMSO10μl;유철단백조초내주사유철단백100μg+50%DMS010μl;PKG억제제KT5823조초내주사유철단백100μg+KT582310μl.급약후180 min내매격30 min이열자격법측정대서축조잠복기,수후처사대서취척수배각,채용면역형광법검측PKG활성,병행정량분석.결과 여신경병이성통조화KT5823조상비,유철단백조축조잠복기연장,유철단백조척수배각PKG활성승고(P＜0.05);신경병이성통조여KT5823조상술지표비교차이무통계학의의(P＞0.05).결론 유철단백가통과억제척수배각PKG활성감경대서신경병이성통.
Objective To investigate the effects of lactoferrin on activity of PKG in spinal dorsal horn in a rat model of neuropathic pain(NP).Methods Thirty-two male SD rats weighing 200-250 g were randomly divided into4 groups(n = 8 each): sham operation group(group S),NP group,lactoferrin group and KT5823(an inhibitor of PKG)group.Neuropathic pain was produced by placing loosely constrictive ligatures around the common sciatic nerve in group NP,lactoferrin and KT5823,while the sciatic nerve was only exposed but not ligated in groupS.In group S and NP,normal saline 10 μl + 50% dimethyl sulfoxide(DMSO)10 μl were injected intrathecally.Lactoferrin 100 μg + 50% DMSO 10 μl were given intrathecally in group lactoferrin.Lactoferrin 100 μg + KT5823 10 μl were given intrathecally in group KT5823.The paw withdrawal latency(PWL)to a thermal nociceptive stimulus was measured every 30 min within 180 min after administration.The rats were then sacrificed and the spinal cord was removed.The activity of PKG in the spinal dorsal horn was determined by immunofluorescence.Results Compared with group NP and KT5823,the PWL was significantly prolonged after administration in group lactoferrin and the PKG activity was significantly increased in group lactoferrin(P ＜ 0.05).There was no significant difference in the parameters mentioned above between group NP and group KT5823(P ＞ 0.05).Conclusion Lactoferrin reduces NP by inhibiting the activity of PKG in spinal dorsal horn in rats.